本文選題：丹皮　切入點(diǎn)：赤芍　出處：《中草藥》2016年15期 　論文類型：期刊論文

【摘要】：目的觀察牡丹皮、赤芍與白芍對(duì)急性血瘀模型大鼠的影響,揭示三者在功效方面的異同,并探討牡丹皮、赤芍與白芍活血功效的機(jī)制。方法大鼠sc鹽酸腎上腺素結(jié)合冰水浸泡制作急性血瘀模型,以丹參注射液為陽(yáng)性對(duì)照,觀察牡丹皮、赤芍與白芍對(duì)血液流變學(xué)、凝血功能指標(biāo)、血小板系統(tǒng)、血清中血栓素B2(TXB2)、6-酮-前列腺素(6-keto-PGF1α)的影響。結(jié)果與模型組相比,牡丹皮能夠延長(zhǎng)急性血瘀模型大鼠的凝血酶原時(shí)間(PT)、凝血酶時(shí)間(TT)(P0.05),顯著抑制血小板黏附(P0.01),且上調(diào)血小板內(nèi)皮型一氧化氮合酶(e NOS)與Ser1177位磷酸化的e NOS(p-e NOS)蛋白表達(dá)水平(P0.01、0.001),減少血清中TXB2(P0.05)及增加6-keto-PGF1α的量(P0.01);赤芍能夠顯著改善不同切變率下血瘀模型大鼠的全血黏度,延長(zhǎng)部分活化凝血活酶時(shí)間(APTT)、PT、TT(P0.05、0.01),降低血小板黏附率(P0.05),減少血清中TXB2的量(P0.01)、增加6-keto-PGF1α的量(P0.01),上調(diào)血小板e(cuò) NOS、p-e NOS蛋白表達(dá)水平(P0.05);白芍則對(duì)血瘀模型大鼠的全血黏度的影響并不顯著,但能夠顯著延長(zhǎng)APTT、TT(P0.05),降低血小板黏附率(P0.05),增加血清中6-keto-PGF1α的量(P0.05),同時(shí)顯著增加血小板e(cuò) NOS、p-e NOS蛋白表達(dá)水平(P0.05)。結(jié)論牡丹皮、赤芍和白芍均具有不同程度改善血液流變性、凝血系統(tǒng)、抑制血小板黏附率、增加血小板e(cuò) NOS、p-e NOS蛋白表達(dá)水平,但牡丹皮、赤芍較白芍活血化瘀作用強(qiáng)。
[Abstract]:Objective to observe the effects of peony peel, red peony root and white peony root on acute blood stasis model rats, to reveal the similarities and differences among them, and to explore the effect of peony peel. Methods the acute blood stasis model was made in rats with Sc hydrochloric acid and ice water immersion. The peony skin, paeoniae rubra and Paeoniae Alba were used as positive control to observe the indexes of hemorheology and coagulation function of Paeonia rubra and Radix Paeoniae Alba and Radix Paeoniae Alba and Radix Paeoniae Alba. The effects of thromboxane B _ 2H _ 2T _ XB _ (2) -6-keto-PGF _ (1 偽) on the platelet system were compared with those in the model group. Peony skin can prolong the prothrombin time and thrombin time of acute blood stasis model rats. It can significantly inhibit the platelet adhesion P0.01C, and up-regulate the expression of platelet endothelial nitric oxide synthase (Enos) and Ser1177 phosphorylated e NOS(p-e NOSprotein. The whole blood viscosity of rats with blood stasis model was significantly improved by P0.01P _ (0.01) and the increase of 6-keto-PGF _ (1 偽) in serum by reducing serum TXB _ (2) P _ (0.05) and increasing 6-keto-PGF _ (1 偽), and the whole blood viscosity of rats with blood stasis model was significantly improved by Paeonia lanceolata at different shear rates. Prolonging partial activated thromboplastin time (P0.05), decreasing platelet adhesion rate (P0.05N), reducing the amount of TXB2 in serum (P0.01A), increasing the amount of 6-keto-PGF1 偽 (P0.01A), upregulating the expression level of platelet E-NOSS-p-e NOS protein (P0.05), while Paeonia lanceolata had no significant effect on the whole blood viscosity of rats with blood stasis model. But it can significantly prolong the level of P0.05, decrease the adhesion rate of P0.05, increase the amount of 6-keto-PGF _ 1 偽 in serum and increase the expression level of E-NOSP-e NOS protein. Conclusion Peony peony, Radix Paeoniae rubra and Radix Paeoniae Alba can improve the hemorheology and coagulation system in different degrees. The platelet adhesion rate was inhibited and the expression level of eNOSP-e NOS was increased, but peony peel and red peony had stronger effect on promoting blood circulation and removing blood stasis than Paeonia lanceolata.
【作者單位】： 江蘇省中醫(yī)藥研究院國(guó)家中醫(yī)藥管理局中藥釋藥系統(tǒng)重點(diǎn)研究室;江蘇大學(xué)藥學(xué)院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81202906) 江蘇省自然科學(xué)基金(BK2012491) 中央本級(jí)重大增減支項(xiàng)目(2060302) 國(guó)家自然基金重點(diǎn)項(xiàng)目(81130070)
【分類號(hào)】：R285.5;R-332

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