本文選題：髓間充質(zhì)干細(xì)胞　切入點(diǎn)：肝硬化　出處：《免疫學(xué)雜志》2017年07期 　論文類型：期刊論文

【摘要】：目的 探討骨髓間充質(zhì)干細(xì)胞移植治療對(duì)肝硬化大鼠自身免疫功能的影響,為臨床治療方法提供理論依據(jù)。方法選取150只健康雄性Wistar大鼠,隨機(jī)分為3組,即對(duì)照組(不采取任何干預(yù)措施)、肝硬化組(行大鼠肝硬化模型制備)、治療組(行大鼠肝硬化模型制備后給予骨髓間充質(zhì)干細(xì)胞移植治療)。檢測(cè)各組大鼠脾臟T、B淋巴細(xì)胞增殖情況,CD4~+CD25~+Foxp3~+調(diào)節(jié)性T細(xì)胞的水平,Foxp3 mRNA表達(dá)水平。結(jié)果 對(duì)照組大鼠肝細(xì)胞結(jié)構(gòu)完整,沒(méi)有纖維增生及假小葉存在;肝硬化組大鼠肝小葉結(jié)構(gòu)破壞,假小葉出現(xiàn),肝細(xì)胞水腫變性壞死,可見(jiàn)炎性細(xì)胞浸潤(rùn);實(shí)驗(yàn)組大鼠肝細(xì)胞水腫變性壞死程度較肝硬化組顯著降低,但仍可見(jiàn)增生的纖維組織。肝硬化組大鼠脾臟T、B淋巴細(xì)胞體外增殖情況明顯高于對(duì)照組(P0.05),經(jīng)BMSCs治療后,治療組大鼠脾臟T、B淋巴細(xì)胞體外增殖情況顯著低于肝硬化組(P0.05);肝硬化組大鼠脾臟Foxp3 mRNA的表達(dá)水平明顯低于對(duì)照組(P0.05),經(jīng)BMSCs治療后,治療組脾臟Foxp3 mRNA的表達(dá)水平高于肝硬化組(P0.05);肝硬化組大鼠脾臟CD4~+CD25~+Foxp3~+調(diào)節(jié)性T細(xì)胞百分比明顯低于正常組(P0.05),經(jīng)BMSCs治療后,治療組脾臟CD4~+CD25~+調(diào)節(jié)性T細(xì)胞百分比高于肝硬化組(P0.05)。結(jié)論 骨髓間充質(zhì)干細(xì)胞移植治療可通過(guò)抑制脾臟T、B淋巴細(xì)胞增殖,升高CD4~+CD25~+Foxp3~+調(diào)節(jié)性T細(xì)胞及Foxp3 mRNA表達(dá)水平,影響自身免疫功能,從而影響疾病的轉(zhuǎn)歸。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cell transplantation for the treatment effect on immune function in rats with liver cirrhosis, and provide a theoretical basis for clinical treatment. Methods 150 healthy male Wistar rats were randomly divided into 3 groups: control group (no intervention measures), liver cirrhosis group (for liver cirrhosis in rats preparation of the model), treatment group (bone marrow mesenchymal stem cell transplantation for treatment of liver cirrhosis model rats were given after preparation). Detection of spleen of T rats, B lymphocyte proliferation, CD4~+CD25~+Foxp3~+ regulatory T cells, mRNA expression level of Foxp3. The control structure of liver cells in rats, there is no fiber and pseudolobular proliferation; destruction of hepatic lobules of liver cirrhosis rats, pseudolobule hepatic cell edema, necrosis, inflammatory cells infiltration; experimental group rat liver cell edema degeneration and necrosis of liver cirrhosis group was significant Reduced, but still visible hyperplasia of fibrous tissue. The spleen T in cirrhotic rats, B lymphocyte proliferation was significantly higher than the control group (P0.05), after BMSCs treatment, treatment of spleen of rats in the T group, B lymphocyte proliferation was significantly lower than that in cirrhosis group (P0.05); the expression level of hepatic cirrhosis rats spleen Foxp3 mRNA was significantly lower than the control group (P0.05), after BMSCs treatment, the expression level of mRNA is higher than Foxp3 treatment group spleen liver cirrhosis group (P0.05); liver cirrhosis rats spleen CD4~+CD25~+Foxp3~+ percentage of T cells was significantly lower than the normal group (P0.05), after BMSCs treatment, treatment group spleen CD4~+CD25~+ the percentage of T cells higher than the cirrhosis group (P0.05). Conclusion: bone marrow mesenchymal stem cells transplantation through inhibition of spleen T, B lymphocyte proliferation, increased CD4~+CD25~+Foxp3~+ regulatory T cells and Foxp3 mRNA expression level, its influence The immune function, which affects the prognosis of the disease.

【作者單位】： 廣州衛(wèi)生職業(yè)技術(shù)學(xué)院檢驗(yàn)系;中山大學(xué)附屬腫瘤醫(yī)院生物治療中心;廣州市番禺區(qū)中心醫(yī)院皮膚科;
【分類號(hào)】：R-332;R575.2

【相似文獻(xiàn)】

相關(guān)會(huì)議論文 前2條

1 李靜;程留芳;;肝硬化大鼠模型腸上皮內(nèi)淋巴細(xì)胞的改變[A];中華醫(yī)學(xué)會(huì)第七次全國(guó)消化病學(xué)術(shù)會(huì)議論文匯編（下冊(cè)）[C];2007年

2 劉洪艷;王靜艷;劉沛;;肝硬化大鼠模型腦組織皮質(zhì)厚度與氨的關(guān)系[A];第五屆全國(guó)肝臟疾病臨床暨中華肝臟病雜志成立十周年學(xué)術(shù)會(huì)議論文匯編[C];2006年

相關(guān)博士學(xué)位論文 前1條

1 李靜;肝硬化大鼠模型腸道細(xì)菌移位和腸粘膜免疫屏障功能的研究[D];中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院;2007年

相關(guān)碩士學(xué)位論文 前2條

1 劉洪艷;氨對(duì)肝硬化大鼠模型腦神經(jīng)細(xì)胞凋亡的影響[D];中國(guó)醫(yī)科大學(xué);2003年

2 楊大偉;經(jīng)肝動(dòng)脈和門靜脈2種不同途徑移植骨髓間充質(zhì)干細(xì)胞對(duì)重癥肝硬化大鼠模型療效的對(duì)比[D];大連醫(yī)科大學(xué);2012年

，

本文編號(hào)：1569232