發(fā)布時間：2018-03-04 23:11

  本文選題：慢性高眼壓模型鼠　切入點：糖原合酶激酶β　出處：《眼科新進展》2017年04期 　論文類型：期刊論文

【摘要】：目的觀察慢性高眼壓模型鼠視網(wǎng)膜神經(jīng)節(jié)細胞(retina ganglion cells,RGCs)和視神經(jīng)中9位絲氨酸磷酸化糖原合酶激酶3β[p-GSK3β(ser9)]的表達變化,探討GSK3β是否在慢性高眼壓RGCs及視神經(jīng)退行性病變中發(fā)揮作用。方法取健康SD大鼠30只,隨機分為3組:模型對照組、慢性高眼壓模型2周組、慢性高眼壓模型4周組,每組10只。取大鼠右眼,采用鞏膜上靜脈結(jié)扎并燒灼法建立大鼠慢性高眼壓模型。分別于造模后2周和4周取5只大鼠眼球行冰凍切片,尼氏染色觀察RGCs數(shù)目,免疫熒光染色觀察RGCs和視神經(jīng)中p-GSK3β(ser9)的變化;各組取另外5只大鼠眼視網(wǎng)膜,Western blot檢測p-GSK3β(ser9)及總-GSK3β的表達。結(jié)果造模前3組大鼠右眼眼壓差異無統(tǒng)計學意義(P=0.89);造模后3組間眼壓差異有統(tǒng)計學意義(P0.01),慢性高眼壓模型2周組和4周組眼壓與模型對照組比較明顯升高,分別升高了59.13%和26.93%,差異均有統(tǒng)計學意義(均為P0.01)。視網(wǎng)膜尼氏染色RGCs計數(shù)發(fā)現(xiàn)模型對照組RGCs數(shù)為(149±12)個,慢性高眼壓模型2周組和4周組RGCs數(shù)較模型對照組明顯減少,分別為(120±10)個和(86±7)個,差異均有統(tǒng)計學意義(均為P0.05);且4周組比2周組進一步減少(P0.01)。慢性高眼壓模型2周組和4周組視網(wǎng)膜中p-GSK3β(ser9)陽性著色,同模型對照組相比,其RGCs層及視神經(jīng)中p-GSK3β(ser9)染色熒光減弱;4周組較模型對照組減弱更明顯。與模型對照組相比,慢性高眼壓模型2周組和4周組視網(wǎng)膜中總-GSK3β的表達差異無統(tǒng)計學意義(F=0.24,P=0.79);而3組間p-GSK3β(ser9)表達差異有統(tǒng)計學意義(P0.01),與模型對照組相比,慢性高眼壓模型2周組和4周組視網(wǎng)膜中p-GSK3β(ser9)分別減少了19.89%和36.46%(均為P0.05)。慢性高眼壓模型4周組比2周組視網(wǎng)膜中p-GSK3β(ser9)表達持續(xù)減少,差異具有統(tǒng)計學意義(P0.05)。結(jié)論慢性高眼壓模型鼠RGCs數(shù)目減少,RGCs和視神經(jīng)中p-GSK3β(ser9)表達減少,推測p-GSK3β(ser9)表達變化可能參與了慢性高眼壓模型鼠RGCs及視神經(jīng)退行性病變。
[Abstract]:Objective to observe the expression of serine phosphorylated glycogen kinase 3 尾 (p-GSK3 尾 serine kinase 3 尾) in retinal ganglion cells (RGCs) and optic nerve in rats with chronic intraocular hypertension (IOP). To investigate whether GSK3 尾 plays a role in chronic high intraocular pressure (RGCs) and optic neurodegenerative lesions, 30 healthy SD rats were randomly divided into three groups: model control group, chronic high intraocular pressure model group for 2 weeks and chronic high intraocular pressure model group for 4 weeks. Ten rats in each group were taken from the right eyes of the rats to establish the model of chronic high intraocular pressure by ligation and cauterization of the superior scleral vein. 5 rats' eyeballs were taken for frozen sections at 2 weeks and 4 weeks after the establishment of the model. The number of RGCs was observed by Nissl staining. The changes of RGCs and p-GSK3 尾 ser9 in optic nerve were observed by immunofluorescence staining. The expression of p-GSK3 尾 ser9) and total -GSK3 尾 in the retina of the other 5 rats were detected by Western blot. Results there was no significant difference in intraocular pressure between the three groups before modeling. The IOP in the 2 week and 4 week groups was significantly higher than that in the model control group. The number of RGCs in the model control group was 59.13% 鹵12, and the RGCs number in the chronic intraocular hypertension model group was significantly lower than that in the model control group at 2 weeks and 4 weeks, respectively (P 0.01), and the difference was statistically significant (P 0.01). The number of RGCs in the model control group was significantly lower than that in the control group, and the number of RGCs in the model group was significantly lower than that in the control group. The positive staining of p-GSK3 尾 ser9 in the retina of chronic intraocular hypertension model group 2 weeks and 4 weeks group was significantly lower than that of 2 week group, and the difference was statistically significant (P 0.05), and the positive staining of p-GSK3 尾 ser9 in the retina of chronic intraocular hypertension model group was significantly lower than that of 2 week group and 4 week group, compared with the model control group, and the expression of p-GSK3 尾 ser9 in the retina of the model group was significantly lower than that of the control group. The fluorescence of p-GSK3 尾 ser9 in the RGCs layer and optic nerve decreased more significantly in the 4-week group than in the model control group, and was significantly lower than that in the model control group. There was no significant difference in the expression of total -GSK3 尾 between two and four weeks of chronic intraocular pressure model, but there was no significant difference in the expression of p-GSK3 尾 ser9 between the three groups, compared with the model control group. The expression of p-GSK3 尾 ser9 in retina of chronic intraocular hypertension model decreased by 19.89% and 36.46 (both P0.05%, respectively). The expression of p-GSK3 尾 ser9 in retina of chronic intraocular hypertension model decreased continuously compared with that of 2-week group. Conclusion the number of RGCs and the expression of p-GSK3 尾 ser9 in the optic nerve of chronic intraocular hypertension model rats were decreased, which suggested that the changes of p-GSK3 尾 ser9) expression might be involved in RGCs and optic nerve degeneration in chronic high intraocular pressure model rats.
【作者單位】： 華中科技大學同濟醫(yī)學院附屬同濟醫(yī)院眼科;
【基金】：湖北省自然科學基金資助(編號:2014CFB442)~~
【分類號】：R-332;R774.1

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