本文關(guān)鍵詞： 丁基苯酞 阿爾茨海默病 學(xué)習(xí)記憶 Aβ-　出處：《西安交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年06期 　論文類型：期刊論文

【摘要】：目的觀察丁基苯酞對(duì)Aβ25-35致癡呆大鼠學(xué)習(xí)記憶的改善情況,并初步探索其作用機(jī)制。方法實(shí)驗(yàn)設(shè)立正常組、模型組和丁基苯酞組(10、30、100mg/kg),模型組和丁基苯酞組均采用側(cè)腦室注射Aβ25-35,干預(yù)組造模后給予丁基苯酞灌胃(灌胃劑量為2.5mL/kg)。利用水迷宮實(shí)驗(yàn)觀察大鼠的學(xué)習(xí)記憶能力,采用ATP檢測(cè)觀察腦組織內(nèi)線粒體功能的變化,并用試劑盒檢測(cè)線粒體相關(guān)酶活性的變化。結(jié)果水迷宮實(shí)驗(yàn)結(jié)果顯示,模型組大鼠學(xué)習(xí)記憶能力與正常組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),丁基苯酞10mg/kg組、30mg/kg組大鼠學(xué)習(xí)記憶障礙均有明顯改善(P0.05),100mg/kg組改變不明顯;線粒體相關(guān)檢測(cè)發(fā)現(xiàn),與正常組相比,模型組ATP水平明顯下降(P0.05),線粒體呼吸鏈復(fù)合物Ⅳ(P0.05)、丙酮酸脫氫酶復(fù)合體、α-酮戊二酸脫氫酶活性均明顯降低(P0.05)。與模型組相比,丁基苯酞能明顯提高線粒體相關(guān)酶的活性從而改善線粒體功能。結(jié)論丁基苯酞可以通過(guò)改善線粒體功能,提高Aβ25-35致癡呆大鼠的學(xué)習(xí)記憶能力。
[Abstract]:Objective to observe the effect of Ding Ji phthalide on learning and memory of dementia rats induced by A 尾 25-35, and to explore its mechanism. Model group and Ding Ji phthalide group (1030g 路kg ~ (-1) 路kg ~ (-1)), model group and Ding Ji phthalide group were treated with intracerebroventricular injection of A 尾 _ (25-35), then the intervention group was treated by intragastric instillation of Ding Ji phthalide at a dose of 2.5 mL / kg 路kg ~ (-1). The learning and memory abilities of rats were observed by water maze test. The changes of mitochondrial function in brain tissue were observed by ATP, and the activity of mitochondrial related enzymes was detected by kit. Results the results of water maze test showed that, There was significant difference in learning and memory ability between the model group and the normal group (P 0.05). The learning and memory impairment in the 10 mg / kg group of Ding Ji 10 mg / kg phthalide significantly improved the learning and memory impairment in the 100 mg / kg group, and the mitochondrial correlation test showed that compared with the normal group, the change of learning and memory impairment was not obvious. In the model group, the levels of ATP, the mitochondrial respiratory chain complex 鈪，

本文編號(hào)：1550086