本文關(guān)鍵詞： 大鼠 成纖維細(xì)胞生長(zhǎng)因子 骨髓間充質(zhì)干細(xì)胞 顱腦損傷　出處：《臨床和實(shí)驗(yàn)醫(yī)學(xué)雜志》2016年12期 　論文類(lèi)型：期刊論文

【摘要】：目的探討成纖維細(xì)胞生長(zhǎng)因子修飾的骨髓間充質(zhì)干細(xì)胞移植對(duì)顱腦損傷改善的機(jī)制。方法從大鼠中分離骨髓間充質(zhì)干細(xì)胞,培養(yǎng)、傳代,以腺病毒為載體介導(dǎo)成纖維細(xì)胞生長(zhǎng)因子轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞。以Feeney's法建立大鼠顱腦損傷模型60只,隨機(jī)分為對(duì)照組、骨髓間充質(zhì)干細(xì)胞組(BMSC組)和成纖維細(xì)胞生長(zhǎng)因子-骨髓間充質(zhì)干細(xì)胞組(FGF-modified BMSC組)。采用免疫組化法檢測(cè)Brd U標(biāo)記的骨髓間充質(zhì)干細(xì)胞在腦組織內(nèi)的分布,TUNEL法檢測(cè)腦組織細(xì)胞的凋亡情況。結(jié)果培養(yǎng)骨髓間充質(zhì)干細(xì)胞,Western-Blot法顯示成纖維細(xì)胞生長(zhǎng)因子成功轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞。成纖維細(xì)胞生長(zhǎng)因子-骨髓間充質(zhì)干細(xì)胞組的Brd U標(biāo)記陽(yáng)性細(xì)胞數(shù)顯著性高于骨髓間充質(zhì)干細(xì)胞組(P0.05),而凋亡性細(xì)胞個(gè)數(shù)明顯減少(P0.05)。移植14 d后,成纖維細(xì)胞生長(zhǎng)因子-骨髓間充質(zhì)干細(xì)胞大鼠的Longa神經(jīng)功能評(píng)分顯著性低于對(duì)照組和骨髓間充質(zhì)干細(xì)胞組(P0.05)。結(jié)論骨髓間充質(zhì)干細(xì)胞能很好地存在于受損的大鼠腦內(nèi),并能夠減少受損組織細(xì)胞的凋亡,從而改善顱腦損傷大鼠的神經(jīng)功能損傷。
[Abstract]:Objective to investigate the mechanism of bone marrow mesenchymal stem cell transplantation modified by fibroblast growth factor (FGF) on the improvement of craniocerebral injury. Methods Bone marrow mesenchymal stem cells (BMSCs) were isolated from rats, cultured and subcultured. Bone marrow mesenchymal stem cells (BMSCs) were transfected with adenovirus mediated fibroblast growth factor (FGF). Sixty rat models of craniocerebral injury were established by Feeney's method and were randomly divided into control group. Bone marrow mesenchymal stem cells (BMSCs) and fibroblast growth factor-bone marrow mesenchymal stem cells (FGF-modified BMSC) were used to detect the distribution of bone marrow mesenchymal stem cells (BMSCs) labeled with Brd U in brain tissue by immunohistochemistry and Tunel method. Results Western-Blot method showed that fibroblast growth factor was successfully transfected into bone marrow mesenchymal stem cells. Brd of fibroblast growth factor-bone marrow mesenchymal stem cells group. The number of U labeled positive cells was significantly higher than that of bone marrow mesenchymal stem cells (BMSCs), while the number of apoptotic cells decreased significantly. The Longa neural function score of fibroblast growth factor-bone marrow mesenchymal stem cell rats was significantly lower than that of control group and bone marrow mesenchymal stem cell group (P 0.05). Conclusion Bone marrow mesenchymal stem cells can well exist in the damaged rat brain. It can reduce the apoptosis of injured tissue cells and improve the nerve function injury of rats with craniocerebral injury.
【作者單位】： 上海交通大學(xué)附屬第六人民醫(yī)院神經(jīng)外科;
【基金】：上海市科學(xué)技術(shù)委員會(huì)科研計(jì)劃項(xiàng)目(編號(hào):13411951401)
【分類(lèi)號(hào)】：R651.15;R-332

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