本文關(guān)鍵詞： 脾虛證 白色念珠菌 T細(xì)胞亞群 穿孔素 顆粒酶B　出處：《中華中醫(yī)藥學(xué)刊》2017年08期 　論文類型：期刊論文

【摘要】：目的:通過觀察腸道白色念珠菌感染對(duì)脾虛小鼠小腸黏膜固有層中T細(xì)胞亞群和穿孔素、顆粒酶的影響,探討機(jī)體在脾虛狀態(tài)下感染白色念珠菌的部分免疫機(jī)制。方法:本實(shí)驗(yàn)采用飲食失節(jié)加勞倦過度的復(fù)合因素制備小鼠脾虛模型,經(jīng)腸道感染白色念珠菌后第14天,應(yīng)用流式細(xì)胞術(shù)檢測(cè)小鼠小腸黏膜固有層中CD_3~+、CD_4~+和CD_8~+T的細(xì)胞百分比,采用免疫熒光法檢測(cè)各組小鼠小腸黏膜中穿孔素、顆粒酶陽性表達(dá)細(xì)胞數(shù)。結(jié)果:與正常對(duì)照組比較,其他3組小鼠小腸黏膜固有層中CD_4~+T細(xì)胞比例均有不同程度下降,CD_8~+T細(xì)胞比例均有不同程度上升,有統(tǒng)計(jì)學(xué)意義(P0.01);正常感染白念組和脾虛感染白念組小鼠小腸黏膜固有層中穿孔素陽性表達(dá)細(xì)胞均明顯增多(P0.05或P0.01),而顆粒酶陽性表達(dá)細(xì)胞僅脾虛感染白念組明顯增多(P0.01)。其中,脾虛感染白念組最顯著,與其他3組均具有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。結(jié)論:小腸黏膜固有層中穿孔素和顆粒酶的高表達(dá)可能是造成小腸局部損傷的免疫機(jī)制之一,脾虛小鼠腸道局部免疫功能下降,感染發(fā)生后可加重腸道局部損傷程度。
[Abstract]:Objective: to observe the effect of Candida albicans infection on T cell subsets, perforin and granzyme in lamina propria of spleen deficiency mice. To explore the partial immune mechanism of Candida albicans infection in spleen deficiency. Methods: the spleen deficiency model of mice was established by the compound factors of diet loss and overwork. The mice were infected with Candida albicans on the 14th day after intestinal infection. Flow cytometry was used to detect the percentage of CD3 ~ + CD4 ~ and CD8 ~ T in the lamina propria of small intestine of mice, and immunofluorescence assay was used to detect the number of perforin and granzyme positive cells in the intestinal mucosa of mice. The percentage of CD4 ~ T cells in the lamina propria of the other three groups decreased in varying degrees, and the percentage of CD8 ~ T cells increased in different degrees. The positive expression of perforin in the lamina propria of small intestine of normal infection group and spleen deficiency group were significantly increased (P 0.05 or P 0.01), while the expression of granzyme positive cells was significantly increased only in the group of spleen deficiency infection (P 0.01), and the expression of perforin in the lamina propria of small intestine of mice infected with spleen deficiency was significantly higher than that in group B (P 0.01). Conclusion: the high expression of perforin and granzyme in the lamina propria of small intestine may be one of the immune mechanisms of local injury of small intestine. The local immune function of spleen deficiency mice decreased, and the degree of local intestinal injury was aggravated after infection.
【作者單位】： 遼寧中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金項(xiàng)目(81173145,81403208) 遼寧中醫(yī)藥大學(xué)省部共建中醫(yī)臟象理論及應(yīng)用教育部重點(diǎn)實(shí)驗(yàn)室項(xiàng)目
【分類號(hào)】：R259;R-332
，

本文編號(hào)：1519572