本文關(guān)鍵詞： 結(jié)核分枝桿菌 Rv 抗原性 巨噬細(xì)胞 凋亡　出處：《中國(guó)免疫學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的:研究結(jié)核分枝桿菌Rv3425蛋白免疫學(xué)特性,評(píng)估其診斷應(yīng)用價(jià)值及在結(jié)核致病性中的作用。方法:將Rv3425基因克隆至p ET28a載體中,誘導(dǎo)表達(dá)Rv3425融合蛋白并進(jìn)行純化;利用ELISA、Western blot分析其抗原性與特異性;流式檢測(cè)該抗原對(duì)巨噬細(xì)胞凋亡的影響。結(jié)果:獲得了可溶性原核表達(dá)融合蛋白R(shí)v3425,Rv3425蛋白能刺激滅活結(jié)核分枝桿菌免疫小鼠脾細(xì)胞產(chǎn)生高水平的特異性IFN-γ、純化的Rv3425蛋白能與結(jié)核感染小鼠血清特異性結(jié)合,其特異性血清抗體Ig G及Ig M在結(jié)核病人中的水平明顯高于健康人,發(fā)現(xiàn)該蛋白能誘導(dǎo)巨噬細(xì)胞的凋亡。結(jié)論:本研究發(fā)現(xiàn)結(jié)核分枝桿菌Rv3425蛋白具有較強(qiáng)的抗原性且能促進(jìn)巨噬細(xì)胞的凋亡,這些發(fā)現(xiàn)對(duì)于結(jié)核病的診斷及致病機(jī)制的研究具有重要價(jià)值。
[Abstract]:Objective: to study the immunological characteristics of Rv3425 protein of Mycobacterium tuberculosis and to evaluate its diagnostic value and its role in the pathogenicity of tuberculosis. Methods: the Rv3425 gene was cloned into p ET28a vector to express and purify the Rv3425 fusion protein. The antigenicity and specificity of EISAL were analyzed by Western blot. Results: the soluble prokaryotic expression fusion protein Rv3425rv3425 could stimulate the production of high specific IFN- 緯 by inactivated Mycobacterium tuberculosis immunized mice spleen cells and purified Rv3425. The protein can specifically bind to the serum of mice infected with tuberculosis. The levels of IgG and IgM in tuberculosis patients were significantly higher than those in healthy persons. Conclusion: the Rv3425 protein of Mycobacterium tuberculosis has strong antigenicity and can promote the apoptosis of macrophages. These findings are of great value in the diagnosis and pathogenesis of tuberculosis.
【作者單位】： 天津市海河醫(yī)院病理科 國(guó)家中醫(yī)藥管理局中醫(yī)藥防治傳染病重點(diǎn)研究室;天津醫(yī)科大學(xué)總醫(yī)院檢驗(yàn)科;武漢大學(xué)基礎(chǔ)醫(yī)學(xué)院;武漢大學(xué)中南醫(yī)院感染科;
【基金】：2014年湖北省科技廳面上項(xiàng)目(2014CFB392)
【分類號(hào)】：R378.911
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本文編號(hào)：1509333