本文關(guān)鍵詞： Echo 型病毒 病毒性腦炎 VP 分子流行病學(xué)　出處：《病毒學(xué)報(bào)》2016年06期 　論文類型：期刊論文

【摘要】：了解中國福建省龍巖市2011~2014年病毒性腦炎散發(fā)病例中Echo 30分子流行病學(xué)特征。收集病毒性腦炎或中樞神經(jīng)系統(tǒng)感染腦脊液標(biāo)本,細(xì)胞培養(yǎng)分離病毒,RT-PCR擴(kuò)增VP1基因序列全長以鑒定Echo 30血清型及遺傳特征分析。2011~2014年共從608例監(jiān)測病例中分離到168株腸道病毒,其中60株為Echo 30;60例Echo 30相關(guān)病例分析顯示,Echo 30流行高峰為6~8月;波及年齡范圍廣,以小于10歲高發(fā)(65%);臨床癥狀以發(fā)熱、頭痛和嘔吐為主,腦脊液清,細(xì)胞和蛋白含量檢測多增加。VP1區(qū)分析顯示,2011~2014年龍巖流行株均屬于h基因型,但有兩個(gè)傳播鏈共同循環(huán);與2011年導(dǎo)致福建省病毒性腦炎暴發(fā)毒株高度同源,但2014年龍巖株均在VP1蛋白I 120V出現(xiàn)新氨基酸變異。福建省2011年暴發(fā)流行株仍流行于龍巖市,且兩個(gè)傳播鏈病毒仍共同循環(huán),但2014年病毒株出現(xiàn)了新的變異,持續(xù)監(jiān)測將有利于早期發(fā)現(xiàn)病毒變異積累和評估疾病流行的風(fēng)險(xiǎn)。
[Abstract]:To investigate the molecular epidemiological characteristics of Echo 30 in sporadic cases of viral encephalitis from 2011 to 2014 in Longyan City, Fujian Province, China. The cerebrospinal fluid (CSF) samples of viral encephalitis or central nervous system infection were collected. The virus was isolated by cell culture. Amplification of full length VP1 Gene sequence by RT-PCR to identify Echo. Analysis of 30 serotypes and genetic characteristics. 168 strains of enterovirus were isolated from 608 surveillance cases from 2011 to 2014. Among them, 60 strains were Echo 30; The analysis of 60 Echo 30 related cases showed that the peak of Echo 30 epidemic was from 6 to August. The affected age was wide, with a high incidence of 65% less than 10 years old; The clinical symptoms were fever, headache and vomiting. The detection of cerebrospinal fluid (CSF), cell and protein increased. The analysis of VP1 region showed that Longyan epidemic strain belonged to h genotype from 2011 to 2014. However, there are two transmission chains in common circulation; It is highly homologous to the virus encephalitis outbreak in Fujian province in 2011. In 2014, the new amino acid variation of Longyan strain was found in VP1 protein I120V. the outbreak in Fujian Province in 2011 was still prevalent in Longyan City, and the two transmission chain viruses were still circulating together. In 2014, however, a new mutation appeared in the virus strain, and continuous monitoring will facilitate early detection of the accumulation of variation and assessment of the risk of disease prevalence.
【作者單位】： 龍巖市疾病預(yù)防控制中心;福建醫(yī)科大學(xué)龍巖市疾病預(yù)防控制中心教學(xué)點(diǎn);福建省疾病預(yù)防控制中心;
【基金】：福建省衛(wèi)生廳青年科研課題(項(xiàng)目號:2012-2-126),題目:病毒性腦炎與腸道病毒感染關(guān)系研究
【分類號】：R373
【正文快照】： 埃可病毒(Echovirus,Echo)可引起腦膜炎、腦炎、心肌炎及新生兒嚴(yán)重疾病,于1950年初次分離,屬B組腸道病毒(Enterovirus,EV),有許多血清型,Echo 30是腸道病毒性腦炎相關(guān)病例常見病原體之一。檢測發(fā)現(xiàn)龍巖市曾分別發(fā)生過由Echo 19型和Echo 6型引起的病毒性腦炎流行[1-2],在2004~，

本文編號：1478978