本文關(guān)鍵詞： 短串聯(lián)重復(fù)序列 群體遺傳學(xué) 中國(guó)南方漢族　出處：《南方醫(yī)科大學(xué)學(xué)報(bào)》2017年02期 　論文類(lèi)型：期刊論文

【摘要】：目的評(píng)估Power Plex~?21系統(tǒng)所含基因座的等位基因頻率、法醫(yī)學(xué)常用遺傳學(xué)參數(shù)和系統(tǒng)效能。方法對(duì)Power Plex~?21系統(tǒng)包含的20個(gè)常染色體STR基因座(D3S1358、D1S1656、D6S1043、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433、FGA)進(jìn)行基因分型。統(tǒng)計(jì)2367個(gè)南方漢族無(wú)關(guān)個(gè)體中上述20個(gè)基因座的等位基因頻率和遺傳學(xué)參數(shù),并將本研究人群的等位基因頻率與文獻(xiàn)報(bào)道的其他人群數(shù)據(jù)進(jìn)行比較。結(jié)果該系統(tǒng)各基因座的個(gè)體識(shí)別率為0.7839~0.9852,非父排除率為0.2974~0.8099。除D5S818基因座外,其余基因座均符合哈-溫平衡。該系統(tǒng)的累積非父排除率和累積個(gè)體識(shí)別率均超過(guò)0.999 999 999 999 999 999 999 999999 999。南方漢族人群與8個(gè)少數(shù)民族(廣西彝族、廣西回族、廣西苗族、貴州侗族、貴州苗族、青海土家族、青�；刈�、云南彝族)相比,等位基因頻率有顯著性差異。中國(guó)南方漢族人群和日本、菲律賓、韓國(guó)、北意大利和阿根廷等人群相比,在8-20個(gè)STR基因座上存在顯著性差異。南方漢族人群與上述5組國(guó)外人群以及及3組文獻(xiàn)報(bào)道的國(guó)內(nèi)人群(北京漢族、浙江漢族、福建漢族)在D1S1656基因座上的等位基因頻率均有顯著性差異。Neighbor-joining系統(tǒng)發(fā)生樹(shù)顯示所有亞洲人群聚類(lèi)為一個(gè)分支,意大利北部和阿根廷人群聚集成另一個(gè)獨(dú)立的分支,南方漢族人群和云南彝族人群遺傳距離較近。結(jié)論 Power Plex?21系統(tǒng)的20個(gè)STR基因座在中國(guó)南方漢族人群具有較高的遺傳多態(tài)性,可滿(mǎn)足親子鑒定、個(gè)體識(shí)別以及人類(lèi)學(xué)研究的需求。
[Abstract]:Objective to evaluate Power Plexus. The allelic frequency of the loci contained in system 21, the common genetic parameters of forensic medicine and system effectiveness. System 21 contains 20 autosomal STR loci D3S1358D1S1656, D6S1043, D13S317penta EOD16S539. D18S51,D2S1338,CSF1PO,Penta D,TH01,vWA,D21S11,D7S820,D5S818,TPOX. D8S1179,D12S391,D19S433. The allele frequencies and genetic parameters of the above 20 loci in 2367 unrelated southern Han individuals were analyzed. The allele frequency of the study population was compared with other population data reported in the literature. Results the individual recognition rate of each locus of the system was 0.7839 / 0.9852. The non-paternal exclusion rate was 0.2974 and 0.8099. except for D5S818 locus. All the other loci were in the Hard-temperature equilibrium. The cumulative non-paternal exclusion rate and cumulative individual identification rate of the system were over 0.999 999 999 999 respectively. 999999. Han nationality and 8 ethnic minorities in southern China (. Guangxi Yi nationality. There were significant differences in allele frequencies among Hui, Miao, Dong, Miao, Tujia, Tujia, Hui and Yi in Yunnan. South Korea, northern Italy and Argentina were compared. There were significant differences among 8 to 20 STR loci. There were significant differences between the southern Han population and the foreign population mentioned above, as well as the domestic population reported in 3 groups (Han nationality in Beijing and Han nationality in Zhejiang province). The allelic frequencies of D1S1656 loci in Fujian Han nationality were significantly different. The phylogenetic tree of the Neighbor-Joining system showed that all Asian populations were clustered into one branch. The population of northern Italy and Argentina gathered into another independent branch. The genetic distance between the Han population of the south and the Yi people of Yunnan was close. Conclusion Power Plexx? The 20 STR loci of the 21 system have high genetic polymorphisms in the Han population of southern China, which can meet the needs of paternity identification, individual identification and anthropology.
【作者單位】： 南方醫(yī)科大學(xué)法醫(yī)學(xué)院;廣州市刑事科學(xué)技術(shù)研究所;
【基金】：Supported by Natural Science Foundation of Guangdong Province(2014A030310025) Open Project of Key Laboratory of Forensic Genetics in Ministry of Public Security(2015FGKFKT03) Medical Science and Technology Research Foundation of Guangdong Province(A2015043)~~
【分類(lèi)號(hào)】：R394
【正文快照】： INTRODUCTION The Power Plex?21 System is a widely used commercial kit for short-tandem repeat(STR)genotyping[1-3],which yields reliable results with a robust design[4].Developed by Promega Corporation in 2012,this system has been shown to have a good in

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