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  本文關鍵詞： 短串聯(lián)重復序列 群體遺傳學 中國南方漢族　出處：《南方醫(yī)科大學學報》2017年02期 　論文類型：期刊論文

【摘要】：目的評估Power Plex~?21系統(tǒng)所含基因座的等位基因頻率、法醫(yī)學常用遺傳學參數(shù)和系統(tǒng)效能。方法對Power Plex~?21系統(tǒng)包含的20個常染色體STR基因座(D3S1358、D1S1656、D6S1043、D13S317、Penta E、D16S539、D18S51、D2S1338、CSF1PO、Penta D、TH01、vWA、D21S11、D7S820、D5S818、TPOX、D8S1179、D12S391、D19S433、FGA)進行基因分型。統(tǒng)計2367個南方漢族無關個體中上述20個基因座的等位基因頻率和遺傳學參數(shù),并將本研究人群的等位基因頻率與文獻報道的其他人群數(shù)據(jù)進行比較。結果該系統(tǒng)各基因座的個體識別率為0.7839~0.9852,非父排除率為0.2974~0.8099。除D5S818基因座外,其余基因座均符合哈-溫平衡。該系統(tǒng)的累積非父排除率和累積個體識別率均超過0.999 999 999 999 999 999 999 999999 999。南方漢族人群與8個少數(shù)民族(廣西彝族、廣西回族、廣西苗族、貴州侗族、貴州苗族、青海土家族、青�；刈�、云南彝族)相比,等位基因頻率有顯著性差異。中國南方漢族人群和日本、菲律賓、韓國、北意大利和阿根廷等人群相比,在8-20個STR基因座上存在顯著性差異。南方漢族人群與上述5組國外人群以及及3組文獻報道的國內人群(北京漢族、浙江漢族、福建漢族)在D1S1656基因座上的等位基因頻率均有顯著性差異。Neighbor-joining系統(tǒng)發(fā)生樹顯示所有亞洲人群聚類為一個分支,意大利北部和阿根廷人群聚集成另一個獨立的分支,南方漢族人群和云南彝族人群遺傳距離較近。結論 Power Plex?21系統(tǒng)的20個STR基因座在中國南方漢族人群具有較高的遺傳多態(tài)性,可滿足親子鑒定、個體識別以及人類學研究的需求。
[Abstract]:Objective to evaluate Power Plexus. The allelic frequency of the loci contained in system 21, the common genetic parameters of forensic medicine and system effectiveness. System 21 contains 20 autosomal STR loci D3S1358D1S1656, D6S1043, D13S317penta EOD16S539. D18S51,D2S1338,CSF1PO,Penta D,TH01,vWA,D21S11,D7S820,D5S818,TPOX. D8S1179,D12S391,D19S433. The allele frequencies and genetic parameters of the above 20 loci in 2367 unrelated southern Han individuals were analyzed. The allele frequency of the study population was compared with other population data reported in the literature. Results the individual recognition rate of each locus of the system was 0.7839 / 0.9852. The non-paternal exclusion rate was 0.2974 and 0.8099. except for D5S818 locus. All the other loci were in the Hard-temperature equilibrium. The cumulative non-paternal exclusion rate and cumulative individual identification rate of the system were over 0.999 999 999 999 respectively. 999999. Han nationality and 8 ethnic minorities in southern China (. Guangxi Yi nationality. There were significant differences in allele frequencies among Hui, Miao, Dong, Miao, Tujia, Tujia, Hui and Yi in Yunnan. South Korea, northern Italy and Argentina were compared. There were significant differences among 8 to 20 STR loci. There were significant differences between the southern Han population and the foreign population mentioned above, as well as the domestic population reported in 3 groups (Han nationality in Beijing and Han nationality in Zhejiang province). The allelic frequencies of D1S1656 loci in Fujian Han nationality were significantly different. The phylogenetic tree of the Neighbor-Joining system showed that all Asian populations were clustered into one branch. The population of northern Italy and Argentina gathered into another independent branch. The genetic distance between the Han population of the south and the Yi people of Yunnan was close. Conclusion Power Plexx? The 20 STR loci of the 21 system have high genetic polymorphisms in the Han population of southern China, which can meet the needs of paternity identification, individual identification and anthropology.
【作者單位】： 南方醫(yī)科大學法醫(yī)學院;廣州市刑事科學技術研究所;
【基金】：Supported by Natural Science Foundation of Guangdong Province(2014A030310025) Open Project of Key Laboratory of Forensic Genetics in Ministry of Public Security(2015FGKFKT03) Medical Science and Technology Research Foundation of Guangdong Province(A2015043)~~
【分類號】：R394
【正文快照】： INTRODUCTION The Power Plex?21 System is a widely used commercial kit for short-tandem repeat(STR)genotyping[1-3],which yields reliable results with a robust design[4].Developed by Promega Corporation in 2012,this system has been shown to have a good in

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