本文關(guān)鍵詞： 香鱗毛蕨有效部位乳膏 體內(nèi)抗真菌活性 糠秕馬拉色菌 豚鼠模型　出處：《中國(guó)實(shí)驗(yàn)方劑學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:評(píng)價(jià)香鱗毛蕨有效部位乳膏(DF乳膏)對(duì)糠秕馬拉色菌所致豚鼠感染模型的治療作用。方法:構(gòu)建糠秕馬拉色菌豚鼠皮膚感染模型,按皮損評(píng)分值隨機(jī)分成模型組,酮康唑組(1.33 mg·g-1),DF乳膏高、中、低劑量組(含生藥2,1,0.5 g·g-1),通過(guò)測(cè)定肉眼評(píng)分、菌落計(jì)數(shù)(CFU),真菌學(xué)轉(zhuǎn)陰率及組織病理檢查4項(xiàng)指標(biāo),評(píng)價(jià)DF乳膏對(duì)糠秕馬拉色菌所致豚鼠感染模型的治療作用。結(jié)果:與模型組比較,DF乳膏高、中、低劑量組均可顯著降低糠秕馬拉色菌所致感染模型豚鼠皮損評(píng)分(P0.05),明顯減少病變皮膚菌落形成數(shù)(P0.05),提高真菌培養(yǎng)的轉(zhuǎn)陰率(P0.05,P0.01);皮膚病理切片結(jié)果表明DF乳膏高、中劑量可明顯減少感染組織皮膚角質(zhì)層孢子數(shù)。結(jié)論:DF乳膏對(duì)糠秕馬拉色菌所致的豚鼠感染有較好的治療作用。
[Abstract]:Objective: to evaluate the therapeutic effect of extract DF cream of Dryopteris furfurum on guinea pig infected with Malassezia furfurum. Methods: the skin infection model of Malassezia furfurum in guinea pigs was established. According to the score of skin lesion, ketoconazole group was randomly divided into three groups: the ketoconazole group was 1.33 mg 路g-1D ointment with high, middle and low doses (containing 21g 路g ~ (-1)) crude drug. Four indexes including gross score, colony count, mycological negative rate and histopathological examination were measured. To evaluate the therapeutic effect of DF cream on guinea pig infection induced by Malassezia furfurum. Results: compared with the model group, DF cream was higher and higher than that of the model group. The low dose group could significantly reduce the skin lesion score of guinea pigs induced by Malassezia furfurum (P0.05), and significantly reduce the number of diseased skin colony formation (P0.05). To increase the negative rate of fungi culture. The results of pathological section of skin showed that DF cream was high and medium dose could obviously reduce the number of spores in the cuticle of infected tissues. Conclusion the solution of 10% DF cream has a better therapeutic effect on the infection caused by Malassezia furfurum in guinea pigs.
【作者單位】： 廣東藥科大學(xué)中藥學(xué)院實(shí)驗(yàn)動(dòng)物中心;
【基金】：國(guó)家中醫(yī)藥管理局公益性行業(yè)科研專項(xiàng)(201507004) 國(guó)家“重大新藥創(chuàng)制”科技重大專項(xiàng)(2011ZX09102-007-03) 廣東省科技計(jì)劃項(xiàng)目(2015B020234009) 廣東省公益研究與能力建設(shè)專項(xiàng)資金項(xiàng)目(2015A030302088)
【分類號(hào)】：R275;R-332
【正文快照】： [網(wǎng)絡(luò)出版地址]http://www.cnki.net/kcms/detail/11.3495.R.20161129.1058.018.html[網(wǎng)絡(luò)出版時(shí)間]2016-11-29 10:58糠秕馬拉色菌Malassezia furfur,屬于馬拉色菌屬中常見(jiàn)的皮膚寄生菌致病菌。從馬拉色菌屬所致的各類型疾病中均能分離出M.furfur[1],該菌主要分布于人的頭皮、

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