本文關(guān)鍵詞： 人心肌細(xì)胞 脂聯(lián)素 氧化應(yīng)激 細(xì)胞凋亡　出處：《山西醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：研究在高糖環(huán)境下及加入脂聯(lián)素(Adiponectin, ADPN)后對人心肌細(xì)胞增殖、凋亡情況及氧化應(yīng)激指標(biāo)ROS的影響,揭示脂聯(lián)素在糖尿病心肌病中保護(hù)心肌細(xì)胞的作用。 方法：將培養(yǎng)的人心肌細(xì)胞分為：正常對照組(N組)、高糖組(G組)、高糖+脂聯(lián)素組(A組),分別培養(yǎng)24h,48h及72h,用MTT法(四甲基偶氮唑藍(lán))檢測細(xì)胞增殖,采用Western blotting法檢測各組細(xì)胞凋亡相關(guān)蛋白Bax, Bcl-2的表達(dá),用熒光探針DCFH-DA檢測心肌細(xì)胞內(nèi)活性氧(ROS)水平,觀察細(xì)胞凋亡。 結(jié)果：：①MTT法檢測人心肌細(xì)胞增殖情況,24h高糖組與高糖+脂聯(lián)素組比較差異無統(tǒng)計學(xué)意義,48h和72h高糖組與對照組相比增殖明顯減弱(p0.05),并且高糖+脂聯(lián)素組細(xì)胞增殖較高糖組明顯增加,差異有統(tǒng)計學(xué)意義(p0.05)。②高糖組較對照組中人心肌細(xì)胞bcl-2蛋白含量下降,Bax蛋白含量上升,加入脂聯(lián)素后,bcl-2蛋白含量顯著上升,Bax蛋白含量顯著下降,差異有統(tǒng)計學(xué)意義(p0.05)。③ROS在高糖組表達(dá)較正常組升高,脂聯(lián)素干預(yù)后,ROS表達(dá)水平下降接近對照組,差異有統(tǒng)計學(xué)意義(p0.05)。 結(jié)論：高糖的持續(xù)作用可以抑制人心肌細(xì)胞的增殖,增加人心肌細(xì)胞的氧化應(yīng)激損傷；加入脂聯(lián)素干預(yù)后,細(xì)胞的增殖活性增加,減少了對人心肌細(xì)胞的氧化應(yīng)激損傷,能保護(hù)高糖環(huán)境下人心肌細(xì)胞。
[Abstract]:Objective: To study the effects of Adiponectin (ADPN) on the proliferation, apoptosis and oxidative stress index ROS of human cardiac myocytes, and to reveal the role of adiponectin in protecting cardiomyocytes from diabetic cardiomyopathy.
Methods: the cultured myocardial cells were divided into normal control group (N group), high glucose group (G group), high glucose + adiponectin group (A group), 48h and 72h were cultured in 24h, using the MTT method (four methyl thiazolyl tetrazolium) cell proliferation assay, using Western blotting method to detect the fine apoptosis related protein Bax, Bcl-2 expression in myocardial cells of DCFH-DA fluorescent probe for detection of reactive oxygen species (ROS) level, the cell apoptosis was observed.
Results: the detection of human myocardial cell proliferation MTT assay, 24h high glucose group and high glucose + adiponectin group had no significant difference, 48h and 72h in high glucose group compared with the control group, the proliferation was significantly decreased (P0.05), and the proliferation of high glucose + adiponectin group were significantly increased compared with the high glucose group, the difference was statistically significant (P0.05). The high glucose group was lower than that of control group in the myocardial cell Bcl-2 protein, Bax protein content increased, adding adiponectin, significantly increased the content of Bcl-2 protein, Bax protein content decreased significantly, the difference was statistically significant (P0.05). The expression of ROS in high glucose group than in the normal group, adiponectin intervention, the expression level of ROS decreased to the control group, the difference was statistically significant (P0.05).
Conclusion: the effect of high glucose can inhibit myocardial cell proliferation, increase the damage of oxidative stress; adding adiponectin intervention, cell proliferation activity increased, reducing the oxidative stress injury of myocardial cells and protects human myocardial cells in high glucose environment.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 董矜,田亞平;一氧化氮代謝異常和糖尿病[J];軍醫(yī)進(jìn)修學(xué)院學(xué)報;2004年01期

2 趙宏宇;伊桐凝;張錦;;球狀脂聯(lián)素抑制波動性高血糖誘導(dǎo)人臍靜脈內(nèi)皮細(xì)胞凋亡與脂聯(lián)素受體1有關(guān)[J];中國動脈硬化雜志;2010年07期

3 張媛;李英敏;馮月秋;常彩云;潘華偉;王束玫;;血清脂聯(lián)素水平與肥胖、胰島素抵抗的關(guān)系探討[J];山東大學(xué)學(xué)報(醫(yī)學(xué)版);2009年06期

4 李興;張木勛;;Expression of c-met Stimulated by High Glucose in Human Renal Tubular Epithelial Cells and Its Implication[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2007年02期

5 吳強(qiáng);楊永曜;李隆貴;楊天和;蔡運昌;蔣清安;;非諾貝特和吡格列酮對血管緊張素Ⅱ介導(dǎo)的心肌細(xì)胞肥大和凋亡的干預(yù)作用[J];中華高血壓雜志;2007年03期

6 張薇;鐘明;唐夢熊;馬驍;苗雅;孫慧;張運;;Tribble 3在糖尿病心肌病心肌間質(zhì)重構(gòu)時的表達(dá)和纈沙坦干預(yù)的影響[J];中華心血管病雜志;2006年03期

，

本文編號：1460934