本文關(guān)鍵詞： 玄參 脾虛水濕不化 代謝組學(xué) 生物標(biāo)志物 代謝通路 黃嘌呤 磷脂酰乙醇胺　出處：《中國(guó)實(shí)驗(yàn)方劑學(xué)雜志》2017年20期 　論文類型：期刊論文

【摘要】：目的:探索玄參對(duì)脾虛水濕不化大鼠模型的影響,并利用代謝組學(xué)方法對(duì)生物標(biāo)志物和代謝通路進(jìn)行分析。方法:高脂低蛋白飼料喂養(yǎng)加尾部負(fù)重游泳的方法建立脾虛水濕不化大鼠模型,玄參水煎液高、低劑量給藥2周后檢測(cè)各給藥組肝臟指數(shù)、血清蛋白、血脂、胃腸功能和水負(fù)荷的變化情況,給藥劑量分別為1.35,5.40 g·kg-1。利用UPLC-Q-TOF-MS檢測(cè)肝組織中內(nèi)源性小分子代謝物,電噴霧正離子模式(ESI+)和負(fù)離子模式(ESI-)對(duì)樣品進(jìn)行檢測(cè),m/z 100~1 500;利用主成分分析(PCA)和偏最小二乘法-判別分析(PLS-DA)比較空白組、模型組,玄參高、低劑量組內(nèi)源性小分子代謝物的變化情況;對(duì)差異代謝物進(jìn)行京都基因與基因組百科全書(KEGG)代謝通路分析。結(jié)果:與模型組比較,玄參高劑量組的胃排空率和D-木糖排泄率顯著升高;玄參低劑量組能升高血清白蛋白、總蛋白含量;玄參高劑量組能降低總膽固醇、低密度脂蛋白膽固醇含量和水負(fù)荷指數(shù)。代謝組學(xué)方法共鑒定出21個(gè)生物標(biāo)志物,找到7條主要代謝通路,主要涉及胰島素分泌、甘油磷脂代謝、嘌呤代謝、膽汁酸分泌、磷脂酶D信號(hào)通路、糖基磷脂酰肌醇錨的合成、內(nèi)源性大麻素的生物合成等。結(jié)論:玄參對(duì)脾虛水濕不化模型大鼠的影響與改善消化功能、抑制糖代謝、改善血脂代謝等有關(guān)。
[Abstract]:Objective: to explore the effect of Xuanshen on the rat model of spleen deficiency and dampness. The biomarkers and metabolic pathways were analyzed by the method of metabolomics. Methods: high fat and low protein feed plus tail weight bearing swimming method was used to establish the model of spleen deficiency water dampness in rats, and the decoction of Radix Sophora sinensis was high. The changes of liver index, serum protein, blood lipid, gastrointestinal function and water load in each group were measured 2 weeks after low dose administration, the dosage was 1.35 respectively. 5.40g 路kg-1.The endogenous small molecule metabolites in liver tissue were detected by UPLC-Q-TOF-MS. Electrospray positive ion mode (ESI) and negative ion mode (ESI) were used to detect the samples. The changes of endogenous small molecule metabolites in blank group, model group, high and low dose group were compared by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DAA). Results: compared with the model group, the gastric emptying rate and the D-xylose excretion rate of the high dose group were significantly higher than those of the model group. The contents of serum albumin and total protein were increased in the low dose group. High dose group could reduce total cholesterol, low density lipoprotein cholesterol content and water load index. A total of 21 biomarkers were identified and 7 main metabolic pathways were found. Mainly involved in insulin secretion, glycerol phospholipid metabolism, purine metabolism, bile acid secretion, phospholipase D signaling pathway, glycosylphosphatidylinositol anchor synthesis. Conclusion: the effect of Radix Xuanshen on spleen deficiency model rats is related to improving digestive function, inhibiting glucose metabolism and improving metabolism of blood lipids.
【作者單位】： 黑龍江中醫(yī)藥大學(xué)中醫(yī)藥研究院黑龍江省高等院校中藥藥性理論創(chuàng)新團(tuán)隊(duì)藥物安全性評(píng)價(jià)中心;
【基金】：國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃)項(xiàng)目(2013CB531804)
【分類號(hào)】：R285.5;R-332
【正文快照】： [網(wǎng)絡(luò)出版地址]http://kns.cnki.net/kcms/detail/11.3495.r.20170731.1054.080.html[網(wǎng)絡(luò)出版時(shí)間]2017-07-31 10:54中醫(yī)所論述的“脾”是以消化系統(tǒng)為主的多系統(tǒng)、多器官的功能綜合單位�！捌⒅鬟\(yùn)化”是脾的主要生理功能。脾氣虛則氣化不行,濕邪積滯,氣不化濕,即水濕不化,臨

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