發(fā)布時(shí)間：2018-01-22 08:36

  本文關(guān)鍵詞： 血必凈注射液 缺血-再灌注損傷 下肢 骨骼肌　出處：《山西醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：建立大鼠下肢缺血-再灌注損傷（ischemia-reperfusion injure, IRI）動(dòng)物模型，再灌注即刻給予血必凈注射液，初步探討血必凈對(duì)大鼠下肢缺血-再灌注損傷中的影響，為進(jìn)一步開展臨床上應(yīng)用血必凈防治肢體缺血-再灌注損傷奠定基礎(chǔ)。 方法：健康Wistar大鼠36只，隨機(jī)分為三組，每組12只。（1）假手術(shù)組（sham operationgroup，SOG組），開腹只分離腹主動(dòng)脈，不夾閉腹主動(dòng)脈。（2）缺血再灌注組（I/R組），無創(chuàng)動(dòng)脈夾夾閉腹主動(dòng)脈2h后，再灌注即刻自尾靜脈注射生理鹽水（8ml/kg），松開血管夾恢復(fù)血流灌注4h。（3）血必凈組（XBJ組），夾閉腹主動(dòng)脈2h，再灌注即刻自尾靜脈注射血必凈注射液（8ml/kg），松開血管夾恢復(fù)血流灌注4h。每組動(dòng)物分別在再灌注4h后抽取血液標(biāo)本，分離血清，測(cè)定血清中MPO、LDH、TNOS、iNOS和cNOS的活性以及HE染色后光鏡下觀察骨骼肌、血管壁結(jié)構(gòu)的變化。 結(jié)果：于SOG組比較，I/R組XBJ組血清中MPO、LDH、TNOS、iNOS的活性均增高（P0.05），I/R中血清中cNOS活性降低（P0.05），XBJ組血清中cNOS活性增高（P0.05）。于I/R組比較，XBJ組血清中MPO、LHD、iNOS活性均降低（P0.05），XBJ組血清中TNOS、cNOS活性增高（P0.05）。I/R組再灌注4小時(shí)后骨骼肌細(xì)胞腫脹；細(xì)胞間隙變窄，大量肌細(xì)胞斷裂，細(xì)胞間隙中有大量炎性細(xì)胞浸潤(rùn)。肌纖維水腫，排列不整齊，部分肌纖維有斷裂；血管壁各層排列疏松，細(xì)胞水腫，VECs胞核向管腔突出明顯，VECs與內(nèi)皮下彈性膜結(jié)合松散，部分細(xì)胞脫落至管腔內(nèi)，部分血管內(nèi)皮細(xì)胞缺失，可見炎性細(xì)胞黏附。XBJ組再灌注4小時(shí)骨骼肌細(xì)胞腫脹減輕，細(xì)胞間隙趨于正常，肌細(xì)胞斷裂較少，細(xì)胞間隙中炎性細(xì)胞浸潤(rùn)減少，細(xì)胞核腫脹減輕；肌纖維排列較整齊，肌纖維斷裂較少；VECs排列尚規(guī)則，部分細(xì)胞水腫，與內(nèi)皮下彈性膜結(jié)合較緊密，內(nèi)皮細(xì)胞脫落及炎性細(xì)胞粘附較少 結(jié)論：實(shí)驗(yàn)表明血必凈注射液對(duì)缺血再灌注損傷有一定的保護(hù)作用。其保護(hù)作用主要表現(xiàn)在穩(wěn)定中性粒細(xì)胞，降低iNOS活性，減少iNOS源性NO；增高cNOS活性，使eNOS源性NO增加，，減少對(duì)細(xì)胞的毒性作用，減輕細(xì)胞水腫，降低細(xì)胞膜通透性，恢復(fù)細(xì)胞的正常功能。
[Abstract]:Objective: to establish an animal model of ischemia-reperfusion injury (IRI) in rats and to give Xuebijing injection immediately after reperfusion. To explore the effect of Xuebijing on lower limb ischemia-reperfusion injury in rats, and to lay a foundation for further clinical application of Xuebijing in prevention and treatment of limb ischemia-reperfusion injury. Methods: Thirty-six healthy Wistar rats were randomly divided into three groups (12 rats in each group) sham operationg roupSOG group (sham operation group). The abdominal aorta was isolated from the abdominal aorta without clamping the abdominal aorta in the ischemia reperfusion group. The abdominal aorta was clamped by non-invasive artery for 2 hours. Immediately after reperfusion, 8 ml / kg of normal saline was injected into the caudal vein, and the blood flow perfusion was restored 4 h. 3) in XBJ group, the abdominal aorta was clipped for 2 hours. Immediately after reperfusion, Xuebijing injection was injected into caudal vein at 8 ml / kg 路kg ~ (-1), and blood perfusion was recovered for 4 h by loosening the clip. Blood samples were taken from each group for 4 hours after reperfusion and serum was isolated. The activities of iNOS and cNOS in serum were measured and the changes of skeletal muscle and vascular wall structure were observed under light microscope after HE staining. Results: compared with SOG group, the activity of XBJ in XBJ group was significantly higher than that in XBJ group (P 0.05). The activity of cNOS in the serum of I / R group was lower than that in the group of P0.05 and XBJ, and the activity of cNOS in the serum of the group of P0.05 was significantly higher than that in the group of I / R, compared with that of the group of XBJ in the I / R group. The activity of iNOS decreased in both groups. The activity of TNOS-CNOS increased in the serum of P0.05 + XBJ group and the swelling of skeletal muscle cells was observed in P0.05 + I / R group after 4 hours of reperfusion. The intercellular space became narrow, a large number of muscle cells broke, a large number of inflammatory cells infiltrated in the intercellular space, the muscle fibers were edema, disordered arrangement, and some of the muscle fibers were broken. The layers of vascular wall were loosely arranged, and the VECs nucleus protruded into the lumen. The VECs were loosely bound to the endodermic elastic membrane, some of the cells fell off into the lumen and some of the vascular endothelial cells were missing. It can be seen that the inflammatory cell adhesion. XBJ group reduced the swelling of skeletal muscle cells at 4 hours after reperfusion, the gap tended to be normal, the break of muscle cells was less, the infiltration of inflammatory cells in the intercellular space was decreased, and the swelling of nucleus was alleviated. The arrangement of muscle fibers was neat, and the breakage of muscle fibers was less. The VECs arrangement is regular, some cells are edema, binding to the endodermic elastic membrane is close, endothelial cells fall off and inflammatory cell adhesion is less. Conclusion: Xuebijing injection has a certain protective effect on ischemia-reperfusion injury. The protective effect of Xuebijing injection is mainly in stabilizing neutrophil, decreasing iNOS activity and decreasing iNOS derived no. By increasing the activity of cNOS, the activity of eNOS derived no was increased, the toxic effect of eNOS on cells was reduced, the edema of cells was alleviated, the permeability of cell membrane was decreased, and the normal function of cells was restored.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 代建軍,曹書華,王今達(dá);血必凈對(duì)危重病患者血管內(nèi)皮細(xì)胞的保護(hù)作用研究[J];中國(guó)全科醫(yī)學(xué);2005年18期

2 張?jiān)平?;血必凈注射液對(duì)小腸缺血再灌注大鼠血清細(xì)胞因子的影響[J];山東中醫(yī)藥大學(xué)學(xué)報(bào);2008年06期

3 林福清;鄧小明;朱科明;;“血必凈”注射液對(duì)急性炎癥反應(yīng)綜合征患者中性粒細(xì)胞功能的影響[J];同濟(jì)大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年06期

本文編號(hào)：1454232