本文關(guān)鍵詞： 間充質(zhì)干細(xì)胞 基因表達(dá) 流體切應(yīng)力 信號轉(zhuǎn)導(dǎo)　出處：《醫(yī)用生物力學(xué)》2017年01期 　論文類型：期刊論文

【摘要】：目的探討流體切應(yīng)力對骨髓間充質(zhì)干細(xì)胞(bone marrow mesenchymal stem cells,BMSCs)中Bmi-1基因表達(dá)的影響及可能的信號機制。方法原代體外分離培養(yǎng)大鼠BMSCs,應(yīng)用平行平板流動腔系統(tǒng),給BMSCs施加不同強度(0.5、1.5、3.0 Pa)和不同加載時間(1、2、6、24 h)的層流切應(yīng)力,用實時定量RT-PCR法檢測Bmi-1基因的表達(dá)水平,用免疫印跡法檢測磷酸化Akt和ERK1/2的表達(dá)水平,利用Wortmannin(PI3K特異性抑制劑)、PD98059(ERK1/2 MAPK特異性抑制劑)信號阻斷劑探討信號轉(zhuǎn)導(dǎo)途徑。結(jié)果 BMSCs在1.5 Pa切應(yīng)力作用1 h后Bmi-1基因表達(dá)即明顯增強,24 h達(dá)高峰。不同強度切應(yīng)力都會刺激Bmi-1基因表達(dá),其中3.0 Pa最強。切應(yīng)力能顯著激活磷酸化Akt和ERK1/2表達(dá)。Wortmannin而不是PD98059可以明顯抑制Bmi-1基因的表達(dá)。結(jié)論切應(yīng)力可誘導(dǎo)BMSCs中Bmi-1基因表達(dá),其表達(dá)量與刺激時間和切應(yīng)力的強度密切相關(guān),這種作用可能通過Akt信號調(diào)節(jié)。
[Abstract]:Objective to investigate the effect of fluid shear stress on bone marrow marrow mesenchymal stem cells. Methods Rat BMSCs were isolated and cultured in vitro, and parallel plate flow chamber system was used. The laminar shear stress of BMSCs was applied to BMSCs with different strength of 0.5 ~ (1.5) Pa) and different loading time of 1 ~ 2 ~ 2 ~ 6 ~ 6 ~ (24 h). The expression levels of Bmi-1 gene and phosphorylated Akt and ERK1/2 were detected by real-time quantitative RT-PCR and Western blot. Wortmannin(PI3K specific inhibitors were used. PD98059(ERK1/2 MAPK specific inhibitor. Results the expression of Bmi-1 gene increased significantly after 1 h of BMSCs was exposed to 1.5 Pa shear stress. The peak was reached at 24 h. The Bmi-1 gene expression was stimulated by different shear stress. Of which 3.0. Pa is the strongest. Shear stress can significantly activate the expression of phosphorylated Akt and ERK1/2. Wortmannin, instead of PD98059, can significantly inhibit the expression of Bmi-1 gene. Conclusion Shear stress can significantly inhibit the expression of Bmi-1 gene. It can induce the expression of Bmi-1 gene in BMSCs. The expression level is closely related to the stimulation time and shear stress, which may be regulated by Akt signal.
【作者單位】： 湖北醫(yī)藥學(xué)院附屬隨州醫(yī)院轉(zhuǎn)化醫(yī)學(xué)研究中心;湖北醫(yī)藥學(xué)院基礎(chǔ)醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金項目(31670961) 湖北省科技廳自然基金項目(2015CFB186) 湖北醫(yī)藥學(xué)院大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(2015XS10)
【分類號】：R329.2
【正文快照】： 骨髓間充質(zhì)干細(xì)胞(bone mesenchymal stemcells,BMSCs)是一種來源于中胚層的多能干細(xì)胞,具有多向分化潛能。干細(xì)胞在體內(nèi)生長的局部微環(huán)境(包括細(xì)胞外基質(zhì)、細(xì)胞因子在內(nèi)的基質(zhì)微環(huán)境和復(fù)雜的力學(xué)微環(huán)境共同組成)對其分化走向起重要的調(diào)控作用[1-2]。流體切應(yīng)力(fluid shear s

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