本文關(guān)鍵詞： TIM蛋白 樹突狀細胞 Th細胞 食物過敏 金黃色葡萄球菌腸毒素B 卵清蛋白　出處：《世界華人消化雜志》2011年09期 　論文類型：期刊論文

【摘要】：目的:研究微生物產(chǎn)物對樹突狀細胞(dendriticcell,DC)和TIM4的作用,探討在微生物產(chǎn)物參與的食物過敏(foodallergy,FA)中TIM4對CD4+T淋巴細胞分化的影響.方法:培養(yǎng)Balb/c小鼠骨髓來源的樹突狀細胞(bonemarrow-derived DC,BMDC),培養(yǎng)的DC分為兩組:金黃色葡萄球菌腸毒素B(SEB)刺激組和空白對照組,RT-PCR檢測不同組DC的TIM4 mRNA表達;流式細胞儀檢測DC表面CD11c、MHC-Ⅱ、CD86的表達.在DC和CD4+T淋巴細胞共同培養(yǎng)中將培養(yǎng)的DC分為5組:空白對照組、SEB+卵清蛋白(OVA)組、SEB組、OVA組及TIM4組,同時取過敏模型小鼠脾臟CD4+T淋巴細胞,不同組的DC與CD4+T淋巴細胞共同培養(yǎng),ELISA法測定混合培養(yǎng)細胞上清液IL-4與IFN-γ的表達.結(jié)果:與空白對照組相比,SEB組DCTIM4 mRNA表達明顯增高(0.941±0.018vs0.422±0.083,P0.05),并具有劑量依從性.SEB組DC表面分子MHC-Ⅱ、CD86表達明顯高于空白對照組(MHC-Ⅱ:76.684%±3.1803%vs52.984%±3.6026%;CD86:89.746%±2.113%vs67.558%±0.4341%,均P=0.000).DC和CD4+T淋巴細胞共同培養(yǎng)中,相比空白對照組,SEB+OVA組IL-4表達顯著增高(295.834±20.408vs78.335±13.109,P0.05),而IFN-γ的表達明顯減少(362.109±92.271vs761.897±102.967,P0.05);單獨的SEB組或OVA組IL-4和IFN-γ與空白對照組無明顯差異;TIM4組中IL-4的表達水平較SEB+OVA組明顯降低,而IFN-γ的表達明顯增高(90.511±15.500vs295.834±20.408;807.734±95.436vs362.109±92.271,均P0.05).結(jié)論:微生物產(chǎn)物和食物抗原共同作用導(dǎo)致FA,TIM4參與FA的發(fā)生.消除TIM4的表達可明顯抑制Th2細胞分化,有效糾正Th1/Th2細胞失衡,可阻止過敏性免疫反應(yīng).
[Abstract]:Objective: to study the effects of microbial products on dendritic cell (DC) and TIM4 in dendritic cells. To investigate the involvement of microbial products in food allergy. Methods: dendritic cells derived from bone marrow of Balb/c mice were cultured in vitro. The cultured DC were divided into two groups: staphylococcus aureus enterotoxin group and blank control group. RT-PCR was used to detect the expression of TIM4 mRNA in DC of different groups. Flow cytometry was used to detect the expression of CD11cmHC- 鈪，

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