本文關(guān)鍵詞： 恒河猴 腰椎穿刺術(shù) 腦脊液　出處：《動(dòng)物學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：探索數(shù)字減影血管造影(digital subtraction angiography,DSA)插管技術(shù)在恒河猴(Macaca mulatta)腦脊液動(dòng)態(tài)采集中的應(yīng)用。將28只恒河猴作為實(shí)驗(yàn)動(dòng)物,隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,每組14只,經(jīng)左前肢靜脈推注3%戊巴比妥鈉溶液全麻后,常規(guī)剔除背部脊柱周圍體毛、消毒,實(shí)驗(yàn)組從穿刺開始就在DSA技術(shù)下行腰椎間隙穿刺,對(duì)照組直接行腰椎間隙穿刺,將硬膜外麻醉導(dǎo)管經(jīng)腰椎蛛網(wǎng)膜下腔送至枕大池并埋置泵。對(duì)不同穿刺方法的結(jié)果數(shù)據(jù)進(jìn)行χ2檢驗(yàn);神經(jīng)功能參照改良Tarlov評(píng)分標(biāo)準(zhǔn),切口采用改良的切口愈合等級(jí)評(píng)分標(biāo)準(zhǔn)。實(shí)驗(yàn)組經(jīng)腰椎間隙穿刺蛛網(wǎng)膜下腔全部成功;對(duì)照組8只穿刺成功,6只穿刺失敗;所有動(dòng)物埋置泵均能從泵中采集到無(wú)色透明的腦脊液;術(shù)后恒河猴均于當(dāng)天恢復(fù)正常。在DSA技術(shù)下經(jīng)腰椎間隙穿刺蛛網(wǎng)膜下腔并埋置泵,成功率高,操作簡(jiǎn)便、安全性好,可行性強(qiáng);通過(guò)埋置的泵采集腦脊液,快速省時(shí),可實(shí)現(xiàn)在動(dòng)物清醒時(shí)、全生理狀態(tài)下對(duì)腦脊液進(jìn)行動(dòng)態(tài)多次采集,有助于提高非人靈長(zhǎng)類實(shí)驗(yàn)動(dòng)物的福利,由于采樣導(dǎo)管置于枕大池,采集到的腦脊液樣品質(zhì)量有保證,可用于研究缺血性腦卒中發(fā)生機(jī)制,滿足檢驗(yàn)需要,具有一定的實(shí)用價(jià)值。
[Abstract]:Exploration of digital subtraction angiography (digital subtraction angiography, DSA) intubation technique in Ganges RIver Monkey (Macaca mulatta) application of cerebrospinal fluid dynamic acquisition. 28 Ganges RIver monkey as experimental animal, were randomly divided into experimental group and control group, 14 rats in each group, the left forelimb vein injection of 3% pentobarbital sodium solution after general anesthesia the conventional back around, remove hair, disinfection, the experimental group from the start in the DSA downlink puncture lumbar intervertebral puncture, control group underwent lumbar puncture clearance, epidural anesthesia catheter through lumbar subarachnoid cisterna and sent to the embedded pump. For different puncture methods data was analyzed by 2 test; the nerve function according to the modified Tarlov standard for evaluation, using a modified incision wound healing grade standard for evaluation. Experimental group after lumbar subarachnoid space puncture was successful in all the 8 rats in the control group; 6 successful puncture, puncture failure; All animal were collected from the buried pump pump to clear cerebrospinal fluid; postoperative Ganges RIver monkeys were returned to normal. On the same day under DSA technology after lumbar subarachnoid space puncture and embedding pump, high success rate, simple operation, good safety, strong feasibility; collecting cerebrospinal fluid through the pump, buried the fast time, can realize the animal awake, all under physiological condition of cerebrospinal fluid dynamic repeated acquisition, help to improve the welfare of nonhuman primate experimental animal, because sampling catheters placed in the cisterna, guaranteed the collected samples of cerebrospinal fluid quality, can be used to study the pathogenesis of ischemic stroke, meet the needs of the test. It has certain practical value.

【作者單位】： 四川大學(xué)華西醫(yī)院;成都高新海爾森醫(yī)院;
【基金】：國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)項(xiàng)目(No.2012AA020702)
【分類號(hào)】：R-332;R743.3
【正文快照】： 腦脊液(cerebrospinal fluid,CSF)主要由1材料與方法側(cè)腦室脈絡(luò)叢產(chǎn)生,是充滿在各腦室、蛛網(wǎng)膜下腔和脊髓中央管內(nèi)的無(wú)色透明液體(Levy1.1實(shí)驗(yàn)動(dòng)物2010),其性質(zhì)與血漿和淋巴液相似,略帶黏性,恒河猴28只,雄性,體重4~7 kg,年齡對(duì)中樞神經(jīng)系統(tǒng)起著支持、營(yíng)養(yǎng)的作用3~6歲,購(gòu)自四川

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本文編號(hào)：1442548