本文關(guān)鍵詞：大鼠脂肪源性干細(xì)胞來(lái)源的施萬(wàn)樣細(xì)胞的增殖能力　出處：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年01期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 脂肪源性干細(xì)胞 施萬(wàn)樣細(xì)胞 細(xì)胞增殖 有絲分裂

【摘要】：目的:通過(guò)檢測(cè)大鼠脂肪源性干細(xì)胞(ADSCs)來(lái)源的施萬(wàn)樣細(xì)胞的增殖能力,探討其在神經(jīng)修復(fù)再生領(lǐng)域的應(yīng)用及其意義。方法:取大鼠附睪旁脂肪組織進(jìn)行ADSCs的分離與培養(yǎng);將第4代ADSCs在β-巰基乙醇(β-ME)、反式維甲酸(ATRA)、血小板衍生因子AA(PDGF-AA)、堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)、腺苷酸環(huán)化酶激活劑(Forskolin)和Heregulin-β的聯(lián)合作用下誘導(dǎo)分化為施萬(wàn)樣細(xì)胞。采用免疫熒光法、Western blotting法和Real-time PCR法檢測(cè)施萬(wàn)細(xì)胞(SCs)標(biāo)記物S-100β、P75和膠質(zhì)纖維酸性蛋白(GFAP)的表達(dá)水平;采用MTT法檢測(cè)施萬(wàn)樣細(xì)胞的增殖能力,觀察其有絲分裂增殖現(xiàn)象。結(jié)果:誘導(dǎo)分化后的施萬(wàn)樣細(xì)胞呈梭形、柵欄樣排列。S-100β、P75和GFAP蛋白免疫熒光為Cy3/FITC標(biāo)記,陽(yáng)性產(chǎn)物位于細(xì)胞漿及其突起。Western blotting和Real-time PCR法檢測(cè),施萬(wàn)樣細(xì)胞中S-100β、P75和GFAP蛋白和mRNA表達(dá)水平明顯高于ADSCs(P0.01)。MTT法檢測(cè),施萬(wàn)樣細(xì)胞有較強(qiáng)的有絲分裂增殖能力。結(jié)論:ADSCs能夠成功被誘導(dǎo)分化為施萬(wàn)樣細(xì)胞,并表達(dá)SCs標(biāo)記物;施萬(wàn)樣細(xì)胞有較強(qiáng)的有絲分裂增殖能力,可作為神經(jīng)組織工程理想的種子細(xì)胞。
[Abstract]:Objective: to investigate the proliferative ability of Schwann like cells derived from adipose derived stem cells (ADSCs) in rats. Methods: ADSCs was isolated and cultured from the adipose tissue of rat epididymis. The fourth generation of ADSCs was detected in 尾 -mercaptoethanol (尾 -MEA), ATRAA (trans-retinoic acid), PDGF-AAA (platelet-derived factor). Basic fibroblast growth factor (bFGF). Adenylate cyclase activator Forskolin) and Heregulin- 尾 were used to induce differentiation into Schwan-like cells. S-100 尾 was detected by Western blotting and Real-time PCR. The expression level of P75 and GFAP; The proliferative ability and mitotic proliferation of Schwan-like cells were detected by MTT method. Results: Schwan-like cells were fusiform, palisade like arrangement. S-100 尾 after induced differentiation. P75 and GFAP proteins were labeled with Cy3/FITC. The positive products were detected by Western blotting and Real-time PCR in cytoplasm and its processes. S-100 尾 was detected in Schwan-like cells. The expression levels of P75 and GFAP protein and mRNA were significantly higher than those detected by ADSCs(P0.01).MTT. Conclusion: Schwan-like cells can be successfully induced to differentiate into Schwan-like cells and express SCs markers. Schwann like cells have strong mitotic and proliferative ability and can be used as ideal seed cells for nerve tissue engineering.
【作者單位】： 承德醫(yī)學(xué)院人體解剖學(xué)教研室;河北省承德市婦幼保健醫(yī)院兒科;承德醫(yī)學(xué)院預(yù)防醫(yī)學(xué)教研室;承德醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)研究所;
【基金】：河北省教育廳科研基金資助課題(QN2014138)
【分類(lèi)號(hào)】：R329.2
【正文快照】： 施萬(wàn)細(xì)胞(Schwann cells,SCs)是周?chē)窠?jīng)的膠質(zhì)細(xì)胞,具有神經(jīng)營(yíng)養(yǎng)、趨化和使再生神經(jīng)纖維成熟的功能。周?chē)窠?jīng)損傷后,SCs不但能夠增生、遷移,形成Büngner帶,作為橋梁引導(dǎo)再生神經(jīng)纖維向遠(yuǎn)端生長(zhǎng)并到達(dá)靶器官,還能分泌多種神經(jīng)營(yíng)養(yǎng)因子和神經(jīng)生長(zhǎng)因子,誘導(dǎo)并促進(jìn)軸突生長(zhǎng),為

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