本文關鍵詞：植物乳桿菌微膠囊制備技術及其消化道耐受性研究　出處：《南昌大學》2012年碩士論文　論文類型：學位論文

  更多相關文章： 益生菌 微膠囊化 真空干燥 植物乳桿菌 凍干保護劑 擠壓法 兩步真空干燥法

【摘要】：作為一類與宿主互利共生、有利于宿主健康的生理細菌,益生菌能夠通過改善腸道微生態(tài)平衡而促進人體健康。 益生菌制劑就是利用益生菌能夠調(diào)節(jié)腸道菌群生態(tài)平衡,對宿主起有益作用的特點而制成的活性微生態(tài)制劑,乳桿菌是常用的益生菌菌種。但是消化道中低pH值的胃酸和膽汁會給活性益生菌造成很大的殺滅,極大地影響了益生菌在腸道中定植并發(fā)揮生理功能。 本研究采用微膠囊化技術與真空干燥技術相結合的方法,以海藻酸鈉和脫脂乳作為混合壁材,將植物乳桿菌和凍干保護劑一起作為芯材,對益生菌進行包埋,并進行真空干燥處理以減少膠囊中的水分,延長益生菌制劑的貯存時間,使益生菌能夠順利通過胃液到達腸道中發(fā)揮益生作用。 本試驗研究了擠壓法制備腸溶性植物乳桿菌微膠囊的工藝,通過優(yōu)化設計確定了最佳制備條件,同時優(yōu)化了冷凍干燥過程中復合保護劑的成分。此外,還采用改良的兩步真空干燥法替代了傳統(tǒng)的真空冷凍干燥法,提高了凍干膠囊中的菌體存活率。并在此基礎上通過模擬胃腸液處理、貯存穩(wěn)定性處理等應用性研究,對膠囊產(chǎn)品進行了性能評估。研究內(nèi)容和結果如下： 1、利用海藻酸鈉形成的膠囊具有腸溶而胃不溶的特點以及乳清蛋白在低pH值條件且有胃蛋白酶存在的情況下依然能夠保持結構完整的特性,將海藻酸鈉和脫脂乳作為植物乳桿菌微膠囊化的混合壁材。通過響應面優(yōu)化設計,確定擠壓法制微膠囊的最佳工藝條件為：海藻酸鈉質(zhì)量分數(shù)2.68%,氯化鈣濃度0.20mol/L,脫脂乳質(zhì)量分數(shù)4.17%。按此工藝制備的微膠囊在胃液中處理3h后,微膠囊中的活菌數(shù)可達8.79×109CFU/g。 2、在單因素試驗的基礎上篩選得到4種在冷凍干燥過程中對植物乳桿菌保護效果較好的凍干保護劑,通過正交試驗得到最佳復合凍干保護劑配方為：海藻糖8%、乳糖8%、維生素C0.2%、甘油3%。用此配方凍干微膠囊,可使菌體存活率達到87.2%。 3、利用改良的兩步真空干燥法即真空恒溫干燥和真空冷凍干燥相結合的方法,對微膠囊進行干燥處理。不僅可以縮短真空冷凍干燥的時間,而且可以使凍干膠囊中的活菌細胞存活率提高至90.3%。 4、按照優(yōu)化工藝制備獲得的植物乳桿菌微膠囊在人工胃液中處理3h后,活菌菌體存活率可以達到80%左右,說明經(jīng)過微膠囊化處理,益生菌菌體對胃酸環(huán)境的抵抗力得到增強。 5、按照優(yōu)化工藝制備獲得的植物乳桿菌膠囊在人工腸液中處理45min后,溶液的透光率降至最低值,且看不到固體顆粒,說明微膠囊已基本崩解完全,達到中華人民共和國藥典2005年版標準的要求。由此可以判斷,按照本實驗優(yōu)化工藝制備的植物乳桿菌微膠囊具備良好的腸溶性。 6、凍干膠囊在-20℃條件下貯存60d后,微膠囊中菌體存活率接近70%。在2℃條件下貯存60d后,仍然有接近60%的存活率。說明采用本實驗優(yōu)化工藝獲得的微膠囊化方法可以有效提高植物乳桿菌在低溫下的貯存穩(wěn)定性。
[Abstract]:As a kind of physiological bacteria that is beneficial to the host's health, probiotics can promote the health of the human body by improving the intestinal microecological balance.
Is the use of probiotics probiotics can regulate the ecological balance of intestinal flora, made the activity of probiotics on host characteristics beneficial role of Lactobacillus probiotics is commonly used. But the digestive tract and bile acid of low pH value can cause much to kill activity of probiotics, greatly affected the probiotics in intestinal tract and play a physiological function.
This research adopts the method of microencapsulation technology and vacuum drying technology combined with sodium alginate, and skim milk as a mixture of wall material, Lactobacillus plantarum and protectants as the core material, the embedding of probiotics, and vacuum drying treatment to reduce the moisture in the capsule, prolong the storage time of probiotics so, probiotics can arrive smoothly through the gastric juice probiotic in the gut.
The experimental study on the preparation of the enteric microcapsule of Lactobacillus plantarum by extrusion, by optimizing the design to determine the optimum preparation conditions, and optimize the composition of composite protective agent during freeze drying process. In addition, the two step modified vacuum drying method instead of the traditional vacuum freeze drying method, improved freeze drying capsule in the survival rate of bacteria. And on the basis of simulated gastrointestinal treatment, storage stability and application research, to evaluate the performance of capsule products. The research contents and results are as follows:
1, the use of sodium alginate with enteric capsule formation and characteristics of gastric insoluble and whey protein in low pH value and is still able to maintain structural integrity of the characteristics of the presence of pepsin, sodium alginate and skim milk mixture as wall material of microencapsulation of Lactobacillus plantarum. Through the optimization design of the response surface, to determine the optimum process the conditions for extrusion of microcapsules: sodium alginate concentration 2.68%, calcium chloride concentration 0.20mol/L, microcapsule skim milk mass fraction of 4.17%. was prepared by the procedure of 3H in gastric juice, bacteria microcapsule in number can reach 8.79 * 109CFU/g.
2, on the basis of single factor test were 4 in the freeze-drying process of Lactobacillus plantarum better protective effect of cryoprotectant, the optimal compound cryoprotector formula by orthogonal test: 8% trehalose, lactose 8%, vitamin C0.2%, glycerol 3%. with the prescription of lyophilized microcapsules the cell survival rate reached 87.2%.
3, we use the improved two step vacuum drying method, namely vacuum constant temperature drying and vacuum freeze-drying, to dry the microcapsule. It can not only shorten the time of vacuum freeze-drying, but also increase the viability of viable cells in lyophilized capsules to 90.3%..
4, according to the optimized process, the survival rate of viable Lactobacillus microcapsules in the artificial gastric juice after treatment with 3H is about 80%, indicating that after microencapsulation, the resistance of probiotics to gastric acid environment is enhanced.
In 5, 45min in the artificial intestinal juice with Lactobacillus capsule preparation process optimized solution is obtained, the transmittance decreased to the minimum value, and not to the solid particles, indicating that the micro capsule has been basically complete disintegration, People's Republic of China reached the 2005 edition of the Pharmacopoeia standards. It can be judged, in accordance with the optimum process of Lactobacillus plantarum preparation of microcapsules with enteric coated well.
6, the freeze-dried 60d capsule storage at -20 deg.c, microcapsule cell survival rate close to 70%. storage 60d under the condition of 2 DEG C, there are still nearly 60% survival rate. So the optimized microencapsulation process obtained can effectively improve the storage stability of Lactobacillus plantarum in low temperature.

【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R371

【參考文獻】

相關期刊論文 前10條

1 陳麟鳳;劉景漢;;常用細胞凍干保護劑的特性、作用機制及應用進展[J];中國輸血雜志;2006年06期

2 李義海,謝宏;活菌制劑在反芻家畜飼養(yǎng)中的應用[J];飼料工業(yè);1998年01期

3 秦華明,宗敏華,梁世中;糖在蛋白質(zhì)藥物冷凍干燥過程中保護作用的分子機制[J];廣東藥學院學報;2001年04期

4 徐凱進,李蘭娟;腸道正常菌群與腸道免疫[J];國外醫(yī)學.流行病學傳染病學分冊;2005年03期

5 張董燕;季海峰;徐煒玲;;益生菌對動物腸道微生物生態(tài)學影響的研究進展[J];中國畜牧獸醫(yī);2007年03期

6 杜鵬,楊玉琢,霍貴成;雙歧桿菌凍干制劑貯藏存活能力的研究[J];現(xiàn)代食品科技;2005年04期

7 呂利軍;季海峰;單達聰;張董燕;;乳酸菌類微生物制劑微膠囊化的研究進展[J];黑龍江畜牧獸醫(yī);2008年08期

8 陳思，

本文編號：1438143