本文關(guān)鍵詞：補(bǔ)腎溫陽(yáng)祛風(fēng)散寒法對(duì)腎陽(yáng)虛型圍絕經(jīng)期模型大鼠ER、VEGF、HSP70作用的研究　出處：《遼寧中醫(yī)雜志》2016年05期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 補(bǔ)腎溫陽(yáng)祛風(fēng)散寒法 雌激素受體 血管內(nèi)皮生長(zhǎng)因子 熱休克蛋白

【摘要】：目的:探討補(bǔ)腎溫陽(yáng)祛風(fēng)散寒法對(duì)腎陽(yáng)虛型圍絕經(jīng)期模型大鼠ER、VEGF、HSP70的影響及作用機(jī)制。方法:選用自然老化的圍絕經(jīng)期雌性SD大鼠,肌注氫化可的松構(gòu)建腎陽(yáng)虛型圍絕經(jīng)期大鼠模型,將模型大鼠隨機(jī)分為更年湯組、教材方組、替勃龍組、生理鹽水組;另選青年組、老年組SD雌性大鼠作為對(duì)照組。各組給予相應(yīng)藥物治療15 d,于第16天處死大鼠,分離摘取雙側(cè)子宮,稱(chēng)重了解子宮濕重及子宮指數(shù),采用病理切片觀察大鼠子宮形態(tài)學(xué)變化,免疫組化及原位雜交檢測(cè)方法觀察大鼠子宮雌激素受體(ER)、血管內(nèi)皮生長(zhǎng)因子(VEGF)、熱休克蛋白70(HSP70)及信使mRNA(ERmRNA、VEGFmRNA、HSP70mRNA)的陽(yáng)性表達(dá)情況。結(jié)果:1更年湯組、教材方組、替勃龍組能明顯增加子宮濕重、子宮指數(shù),有統(tǒng)計(jì)學(xué)意義(P0.05),更年湯組與替勃龍組、教材方組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);2更年湯組、教材方組、替勃龍組能抑制子宮萎縮,有顯著性差異(P0.01),更年湯組與替勃龍組比較無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);3更年湯組、教材方組、替勃龍組能增加ER、ERmRNA、VEGF、VEGFmRNA陽(yáng)性表達(dá),有統(tǒng)計(jì)學(xué)意義(P0.05),但教材方組的上調(diào)低于更年湯組及替勃龍組(P0.05),替勃龍組與更年湯組之間比較無(wú)顯著差異(P0.05);4更年湯組、替勃龍組能增加HSP70及HSP70mRNA陽(yáng)性表達(dá),有統(tǒng)計(jì)學(xué)意義(P0.05),更年湯組與替勃龍組比較無(wú)顯著性差異(P0.05)。結(jié)論:自擬更年湯可能通過(guò)上調(diào)ER、VEGF、HSP70蛋白及基因在子宮中的陽(yáng)性表達(dá)而發(fā)揮雌激素樣作用,從而達(dá)到改善病理狀態(tài)下的子宮的形態(tài)結(jié)構(gòu)及功能的目的。
[Abstract]:Objective: To investigate the kidney yang Qufeng method on kidney yang deficiency perimenopausal rat model of ER, VEGF, HSP70 and its mechanism. Methods: the natural aging of the perimenopausal female SD rats, injection of hydrocortisone in construction of kidney yang deficiency model peri climacteric rat model, the rats were randomly divided into Gengnian Decoction group, teaching group, tibolone group, saline group; another youth group and elderly group SD female rats as the control group. Each group was given corresponding drug treatment of 15 d on the sixteenth day, the rats were sacrificed and the separation from bilateral uterine, weighing about uterine wet weight and index of uterus, the pathological observation the morphological changes of uterus in rats, immunohistochemistry and in situ hybridization method to observe the rat uterine estrogen receptor (ER), vascular endothelial growth factor (VEGF), heat shock protein 70 (HSP70) and mRNA (ERmRNA, VEGFmRNA, Messenger HSP70mRNA) positive expression. Results :1鏇村勾姹ょ粍,鏁欐潗鏂圭粍,鏇垮媰榫欑粍鑳芥槑鏄懼鍔犲瓙瀹箍閲，

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