本文關(guān)鍵詞：產(chǎn)氣腸桿菌新德里金屬β-內(nèi)酰胺酶質(zhì)粒分子特征分析　出處：《中華醫(yī)院感染學(xué)雜志》2017年04期 　論文類型：期刊論文

  更多相關(guān)文章： 產(chǎn)氣腸桿菌 NDM- β-內(nèi)酰胺酶

【摘要】：目的分析產(chǎn)氣腸桿菌攜帶blaNDM-1質(zhì)粒的轉(zhuǎn)移性、質(zhì)粒復(fù)制子分型及blaNDM-1的基因環(huán)境。方法產(chǎn)氣腸桿菌HN-NDM0711為研究對(duì)象,接合實(shí)驗(yàn)鑒定質(zhì)粒轉(zhuǎn)移性,PCR方法對(duì)質(zhì)粒復(fù)制子分型,染色體步移技術(shù)對(duì)blaNDM-1上下游測(cè)序,基因組序列比對(duì)使用BLASTN和BLASTP,注釋使用Vector NTI 11.5.1。結(jié)果產(chǎn)氣腸桿菌HN-NDM0711接合實(shí)驗(yàn)陽(yáng)性,質(zhì)粒復(fù)制子為IncA/C型;blaNDM-1位于不常見的插入序列ISAba14和IS91之間;在blaNDM-1上游出現(xiàn)一個(gè)Tn3轉(zhuǎn)座子和I型整合子,整合子上含有一個(gè)由龐大鑲嵌序列構(gòu)成的罕見耐藥基因盒。結(jié)論 IncA/C型質(zhì)粒pHN-NDM0711攜帶blaNDM-1及耐藥基因盒來(lái)源于不同抗菌藥物選擇壓力環(huán)境下的基因重組。只有嚴(yán)格控制臨床、工業(yè)和農(nóng)業(yè)抗菌藥物的合理使用才能從源頭上減少此類細(xì)菌的產(chǎn)生。
[Abstract]:Objective to analyze the transferability of Enterobacter aerogenes carrying blaNDM-1 plasmid. Methods Enterobacter aerogenes HN-NDM0711 was used as the research object. PCR method was used to type plasmid replicas, chromosome step technique was used to sequence blaNDM-1 upstream and downstream, and BLASTN and BLASTP were used for genome sequence alignment. Vector NTI 11.5.1.Results the HN-NDM0711 conjugation test of Enterobacter aerogenes was positive, and the plasmid replicon was IncA/C type. BlaNDM-1 is located between the unusual insert sequence ISAba14 and IS91; A Tn3 transposon and type I integron appeared in the upstream of blaNDM-1. The integron contains a rare drug resistant gene box composed of large mosaic sequences. Conclusion. IncA/C plasmid pHN-NDM0711 carries blaNDM-1 and drug resistance gene cassette from gene recombination under different antimicrobial selection pressure. The rational use of industrial and agricultural antimicrobial agents can reduce the production of such bacteria at the source.
【作者單位】： 鄭州大學(xué)附屬腫瘤醫(yī)院檢驗(yàn)科;
【分類號(hào)】：R378.2
【正文快照】： 新德里金屬β-內(nèi)酰胺酶(NDM-1)的出現(xiàn)引起了世界對(duì)獲得性金屬類β-內(nèi)酰胺酶的普遍關(guān)注[1-2]。研究表明,NDM-1多發(fā)現(xiàn)于大腸埃希菌和肺炎克雷伯菌等細(xì)菌中[2-3],常存在于IncA/C、IncF、IncR等不同類型的質(zhì)粒上[2-3],blaNDM-1周圍存在不同的基因環(huán)境[3]。在以往的實(shí)驗(yàn)中,發(fā)現(xiàn)了三

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