本文關(guān)鍵詞：骨橋蛋白及NF-κB的表達(dá)與大鼠腹主動脈瘤發(fā)生的相關(guān)性　出處：《華中科技大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 腹主動脈瘤 大鼠 骨橋蛋白 NF-κB MMP-2

【摘要】：目的構(gòu)建大鼠腹主動脈瘤模型并觀察大鼠腹主動脈瘤中骨橋蛋白（OPN）、核因子-κB（NF-κB）及基質(zhì)金屬蛋白酶-2（MMP-2）的表達(dá)情況，從而探討大鼠腹主動脈瘤發(fā)生的分子機(jī)制。 方法酶灌注法構(gòu)建大鼠腹主動脈瘤模型,首次手術(shù)中測量大鼠腹主動脈直徑，，術(shù)后2周再次手術(shù)，測量腹主動脈直徑、取材；HE染色法檢測腹主動脈的結(jié)構(gòu)改變及炎性細(xì)胞浸潤情況；特殊染色法檢測腹主動脈中層彈力蛋白變化情況；免疫組化技術(shù)、WesternBlot法檢測各組腹主動脈組織中OPN、NF-κB及基質(zhì)金屬蛋白酶-2（MMP-2）的表達(dá)量。 結(jié)果成功構(gòu)建大鼠腹主動脈瘤模型；腹主動脈瘤組術(shù)后直徑擴(kuò)張率（220.3%）明顯高于對照組（112.3%）；HE染色結(jié)果顯示腹主動脈瘤組動脈壁中各層結(jié)構(gòu)紊亂，炎性細(xì)胞浸潤程度顯著增高；維多利亞特殊染色顯示腹主動脈瘤組動脈壁有不同程度的彈力纖維消失，萎縮膠原纖維量大量增加；免疫組化結(jié)果顯示OPN在腹主動脈瘤組、對照組及正常組動脈壁中的表達(dá)分別為7.5×1012±2.92×1011、3.07×10~5±2.58×10~5、0（F=6.59，P=0.004）；NF-κB在腹主動脈瘤組、對照組及正常組動脈壁中表達(dá)量分別為2.36×10~(10)±2.80×10~(10)、0、0（F=6.96，P=0.004）；MMP-2在腹主動脈瘤組、對照組及正常組動脈壁中的表達(dá)量分別是4.52×10~(10)±1.73×10~(10)、0、0（F=6.83，P=0.003）；WesternBlot結(jié)果顯示OPN在腹主動脈瘤組、對照組及正常組動脈壁中的表達(dá)分別為2.77×10~5±2.82×10~5、4.52×10~3±1.36×10~3、2.11×10~3±1.99×10~3（F=9.49，P=0.001）；NF-κB在腹主動脈瘤組、對照組及正常組動脈壁中表達(dá)量分別為6.014×10~4±6.27×10~4、1.34×10~3±1.89×10~3、4.35×10~2±7.01×10~2（F=8.91，P=0.001）；MMP-2在腹主動脈瘤組、對照組及正常組動脈壁中的表達(dá)量分別是9.25×10~4±6.87×10~4、8.00×10~2±7.89×10~2、2.70×10~2±3.89×10~2（F=17.91，P㩳0.001），OPN、NF-κB及基質(zhì)金屬蛋白酶-2（MMP-2）呈正相關(guān)。 結(jié)論成功構(gòu)建大鼠腹主動脈瘤模型，骨橋蛋白、NF-κB及MMP-2與大鼠腹主動脈瘤形成存在相關(guān)性，骨橋蛋白可能通過NF-κB途徑上調(diào)MMP的表達(dá),過度表達(dá)的MMP加速了動脈壁中細(xì)胞外基質(zhì)的降解而在大鼠腹主動脈瘤的發(fā)生發(fā)展中起到重要作用。
[Abstract]:Objective to establish a rat model of abdominal aortic aneurysm and to observe the expression of osteopontin (OPN), nuclear factor- 魏 B (NF- 魏 B) and matrix metalloproteinase-2 (MMP-2) in abdominal aortic aneurysm. To explore the molecular mechanism of abdominal aortic aneurysm in rats. Methods A rat model of abdominal aortic aneurysm was established by enzyme perfusion. The diameter of abdominal aorta was measured in the first operation. The changes of abdominal aorta structure and inflammatory cell infiltration were detected by HE staining. The changes of elastin in abdominal aorta were detected by special staining method. The expression of OPN- 魏 B and matrix metalloproteinase-2 (MMP-2) in abdominal aorta was detected by Western blot. Results A rat model of abdominal aortic aneurysm was successfully established. The diameter expansion rate of abdominal aortic aneurysm group (220.3%) was significantly higher than that of control group (112.3%). The results of HE staining showed that in the abdominal aortic aneurysm group, the structure of each layer was disordered, and the degree of inflammatory cell infiltration was significantly increased. Victorian special staining showed that elastic fibers disappeared and the amount of atrophy collagen fibers increased significantly in abdominal aortic aneurysm group. Immunohistochemical results showed that the expression of OPN was 7.5 脳 1012 鹵2.92 脳 1011 in abdominal aortic aneurysm group, control group and normal group, respectively. 3.07 脳 10 ~ (5) 鹵2.58 脳 10 ~ (5) F ~ (6. 59) P ~ (2 +) ~ (0.004); The expression of NF- 魏 B in abdominal aortic aneurysm group, control group and normal group were 2.36 脳 10 ~ (10)) 鹵2.80 脳 10 ~ (10) ~ (10) ~ (10) ~ (10) F ~ (6) 6. 96 respectively. P0. 004; The expression of MMP-2 in abdominal aortic aneurysm group, control group and normal group were 4.52 脳 10 ~ (10) 鹵1.73 脳 10 ~ (10) ~ (10) ~ (10) ~ (10) F ~ (6) 3 respectively. P0. 003; WesternBlot results showed that the expression of OPN was 2.77 脳 10 ~ 5 鹵2.82 脳 10 ~ 5 in abdominal aortic aneurysm group, control group and normal group, respectively. 4.52 脳 10 ~ (3) 鹵1.36 脳 10 ~ (3) C 2.11 脳 10 ~ (3) 鹵1.99 脳 10 ~ (3) F ~ (9.49) C ~ (-1); The expression of NF- 魏 B was 6.014 脳 10 ~ 4 鹵6.27 脳 10 ~ 4 in abdominal aortic aneurysm group, control group and normal group, respectively. 1.34 脳 10 ~ (3) 鹵1.89 脳 10 ~ (3) C 4.35 脳 10 ~ (-2) 鹵7.01 脳 10 ~ (2) F ~ (8.91) F ~ (2 +) ~ (0.001); The expression of MMP-2 in abdominal aortic aneurysm group, control group and normal group were 9.25 脳 10 ~ (4) 鹵6.87 脳 10 ~ (4). 8.00 脳 10 ~ (2) 鹵7.89 脳 10 ~ (2) C ~ (2.70 脳 10 ~ (2)) 鹵3.89 脳 10 ~ (2) F ~ (17. 91)? There was a positive correlation between NF- 魏 B and matrix metalloproteinase-2 (MMP-2). Conclusion A rat model of abdominal aortic aneurysm was successfully established. Osteopontin NF- 魏 B and MMP-2 were correlated with the formation of abdominal aortic aneurysm in rats. Osteopontin may up-regulate the expression of MMP through NF- 魏 B pathway. Overexpression of MMP accelerates the degradation of extracellular matrix in arterial wall and plays an important role in the development of abdominal aortic aneurysm in rats.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R-332;R735.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 江少杰,景在平,趙新;吲哚美辛抑制大鼠腹主動脈瘤基質(zhì)金屬蛋白酶9表達(dá)的研究[J];第二軍醫(yī)大學(xué)學(xué)報;2005年06期

2 馮翔,景在平;腹主動脈瘤組織中基質(zhì)金屬蛋白酶的蛋白質(zhì)水平研究[J];解放軍醫(yī)學(xué)雜志;2001年09期

3 許文平;邱健;詹純列;何柏身;馬國亮;謝志泉;;Wistar大鼠腹主動脈瘤模型的建立及改進(jìn)[J];醫(yī)學(xué)研究生學(xué)報;2009年04期

4 聶斌;周洪蓮;糜濤;肖幸;;骨橋蛋白表達(dá)與人主動脈瘤發(fā)生的相關(guān)性[J];臨床心血管病雜志;2009年08期

5 聶斌;周洪蓮;糜濤;肖幸;;核轉(zhuǎn)錄因子-κB表達(dá)與人主動脈瘤發(fā)生的相關(guān)性[J];臨床心血管病雜志;2010年08期

6 劉萍,陳匯,程焰,曾繁典,唐朝樞;大鼠動脈內(nèi)膜損傷后血管中的骨橋蛋白和基質(zhì)Gla蛋白mRNA的表達(dá)變化[J];生理學(xué)報;1999年05期

7 糜濤;聶斌;張存泰;周洪蓮;;The Elevated Expression of Osteopontin and NF-κB in Human Aortic Aneurysms and Its Implication[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2011年05期

8 孫愛軍,高平進(jìn),朱鼎良,姬開達(dá),劉建軍;骨橋蛋白在血管外膜中的表達(dá)及其對外膜成纖維細(xì)胞遷移的影響[J];中華心血管病雜志;2003年05期

9 曾愛輝;譚茗月;;骨橋蛋白與慢性心力衰竭的關(guān)系研究進(jìn)展[J];中國現(xiàn)代藥物應(yīng)用;2009年18期

本文編號：1411436