本文關鍵詞：使用斑馬魚模型比較山豆根不同有效部位的毒性（英文）　出處：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年09期 　論文類型：期刊論文

  更多相關文章： 山豆根 有效部位 肝臟毒性 心血管毒性 斑馬魚

【摘要】：目的:利用斑馬魚模型評價和比較山豆根不同提取方法提取的有效部位的體內(nèi)毒性。創(chuàng)新點:首次在斑馬魚模型中證明山豆根提取方法不同,有效部位的毒性有明顯差異。研究結果有助于指導山豆根的新藥開發(fā)與臨床應用。方法:用五種不同的溶劑(去堿水、乙醇、正丁基乙醇、二氯甲烷和乙醚)提取山豆根,然后通過高效液相色譜法(HPLC)檢測有效部位,將AB品系斑馬魚分為對照組(養(yǎng)魚水處理)和實驗組(山豆根提取物)。實驗組根據(jù)采用的提取溶劑不同,分為以下六組:去堿水提取組、乙醇沉提取組、正丁基乙醇提取組、二氯甲烷提取組和乙醚提取組以及山豆根總組分組(對照),觀察各種山豆根提取物對斑馬魚的急性毒性與毒性靶器官。結論:山豆根乙醚提取組誘發(fā)斑馬魚心血管毒性(圖1);山豆根去堿水提取組、乙醇沉提取組以及山豆根總組分組誘發(fā)斑馬魚肝臟毒性(圖3和圖4);而山豆根正丁基乙醇提取組和二氯甲烷提取組誘發(fā)斑馬魚心血管毒性(圖1和圖2)和肝臟毒性(圖3和圖4)。
[Abstract]:Objective: to evaluate and compare in vivo toxicity of the effective parts extracted by different extraction methods of soybean root by using zebrafish model. Innovation: it is the first time to prove that the extraction methods are different in zebrafish model. There were significant differences in toxicity of active parts. The results were helpful to guide the development of new drugs and clinical application. Methods: five different solvents (desalination, ethanol and Ding Ji ethanol) were used. Dichloromethane and ether were used to extract the root and then the effective fraction was determined by HPLC. AB strain zebrafish were divided into control group (fish culture water treatment) and experimental group (bean root extract). According to the extraction solvent, the experimental group was divided into the following six groups: desalinated water extraction group, ethanol precipitation extraction group. Ding Ji ethanol extraction group, dichloromethane extraction group, ether extraction group and total group (control). To observe the acute toxicity and toxic target organ of various extracts of soybean root to zebrafish. Conclusion: the cardiovascular toxicity of zebrafish was induced in the extract group (fig. 1). The hepatotoxicity of zebrafish was induced in three groups (Fig. 3 and Fig. 4). The cardiovascular toxicity (Figs. 1 and 2) and liver toxicity (Figs. 3 and 4) of zebrafish were induced by ethanol extraction and dichloromethane extraction.
【作者單位】： Center
【基金】：Project supported by the National Science and Technology Major Project of China(Nos.2012ZX09505001-002 and 2015ZX09501004-002-002) the Zhejiang Provincial Science and Technology Planning Project of China(No.2014C03009)
【分類號】：R285.1;R-332
【正文快照】： http://dx.doi.org/10.1631/jzus.B1600158 CLC number:R285.51 IntroductionThe dried roots and rhizomes of Sophoraetonkinensis Gagnep,commonly known as radixSophorae tonkinensis(RST)or“Shandougen”in Chi-nese,have been used for hundreds of years as a Tra-di

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