本文關(guān)鍵詞：細(xì)粒棘球絳蟲(chóng)轉(zhuǎn)酮醇酶生物信息學(xué)分析　出處：《中國(guó)病原生物學(xué)雜志》2017年03期 　論文類(lèi)型：期刊論文

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【摘要】：目的利用生物信息學(xué)技術(shù)對(duì)EgTK基因編碼蛋白的結(jié)構(gòu)和功能進(jìn)行預(yù)測(cè)和分析。方法利用ExPASy系統(tǒng)的ProtParam、ProtScale、SignalP、SOMPA程序與SubLoc v1.0、DNAStar、IEDB、SYPEITHI、Phyre2等生物信息學(xué)軟件分析EgTK的理化性質(zhì)、抗原表位、跨膜區(qū)、二、三級(jí)結(jié)構(gòu)等。結(jié)果 EgTK基因由7個(gè)外顯子,6個(gè)內(nèi)含子構(gòu)成,CDS長(zhǎng)度為1 878bp,編碼625個(gè)氨基酸,分子式為C_(3015)H_(4791)N_(829)O_(900)S_(22),分子質(zhì)量為67.76ku;為跨膜蛋白,位于細(xì)胞質(zhì),二級(jí)結(jié)構(gòu)主要以α螺旋、無(wú)規(guī)則卷曲為主。EgTK有16個(gè)潛在的B細(xì)胞線(xiàn)性表位,11個(gè)CTL細(xì)胞表位,13個(gè)Th細(xì)胞表位;EgTK三級(jí)結(jié)構(gòu)與人類(lèi)轉(zhuǎn)酮醇酶相似性為97%;經(jīng)多重比對(duì),除去兩端多余序列,共有473個(gè)氨基酸殘基參與比對(duì),發(fā)現(xiàn)195個(gè)變異位點(diǎn)、123簡(jiǎn)約性信息位點(diǎn)、72單態(tài)突變位點(diǎn)。結(jié)論生物信息學(xué)技術(shù)分析EgTK蛋白存在多個(gè)B、T細(xì)胞表位,具有良好的免疫原性,可為該蛋白的基因克隆、表達(dá)等提供理論依據(jù)。
[Abstract]:Objective to predict and analyze the structure and function of EgTK gene encoding protein by bioinformatics. Methods ProtParamia ProtScale of ExPASy system was used. The SignalPu SOMPA program and SubLoc v1.0 DNA starboard were used in the study of SYPEITHI. Phyre2 and other bioinformatics softwares were used to analyze the physicochemical properties, antigenic epitopes, transmembrane regions, secondary and tertiary structures of EgTK. Results the EgTK gene was composed of 7 exons and 6 introns. The length of the CDS is 1 878 BP and encodes 625 amino acids. Molecular weight is 67.76ku. It is a transmembrane protein located in the cytoplasm. The secondary structure is mainly 偽 helix. The irregular curl. EgTK has 16 potential B cell linear epitopes and 11 CTL cell epitopes. 13 Th cell epitopes; The similarity between the tertiary structure of EgTK and human transketol enzyme was 97%. After multiple alignment, 473 amino acid residues took part in the alignment, excluding the redundant sequences at both ends, and 195 variant sites were found to be parsimonious information sites. Conclusion the bioinformatics analysis of EgTK protein has many T cell epitopes and has good immunogenicity, which can provide theoretical basis for gene cloning and expression of EgTK protein.
【作者單位】： 青海大學(xué)醫(yī)學(xué)院病原生物學(xué)教研室;青海大學(xué)包蟲(chóng)病重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81360255)
【分類(lèi)號(hào)】：R383.33
【正文快照】： ***囊性包蟲(chóng)病(cystic echinococcosis,CE)是由細(xì)粒棘球絳蟲(chóng)(Echinococcus granulosus,Eg)的幼蟲(chóng)引起的一種在世界范圍內(nèi)廣泛流行的寄生蟲(chóng)病,常見(jiàn)的感染途徑是誤食了感染細(xì)粒棘球絳蟲(chóng)的犬體內(nèi)成蟲(chóng)排出的蟲(chóng)卵[1]。人感染后初期常多無(wú)癥狀。血清特異抗體常早于癥狀出現(xiàn),但血清學(xué)

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1 趙靜;鐘春玖;;轉(zhuǎn)酮醇酶的研究進(jìn)展(英文)[J];Neuroscience Bulletin;2009年02期

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