本文關(guān)鍵詞：創(chuàng)傷性腦損傷合并骨折動(dòng)物模型的建立與評(píng)估　出處：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年09期 　論文類型：期刊論文

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【摘要】：目的顱腦損傷與骨折愈合相關(guān)性機(jī)制研究缺乏確實(shí)可靠的動(dòng)物實(shí)驗(yàn)?zāi)Ｐ�。文章旨在建立�?chuàng)傷性腦損傷與骨折愈合相關(guān)性研究的動(dòng)物模型,并進(jìn)行穩(wěn)定性評(píng)估。方法選擇新西蘭兔48只隨機(jī)分為4組,每組12只:對(duì)照組(不做任何處理)、單純顱腦外傷組(單純顱腦損傷模型)、單純骨折組(單純制作右側(cè)脛骨骨折模型)、腦外傷合并骨折組(制作顱腦損傷合并骨折模型)。觀察各組新西蘭兔神經(jīng)行為學(xué)變化,并術(shù)后不同時(shí)間觀察骨折愈合情況,行骨痂體積測量及病理學(xué)觀察。結(jié)果單純顱腦外傷組術(shù)后6 h、7 d、1個(gè)月神經(jīng)功能量表(NSS)評(píng)分[(9.32±0.68)、(8.01±0.56)、(7.75±0.66)分]均顯著低于對(duì)照組[(11.81±0.55)、(12.07±0.42)、(12.02±0.38)分],差異有統(tǒng)計(jì)學(xué)意義(P0.05)。與單純骨折組術(shù)后1個(gè)月周圍骨痂的體積比較,腦外傷合并骨折組明顯增大[(1.19±0.12)cm~3vs(1.81±0.13)cm~3,P0.05]。腦外傷合并骨折組術(shù)后4周,骨折局部骨痂中骨小梁顯著增多;術(shù)后8周骨小梁變得更加成熟。結(jié)論創(chuàng)傷性腦損傷合并骨折動(dòng)物模型,穩(wěn)定性及可重復(fù)性好,達(dá)到了腦損傷合并骨折相關(guān)研究所需的動(dòng)物模型標(biāo)準(zhǔn)。
[Abstract]:Objective to establish an animal model of the relationship between traumatic brain injury and fracture healing in order to establish a reliable animal model for the study of the mechanism of craniocerebral injury and fracture healing. Methods 48 New Zealand rabbits were randomly divided into 4 groups, 12 in each group: control group (without any treatment), simple craniocerebral injury group (simple craniocerebral injury model). Simple fracture group (simple to make right tibial fracture model, brain injury combined with fracture group (craniocerebral injury combined with fracture model). To observe the changes of neurobehavioral changes in each group of New Zealand rabbits. The healing of fracture was observed at different time after operation, the volume of callus was measured and the pathological observation was made. Results the neurological function scale (NSS) was used in patients with craniocerebral trauma at 6 hours and 7 days after operation. [The score of 9.32 鹵0.68 (8.01 鹵0.56) was significantly lower than that of the control group (7.75 鹵0.66). [(11.81 鹵0.55) and 12.07 鹵0.42 (12.02 鹵0.38). The difference was statistically significant (P 0.05). Compared with the simple fracture group, the volume of peripheral callus was significantly larger in the group of traumatic brain injury with fracture than that in the group of simple fracture 1 month after operation. [The trabeculae in the local callus increased significantly 4 weeks after operation in the traumatic brain injury combined with fracture group (1.81 鹵0.13 cm ~ (-3) P 0.05). Conclusion the animal model of traumatic brain injury combined with fracture is stable and reproducible, which meets the standard of animal model for the study of brain injury associated with fracture.
【作者單位】： 新疆醫(yī)科大學(xué)第一附屬醫(yī)院骨科;
【基金】：新疆維吾爾自治區(qū)自然科學(xué)基金(2014211C045)
【分類號(hào)】：R-332;R651.15
【正文快照】： 0 引言 診療過程中經(jīng)常發(fā)現(xiàn)大多數(shù)有骨折同時(shí)合并顱腦外傷的患者,其骨折局部新生骨痂的增長速度顯著加快,并且骨痂超常生長,甚至肌肉組織中會(huì)呈現(xiàn)出異位骨化的現(xiàn)象,影響患者康復(fù)及功能鍛煉[1-4]。1968年Roberts[5]首次報(bào)道表明腦外傷后身體局部會(huì)表現(xiàn)出異位骨化的現(xiàn)象,異位骨，

本文編號(hào)：1401472