本文關(guān)鍵詞：超聲評(píng)價(jià)法優(yōu)化三氯化鐵誘導(dǎo)大鼠頸總動(dòng)脈血栓模型的研究　出處：《中國(guó)神經(jīng)精神疾病雜志》2017年02期 　論文類型：期刊論文

  更多相關(guān)文章： 動(dòng)物模型 三氯化鐵誘導(dǎo)血栓 閉塞血栓 超聲評(píng)價(jià)

【摘要】：目的探討采用連續(xù)超聲評(píng)價(jià)法優(yōu)化不同三氯化鐵濃度梯度和作用時(shí)間誘導(dǎo)大鼠頸總動(dòng)脈血栓模型,為溶栓及取栓研究提供合適的在體血栓模型。方法選取20只(Sprague Dawley)SD大鼠,每組5只,分別采用20%、30%、40%、50%四種三氯化鐵濃度外敷大鼠單側(cè)頸總動(dòng)脈,使用14 L高頻超聲探頭,分別在外敷10min、15 min、20 min三個(gè)時(shí)間點(diǎn)觀察血管二維超聲圖像及彩色多普勒血流情況。選取血管閉塞最佳組大鼠,于干預(yù)120 min后觀察血管自發(fā)再通率及靜脈注射尿激酶后的血管再通率。實(shí)驗(yàn)結(jié)束后,取出干預(yù)血管甲醛固定,石蠟包埋,切片,進(jìn)行HE染色病理分析。結(jié)果三氯化鐵外敷大鼠頸總動(dòng)脈20 min、50%濃度組血管閉塞率100%,40%、30%、20%濃度組外敷20 min血管閉塞率分別20%、0%、0%(P0.001)。外敷120 min后,50%濃度組組血管自發(fā)再通率0%、40%、30%、20%組血管自發(fā)再通率100%(P0.001),50%三氯化鐵濃度造模組尾靜脈注射尿激酶后血管部分再通率為40%。50%濃度組鏡下病理顯示血栓結(jié)構(gòu)致密,尿激酶干預(yù)后管腔部分再通,血栓結(jié)構(gòu)松散。結(jié)論采用50%三氯化鐵濃度外敷大鼠頸總動(dòng)脈20 min,可能是在體血栓模型形成的有效條件,該動(dòng)物模型可能適合溶栓研究。
[Abstract]:Objective to study the continuous ultrasound evaluation method to optimize the rat common carotid artery thrombosis model with different ferric chloride concentration gradient and time of action. Methods Twenty Sprague Sprague Dawley)SD rats were selected in each group of 5 rats. 50% four kinds of ferric trichloride were applied to unilateral common carotid artery of rats with 14 L high frequency ultrasound probe for 10 minutes and 15 min respectively. Two dimensional ultrasound images and color Doppler flow were observed at three time points of 20 min. The best group of rats with vascular occlusion was selected. The spontaneous recanalization rate of blood vessel and the rate of vascular recanalization after intravenous injection of urokinase were observed after 120 min of intervention. After the experiment, the intervention vessel was fixed with formaldehyde, embedded in paraffin and sectioned. Results the vascular occlusion rate was 100% and 40% in the group of 20 min ~ 50% concentration of ferric chloride applied on the common carotid artery of rats. In the 20% group, the vascular occlusion rate for 20 min was 20 and the spontaneous recanalization rate was 0% in the 50% group after external application for 120 min. The spontaneous recanalization rate of blood vessels in 20% group was 100% (P 0.001). The vascular recanalization rate of the 50% ferric chloride concentration group was 40. 50% after urokinase was injected into the tail vein. The thrombus structure was dense and the lumen was partly recanalization after urokinase intervention in the group of 40. 50% concentration of ferric chloride. Conclusion the application of 50% ferric chloride to the common carotid artery for 20 min may be an effective condition for the formation of thrombus in vivo, and the animal model may be suitable for thrombolytic study.
【作者單位】： 北京大學(xué)第一醫(yī)院;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):H0906)
【分類號(hào)】：R-332;R543.4
【正文快照】： 1990年,KURZ等[1],將三氯化鐵外敷于大鼠頸總動(dòng)脈誘導(dǎo)閉塞性血栓形成,同時(shí)發(fā)現(xiàn)血管閉塞時(shí)間依賴于三氯化鐵溶液濃度,濃度越高,閉塞性血栓形成越快,濃度越低,閉塞血栓形成越慢。此方法形成的血栓為富含血小板、纖維蛋白和紅細(xì)胞的混合型血栓,與臨床自發(fā)性血栓組織形態(tài)學(xué)特征相，

本文編號(hào)：1401398