本文關(guān)鍵詞：人偏肺病毒減毒株免疫保護效果評估　出處：《重慶醫(yī)科大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： BALB/c小鼠 人偏肺病毒減毒突變株(M_2) 保護效果

【摘要】：目的明確人偏肺病毒減毒突變株(human metapneumovirus attenuated strain ,M_2)的免疫原性及保護效果,評價其做為疫苗的應(yīng)用前景,為hMPV減毒活疫苗的開發(fā)奠定堅實理論基礎(chǔ)。 方法清潔級6～7周齡、雌性BALB/c小鼠144只隨機分為3個組,分別為DMEM組、M_2組和WT組,每組48只。WT組每只小鼠鼻腔滴入10~7PFU/ml NL/1/00-gfp株hMPV病毒液100μl。M_2組每只小鼠鼻腔滴入10~7PFU/ml M_2減毒突變株病毒液100μl,DMEM組小鼠鼻腔滴入100μl DMEM培養(yǎng)基,滴鼻后第5、14、28、56天麻醉并處死每組動物各8只。3組分別在首次滴鼻后28、56天再次經(jīng)鼻腔滴入10~7PFU/ml NL/1/00-gfp株hMPV病毒液100μl。并于再次滴鼻后第5天處死動物。每次每組各8只。均收集血清和肺組織用于檢測血清中和抗體滴度、肺組織病毒滴度及病理改變。 結(jié)果M_2組與WT組相比,M_2毒力減弱,肺內(nèi)復(fù)制時間縮短,但所誘導(dǎo)的中和抗體滴度水平與野生型相似,且對兩亞型流行株具有交叉中和活性。平均抗NL/1/00血清抗體滴度水平高于平均抗NL/1/99。高效穩(wěn)定的抗體水平產(chǎn)生完整的下呼吸道免疫保護作用。 結(jié)論M_2作為hMPV減毒活疫苗候選疫苗,具有很好的應(yīng)用前景。
[Abstract]:Objective to identify the attenuated human metapneumovirus attenuated strain of human metapulmonary virus. The immunogenicity and protective effect of M _ 2) and its application prospect as vaccine were evaluated to lay a solid theoretical foundation for the development of live attenuated hMPV vaccine. Methods 144 female BALB/c mice of 6 and 7 weeks old were randomly divided into three groups: DMEM group (group 2) and group WT (group B). WT group (n = 48). WT group (n = 48) was dripped into the nasal cavity of 10 ~ 7 PFU / ml / mouse. The NL/1/00-gfp strain hMPV virus solution was 100 渭 l in the nasal cavity of each mouse in 10 ~ 7PFU / ml M2 attenuated mutants. The mice in DMEM group were anesthetized on day 514, 2856 after nasal drip with 100 渭 l DMEM medium, and 8 animals in each group were killed respectively 28 days after the first nasal drip. 56 days again through the nasal cavity into the 10 ~ (7) PFU / ml. NL/1/00-gfp strain hMPV virus solution was 100 渭 l. The animals were killed on the 5th day after nasal drip again. Each group had 8 animals. Serum and lung tissues were collected for detection of serum neutralizing antibody titer. Viral titer and pathological changes in lung tissue. Results compared with WT group, compared with WT group, the virulence of M _ 2 was decreased and the time of lung replication was shortened, but the titer of neutralizing antibody induced by M _ (2) was similar to that of wild type. The average antiserum antibody titer of NL/1/00 was higher than that of the average anti-NL / 1 / 99. The high and stable antibody level produced complete lower respiratory tract immunity protection. Function. Conclusion as a candidate vaccine for hMPV attenuated live vaccine, M _ (2) has a good prospect of application.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R392

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