本文關(guān)鍵詞：環(huán)境脅迫下大蹼鈴蟾抗內(nèi)毒素活性肽分子特征及臨床前期研究　出處：《昆明醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 感染 抗菌肽 大蹼鈴蟾 內(nèi)毒素 休克

【摘要】：感染是引起人類死亡的最主要原因之一,盡管目前針對細(xì)菌有許多藥物可進(jìn)行針對性的殺滅治療,但是對于感染后內(nèi)毒素引起的膿毒血癥卻缺乏有效的治療方法。內(nèi)毒素(endotoxin)是革蘭氏陰性菌細(xì)胞壁外膜中的重要組成部分,內(nèi)毒素一旦入血或大量釋放將會引起一系列瀑布效應(yīng),導(dǎo)致各個器官功能受損、免疫力下降而導(dǎo)致病人死亡。目前針對內(nèi)毒素引起的感染性休克臨床上更多的采用糖皮質(zhì)激素保護(hù)器官功能,缺乏更進(jìn)一步的主動對抗措施。大蹼鈴蟾(Bombina maxima)是我國的特有物種,由于其生存環(huán)境較為惡劣,容易受到細(xì)菌及紫外線等生物物理條件刺激,其皮膚產(chǎn)生的分泌物種類成分復(fù)雜,具有很高的研究價值。本課題利用大蹼鈴蟾作為研究對象,通過細(xì)菌及其內(nèi)毒素刺激,鑒定皮膚分泌物中誘導(dǎo)產(chǎn)生的具有中和對抗內(nèi)毒素作用的成分并探討其作用機(jī)理,蟾皮膚分泌物中持續(xù)出現(xiàn)的抗菌肽組分在宿主接觸環(huán)境微生物早期時發(fā)揮作用,提示皮膚受到脅迫后通過分泌抗菌肽迅速控制病原微生物侵人。同時研究發(fā)現(xiàn),后天免疫作為機(jī)體更為有效、多樣的保護(hù)機(jī)制,需要先天免疫效應(yīng)因子的激活。通過收集細(xì)菌脅迫后蟾皮膚分泌物進(jìn)行電泳發(fā)現(xiàn),蟾皮膚分泌物中含有KB因子,從而證實蟾皮膚抗菌膚的表達(dá)通路是由Toll樣受體(TLRs)介導(dǎo)的,而TLRs在連接先天免疫與后天免疫的關(guān)鍵環(huán)節(jié)中發(fā)揮重要作用。
[Abstract]:Infection is one of the major causes of human death, although there are many drugs for bacteria to kill the treatment of sepsis caused by the infection, but after endotoxin is the lack of effective treatment. Endotoxin (endotoxin) is an important component of outer membrane in gram negative bacteria, endotoxin into the blood or once a large number of release will trigger a series of cascade effect, leading to organ dysfunction, decreased immunity and resulting in the death of the patient. The endotoxin induced septic shock clinically more use of glucocorticoids to protect organ function, the lack of further active countermeasures. Toad (Bombina maxima) is a species endemic to China and because of their living environment is very bad, vulnerable to bacterial stimulation and UV biophysical conditions, the type of skin secretions produced Complex components, has high research value. The subject of the use of Bombina maxima as the research object, through the bacteria and endotoxin stimulation induced identification in the skin secretion with neutralization against endotoxin components and investigate its mechanism, persistent frog skin secretions of the antimicrobial peptide components in the host environment early microbial contact when prompted by the skin play a role in stress by the secretion of antimicrobial peptide rapid control of pathogenic microorganism invasion. At the same time, the study found that as the body acquired immunity is more effective, and various protection mechanisms, activate innate immune effectors. By collecting bacterial stress after frog skin secretions were found containing KB electrophoresis, factor of toad skin secretion in order to confirm the expression of antibacterial peptides, pathway of toad skin is composed of Toll like receptor (TLRs) mediated, TLRs in innate immunity and acquired connection It plays an important role in the key link of immunization.

【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R3411

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本文編號：1384709