本文關(guān)鍵詞：血清不耐熱物質(zhì)對(duì)噬菌體PL39殺滅銅綠假單胞菌的影響　出處：《中國(guó)病原生物學(xué)雜志》2017年07期 　論文類型：期刊論文

  更多相關(guān)文章： 銅綠假單胞菌 噬菌體 人血清 小鼠血清 不耐熱物質(zhì)

【摘要】：目的觀察人血清和鼠血清中不耐熱物質(zhì)對(duì)噬菌體PL39殺滅不同克隆銅綠假單胞菌的影響。方法采用雙層平板法篩選PL39敏感宿主菌;根據(jù)ERIC-PCR圖譜分析敏感宿主菌的遺傳差異性。分別添加新鮮及熱滅活人血清和鼠血清,觀察其對(duì)PL39殺滅不同敏感宿的影響。結(jié)果篩選到9株P(guān)L39敏感宿主菌,其中2株菌ERIC指紋圖譜無(wú)明顯不同,其他菌株的指紋圖譜明顯不同。9株菌均能完全抵抗人血清和鼠血清的溶菌作用;噬菌體PL39在兩種血清中均能保持恒定的成斑活性,但在人血清中對(duì)9株宿主菌的殺滅作用均被抑制;在小鼠血清中,PL39對(duì)L39菌有一定殺滅作用,對(duì)其他宿主菌無(wú)殺滅作用;兩種血清熱滅活(56℃,30min)后對(duì)PL39殺滅不同宿主菌作用無(wú)顯著影響,與營(yíng)養(yǎng)肉湯中的PL39殺菌作用相比差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論人血清和鼠血清中的不耐熱物質(zhì)均可影響噬菌體PL39對(duì)其宿主菌的殺滅作用。
[Abstract]:Objective to observe the effect of heat-resistant substances in human serum and mouse serum on bacteriophage PL39 killing Pseudomonas aeruginosa clones from different clones. The genetic diversity of sensitive host bacteria was analyzed according to ERIC-PCR map. Fresh and heat-inactivated human serum and mouse serum were added respectively. The results showed that 9 strains of PL39 sensitive host bacteria were screened, of which 2 strains had no significant difference in ERIC fingerprints. The fingerprinting of other strains was obviously different. 9 strains could completely resist the bacteriolytic effect of human serum and mouse serum. Bacteriophage PL39 could maintain constant plaque formation activity in both sera, but its killing activity against 9 host strains in human serum was inhibited. In the serum of mice, PL39 had a certain killing effect on L39 bacteria, but no killing effect on other host bacteria. There was no significant effect on the killing of different host bacteria by PL39 after two kinds of serum heat inactivation (56 鈩，

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