本文關(guān)鍵詞：針灸對環(huán)磷酰胺模型荷瘤小鼠骨髓細胞DNA修復相關(guān)蛋白動態(tài)調(diào)節(jié)的研究　出處：《中華中醫(yī)藥雜志》2016年08期 　論文類型：期刊論文

  更多相關(guān)文章： 針灸 環(huán)磷酰胺 荷瘤小鼠 骨髓抑制 白細胞減少

【摘要】：目的:觀察針灸對環(huán)磷酰胺(CTX)化療后的荷瘤小鼠白細胞及骨髓細胞DNA修復蛋白O6-甲基鳥嘌呤-DNA甲基轉(zhuǎn)移酶。(MGMT)、DNA聚合酶β(POLβ)、切除修復交叉互補基因(XPD)表達的影響。方法:160只雄性清潔級昆明種小鼠植種S180肉瘤,按體質(zhì)量隨機分為荷瘤空白(A)組、荷瘤模型(B)組、荷瘤針刺(C)組、荷瘤艾灸(D)組。B、C、D組腹腔注射CTX 150mg/kg制備CTX荷瘤模型小鼠,A組注射同等劑量的0.9%氯化鈉溶液。C、D組分別選取"大椎""膈俞""腎俞""足三里"進行治療,A、B組每日陪同抓取固定,不治療。鏡檢各組小鼠外周血白細胞,免疫組化法檢測骨髓細胞DNA修復蛋白MGMT、POLβ、XPD的表達情況,統(tǒng)計分析數(shù)據(jù)。結(jié)果:針灸可提升化療后荷瘤小鼠白細胞數(shù)量(P0.05),亦能上調(diào)CTX化療后荷瘤小鼠骨髓細胞DNA修復相關(guān)蛋白MGMT、POLβ、XPD的表達。結(jié)論:針灸升高CTX化療后荷瘤小鼠外周血白細胞的療效確切,其升白機制與上調(diào)CTX化療后荷瘤小鼠骨髓細胞DNA修復相關(guān)蛋白MGMT、POLβ、XPD的表達及改善化療后骨髓抑制關(guān)系密切。
[Abstract]:Objective: to observe the effect of acupuncture and moxibustion on DNA repair protein O6-methylguanine-DNA methyltransferase (O6-methylguanine-DNA methyltransferase) in leukocytes and bone marrow cells of tumor-bearing mice after chemotherapy with cyclophosphamide (CTX). DNA polymerase 尾 -POL 尾, excision and repair of cross complementary gene (XPD) expression. Methods S180 sarcoma was implanted in 1: 160 male Kunming mice of clean grade. According to body mass, they were randomly divided into three groups: group A, group B), group C (acupuncture), group D (moxibustion). Group D was intraperitoneally injected with CTX 150mg / kg to make CTX tumor bearing model mice. Group A was injected with the same dose of 0.9% sodium chloride solution. Group D selected "Dazhui", "Geshu", "Shenshu" and "Zusanli" respectively to carry out the treatment of AZA B group daily accompanied by grab fixation, no treatment. Microscopic examination of peripheral blood leukocytes of mice in each group. The expression of DNA repair protein (MGMT-POL 尾 -XPD) in bone marrow cells was detected by immunohistochemistry. Results: acupuncture and moxibustion could increase the number of leukocytes in tumor-bearing mice after chemotherapy and up-regulate the MGMT of DNA repair related protein in bone marrow cells of tumor-bearing mice after chemotherapy with CTX. Conclusion: acupuncture and moxibustion can increase the leucocyte expression in peripheral blood of tumor-bearing mice after CTX chemotherapy. The mechanism of leukopenia was closely related to the up-regulation of the expression of DNA repair related protein MGMT-POL 尾 -XPD in bone marrow cells of tumor-bearing mice after chemotherapy and the improvement of bone marrow suppression after chemotherapy.
【作者單位】： 河南中醫(yī)藥大學;靈寶市第一人民醫(yī)院;鄭州大學基礎(chǔ)醫(yī)學院;河南中醫(yī)藥大學第一附屬醫(yī)院;
【基金】：國家自然科學基金重大計劃項目(No.90709035)~~
【分類號】：R245;R-332
【正文快照】： Research on effects of acupuncture and moxibustion on DNA excision repair-relatedproteins of bone marrow cell in cyclophosphamide-induced tumor-bearing miceLU Mei1,WANG Jia-li2,CAO Da-ming1,LI Dao-ming3,ZHAO Xi-xin1,LI Jian-wei4,YU Dong-dong1,TENG Ying-c

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