本文關(guān)鍵詞：尿酸排泄不良型高尿酸血癥動(dòng)物模型的建立　出處：《中國(guó)比較醫(yī)學(xué)雜志》2017年08期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 高尿酸血癥 氧嗪酸鉀 吡嗪酰胺 乙胺丁醇 大鼠動(dòng)物模型

【摘要】：目的探討構(gòu)建尿酸排泄不良型高尿酸血癥大鼠模型的可靠方法,為尿酸排泄不良型高尿酸血癥發(fā)病機(jī)制的研究及治療方案的優(yōu)化奠定基礎(chǔ)。方法采用氧嗪酸鉀(300 mg/kg)分別與吡嗪酰胺(300 mg/kg)、乙胺丁醇(250 mg/kg)聯(lián)合造模。連續(xù)給藥1周、3周、5周,檢測(cè)大鼠血、尿、便中尿酸水平,同時(shí)檢測(cè)肝、腎功能,并進(jìn)行組織學(xué)病理切片觀察。結(jié)果氧嗪酸鉀與乙胺丁醇聯(lián)合造模組大鼠血尿酸出現(xiàn)先升高后降低的現(xiàn)象,而氧嗪酸鉀與吡嗪酰胺聯(lián)合造模組在連續(xù)給藥期間血尿酸和尿液中尿酸升高平穩(wěn)。兩種方法對(duì)肝臟、腎臟均無(wú)明顯損害。結(jié)論氧嗪酸鉀與吡嗪酰胺聯(lián)合能有效建立尿酸排泄不良型高尿酸血癥大鼠模型,且模型穩(wěn)定性好,其發(fā)病過(guò)程更符合臨床尿酸排泄不良型高尿酸血癥的病程。
[Abstract]:Objective to explore the method of constructing reliable uric acid excretion adverse type hyperuricemia rat model, and lay the foundation for the optimization of treatment and pathogenesis of hyperuricemia uric acid excretion adverse type. Method using potassium oxonate (300 mg/kg) and pyrazinamide (300 mg/kg), ethambutol (250 mg/kg) combined with continuous administration model. 1 weeks, 3 weeks, 5 weeks, rats were detected in blood, urine, feces and uric acid, renal function, and detection of liver histological pathological observation. The potassium oxonate and ethambutol combined model rats serum uric acid increased first and then decreased, while the potassium oxonate and combined with pyrazinamide model group continuously during the administration of blood uric acid and urine uric acid increased steadily. The two methods on the liver and kidneys were no significant damage. Conclusion potassium oxonate combined with pyrazinamide can effectively establish uric acid excretion bad high uric acid in the blood The model of the rat, and the model is stable, is more consistent with the course of hyperuricemia in clinical uric acid excretion.

【作者單位】： 遼寧中醫(yī)藥大學(xué);遼寧中醫(yī)藥大學(xué)附屬醫(yī)院;
【基金】：遼寧省衛(wèi)計(jì)委資助項(xiàng)目(LNCCC-D54-2015) 沈陽(yáng)市科技計(jì)劃項(xiàng)目(F15-139-9-39)
【分類(lèi)號(hào)】：R-332;R589.7
【正文快照】： 尿酸是嘌呤代謝的最終產(chǎn)物,由于人類(lèi)的尿酸酶基因在進(jìn)化過(guò)程中發(fā)生了突變,缺乏尿酸酶,嘌呤代謝最終大部分以尿酸形式排出,這使得人類(lèi)的血尿酸水平遠(yuǎn)遠(yuǎn)高于其他哺乳動(dòng)物。而實(shí)驗(yàn)常用的嚙齒類(lèi)動(dòng)物,因?yàn)榫哂心蛩崦?可將尿酸降解為尿囊素后排出體外[1]。因此,為了使動(dòng)物模型與人類(lèi)，

本文編號(hào)：1378139