發(fā)布時(shí)間：2017-12-31 11:08

  本文關(guān)鍵詞：左歸丸對(duì)D-半乳糖致老化小鼠模型認(rèn)知功能和聽功能損傷的干預(yù)作用　出處：《中國(guó)老年學(xué)雜志》2017年12期 　論文類型：期刊論文

  更多相關(guān)文章： 左歸丸 認(rèn)知功能 聽性腦干反應(yīng)

【摘要】：目的觀察左歸丸對(duì)D-半乳糖致老化小鼠認(rèn)知功能和聽功能的影響。方法將雄性C57BL/6小鼠隨機(jī)分為4組,模型組、左歸丸低劑量和高劑量組按照200 mg/kg皮下注射D-半乳糖,對(duì)照組注射等體積生理鹽水,連續(xù)造模60 d。左歸丸低劑量和高劑量組在造模的第41天開始按2 g/kg和4 g/kg的劑量連續(xù)灌胃左歸丸混懸液20 d。采用Morris水迷宮觀察小鼠的空間學(xué)習(xí)和記憶能力,采用聽性腦干反應(yīng)(ABR)檢測(cè)小鼠聽功能。結(jié)果 Morris水迷宮定位航行實(shí)驗(yàn)結(jié)果顯示,從訓(xùn)練的第3天開始,模型組的潛伏期明顯高于對(duì)照組,左歸丸低劑量和高劑量組的潛伏期均低于模型組�？臻g探索實(shí)驗(yàn)結(jié)果顯示,模型組穿越平臺(tái)次數(shù)和目標(biāo)象限時(shí)間百分比明顯低于對(duì)照組(P0.01,P0.05);左歸丸低劑量組和高劑量組的穿越平臺(tái)次數(shù)和目標(biāo)象限百分比均高于模型組,其中高劑量組與模型組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。ABR結(jié)果顯示,在8 k Hz和16 k Hz時(shí)模型組聽閾值明顯高于對(duì)照組,左歸丸低劑量組和高劑量組聽閾值均低于模型組(P0.05,P0.01)。結(jié)論左歸丸對(duì)D-半乳糖導(dǎo)致的聽功能損傷和認(rèn)知功能下降有保護(hù)作用。
[Abstract]:Objective To observe the effect of Zuogui Pill on D- galactose induced aging mice cognitive function and auditory function effects. Methods male C57BL/6 mice were randomly divided into 4 groups, model group, Zuoguiwan group with low dose and high dose 200 mg/kg subcutaneous injection of D- galactose, the control group were given normal saline, spatial learning and memory the ability of continuous model 60 D. Zuoguiwan low dose and high dose group made in the forty-first day at 2 g/kg and 4 g/kg dose by intragastric administration of Zuogui pill suspension 20 D. mice were observed by Morris water maze, the auditory brainstem response (ABR) test in mouse auditory function. Results Morris water maze navigation experiment results show that from the beginning of training for third days, the model group was significantly higher than the control group, Zuoguiwan low dose and high dose group were significantly lower than in the model group. The experimental results show that the exploration of space, time and number of crossing platform model group The percentage of target quadrant was significantly lower than the control group (P0.01, P0.05); Zuogui pill low dose group and high dose group the number of crossing platform and target quadrant percentage were higher than that of model group, which was statistically significant difference between high dose group and model group (P0.05).ABR results showed that in 8 K Hz and 16 K Hz when listening to the threshold of model group was significantly higher than the control group, Zuogui pill low dose group and high dose group thresholds were lower than the model group (P0.05, P0.01). Conclusion Zuogui Pill on D- galactose induced damage of auditory function and cognitive decline has a protective effect.

【作者單位】： 上海中醫(yī)藥大學(xué)教學(xué)實(shí)驗(yàn)中心;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81102695) 上海市教委科研創(chuàng)新項(xiàng)目(14YZ064) 上海市中西醫(yī)結(jié)合重點(diǎn)學(xué)科建設(shè)資助項(xiàng)目(J50301)
【分類號(hào)】：R285.5;R-332
【正文快照】： 大量的研究資料顯示,老年性聾與認(rèn)知功能下降有密切關(guān)聯(lián),并且常常伴隨發(fā)生[1~4]。依據(jù)中醫(yī)理論,“腎主耳”,“腎主骨生髓…腦為髓�！�,腎精虧虛是老年性聾和老年性癡呆的重要病機(jī),而補(bǔ)腎填精是治療老年性聾和老年性癡呆的重要治則[5~7]。左歸丸是補(bǔ)腎填精的經(jīng)典代表方劑。該方

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