本文關(guān)鍵詞：依達(dá)拉奉對(duì)糖尿病角膜神經(jīng)病變大鼠角膜神經(jīng)的保護(hù)作用　出處：《重慶醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 依達(dá)拉奉 糖尿病角膜神經(jīng)病變 角膜神經(jīng) 氧化應(yīng)激 發(fā)病機(jī)制

【摘要】：背景隨著糖尿病患者的日益增加，糖尿病性角膜病變?cè)絹?lái)越受到人們的重視，導(dǎo)致糖尿病角膜病變發(fā)病的主要原因?yàn)樘悄虿∫鸬慕悄ど窠?jīng)病變，因此探討糖尿病角膜神經(jīng)病變的具體發(fā)病機(jī)制具有重要意義。 目的探討新型自由基清除劑依達(dá)拉奉對(duì)糖尿病角膜神經(jīng)病變大鼠角膜神經(jīng)的保護(hù)作用，研究糖尿病角膜神經(jīng)病變發(fā)病機(jī)制中氧化應(yīng)激的作用。 方法健康SD雄性大鼠70只，隨機(jī)選取20只不做任何處理，作為正常對(duì)照組；其余50只采用一次性腹腔注射鏈脲佐菌素（STZ）（60mg/kg）誘導(dǎo)建立糖尿病大鼠模型，成模后選取血糖值15mmol/L的40只大鼠應(yīng)用隨機(jī)數(shù)字表法隨機(jī)分為依達(dá)拉奉治療組（依達(dá)拉奉組）和生理鹽水對(duì)照組（生理鹽水組），依達(dá)拉奉組每天給予自制的0.2g/L依達(dá)拉奉滴眼液點(diǎn)眼，生理鹽水組每天給予生理鹽水點(diǎn)眼，，每次1滴，每日3次，預(yù)防性用藥直至實(shí)驗(yàn)動(dòng)物被處死，分別于造模成功后的第6、8、10、12周每組選取5只大鼠，檢測(cè)角膜知覺后處死大鼠，觀察角膜神經(jīng)形態(tài)、角膜神經(jīng)纖維數(shù)量、角膜組織中丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。 結(jié)果1.角膜知覺檢測(cè)：生理鹽水組大鼠自造模6周起出現(xiàn)角膜知覺的減退，第12周時(shí)角膜知覺減退明顯；依達(dá)拉奉組第6周時(shí)角膜知覺未見明顯減退，第8、10、12周角膜知覺較正常對(duì)照組減退，但較生理鹽水組提高。 2.角膜神經(jīng)乙酰膽堿酯酶（AChE）染色及形態(tài)學(xué)觀察：生理鹽水組自造模后6周起，角膜基質(zhì)神經(jīng)干變細(xì)、分支減少，上皮下神經(jīng)網(wǎng)叢密度稀疏、神經(jīng)活性降低，且隨病程延長(zhǎng)上述變化日益明顯；依達(dá)拉奉組較生理鹽水組神經(jīng)形態(tài)學(xué)明顯改善，且神經(jīng)活性提高。 3.角膜神經(jīng)纖維計(jì)數(shù)：生理鹽水組大鼠自成模后第6周起，出現(xiàn)角膜神經(jīng)纖維數(shù)量減少，且隨病程延長(zhǎng)，角膜神經(jīng)纖維數(shù)量減少加劇；依達(dá)拉奉組與生理鹽水組相比，角膜神經(jīng)纖維數(shù)量明顯增多，差異有統(tǒng)計(jì)學(xué)意義（p0.01）。 4.角膜組織中MDA含量及SOD活性變化：生理鹽水組大鼠角膜組織自造模6周起出現(xiàn)MDA含量升高，SOD活性降低，隨病程延長(zhǎng)變化日益明顯；依達(dá)拉奉組與生理鹽水組比較，角膜組織中MDA含量下降，SOD活性提高，差異有統(tǒng)計(jì)學(xué)意義（p0.01）。 結(jié)論1.依達(dá)拉奉可降低糖尿病角膜神經(jīng)病變所致的角膜神經(jīng)損傷，對(duì)神經(jīng)形態(tài)學(xué)及功能學(xué)起保護(hù)作用； 2.氧化應(yīng)激在糖尿病角膜神經(jīng)病變的發(fā)病機(jī)制占有重要地位。
[Abstract]:With the background of diabetes is increasing, diabetic keratopathy has attracted more and more attention, mainly due to the incidence of corneal neuropathy of diabetic keratopathy is caused by diabetes, so to discuss the pathogenesis of diabetic corneal neuropathy has important significance.
Objective to investigate the protective effect of edaravone, a new free radical scavenger, on the corneal nerves in diabetic rats with corneal neuropathy, and to study the role of oxidative stress in the pathogenesis of diabetic corneal neuropathy.
Methods 70 male SD rats, 20 rats were randomly selected without any treatment, as the control group; the remaining 50 by intraperitoneal injection of streptozotocin (STZ) (60mg/kg) induced diabetic rats were induced into the mold after the selection of blood glucose 15mmol/L 40 rats using random number table randomly divided into edaravone group (edaravone group) and saline control group (saline group) and edaravone group were given edaravone 0.2g/L eyedrops and self-made, saline group were given saline drops, 1 drops each time, 3 times daily, preventive medication until the animal was killed, were made after the success of the model 6,8,10,12 weeks of each group 5 rats were selected to detect corneal sensitivity after the rats were sacrificed and the observation of corneal nerve morphology, number of corneal nerve fibers in the corneal tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) Activity.
Results 1. corneal perception test: saline group rats from the model 6 week decline in corneal sensitivity, corneal sensitivity decreased significantly at week twelfth; edaravone group sixth weeks no obvious loss of corneal sensation, 8,10,12 week corneal sensitivity compared with the normal control group decreased, but compared with the saline group.
2. acetylcholinesterase (AChE) staining and corneal nerve morphology: saline group from 6 weeks after operation, corneal stromal neural stem thinning, reduced branches, subepithelial nerve net plexus nerve activity decreased, sparse density, and with the extension of the duration of these changes is more and more obvious; the edaravone group compared with the saline group of nerve morphology significantly improved and the neural activity increased.
3. corneal nerve fiber count: saline group rats into sixth weeks after die, the number of corneal nerve fibers decreased, and with the extension of the course, reduce the number of corneal nerve fibers increased; the edaravone group compared with the saline group, the number of corneal nerve fibers increased significantly, the difference was statistically significant (P0.01).
The change of MDA content and SOD activity of 4. in corneal tissue: physiological saline group rat corneal tissue from the model 6 week MDA content increased, SOD activity decreased with the extension of the course change is more and more obvious; the edaravone group compared with the saline group, MDA decreased in corneal tissue, the activity of SOD increased, the difference was statistically significant (P0.01).
Conclusion edaravone can reduce the injury of corneal nerve 1. diabetic corneal neuropathy caused by the neural morphological and functional study of the protective effect;
2. oxidative stress plays an important role in the pathogenesis of diabetic corneal neuropathy.

【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R587.1;R774.1;R-332

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