玉米抗灰斑病主效QTL發(fā)掘及其精細(xì)定位
發(fā)布時(shí)間：2018-09-10 20:40
【摘要】：由尾孢菌侵染玉米葉片引起的玉米灰斑病已成為我國(guó)東華北及西南玉米產(chǎn)區(qū)重要的病害之一,發(fā)掘抗灰斑病基因并應(yīng)用于抗病品種選育是經(jīng)濟(jì)有效的防控途徑。本文首先通過(guò)收集并整理玉米抗灰斑病QTL文獻(xiàn),利用元分析方法進(jìn)行整合,確定"一致性"抗病QTL;然后以CN165為供體親本和81162為受體親本構(gòu)建的片段代換系群體,進(jìn)行QTL驗(yàn)證,發(fā)掘主效QTL位點(diǎn);再篩選含有目標(biāo)區(qū)段的片段代換系構(gòu)建分離群體,進(jìn)行QTL精細(xì)定位。取得的主要研究結(jié)果如下:利用元分析方法分析并整理了 14篇玉米抗灰斑病QTL文獻(xiàn)的信息,共篩選確定了13個(gè)一致性QTL區(qū)間,分布在玉米第1、2、4、7、8號(hào)染色體上。利用以自交系81162為輪回親本、自交系CN165為非輪回親本構(gòu)建的回交導(dǎo)入群體根據(jù)連鎖不平衡原理對(duì)13個(gè)一致性QTL進(jìn)行驗(yàn)證,在13個(gè)一致性QTL區(qū)間共獲得20個(gè)偏分離位點(diǎn)。第1和第4染色體上偏分離最嚴(yán)重,其他染色體上偏分離度較小。說(shuō)明第1和第4染色體上存在著效應(yīng)較大的抗病QTL。第1染色體標(biāo)記umc2227、bnlg1832、umc1243、umc2025、umc1515、umc1297、umc1461 處供體基因頻率均在 50%以上,可能存在幾個(gè)連鎖的抗病基因。第4染色體上基因位于標(biāo)記bnlg2291和umc1 194之間。結(jié)合背景檢測(cè)結(jié)果,從BC2F3抗病家系中選取3個(gè)目標(biāo)家系,與輪回親本81162雜交并自交得到目標(biāo)區(qū)段分離群體。選取來(lái)自同一自交果穗的176個(gè)單株,采用完備區(qū)間作圖法,對(duì)第4染色體的主效QTL-CNgls4進(jìn)行定位,將QTL定位于標(biāo)記umc1329-bnlg1137 之間。根據(jù)B73序列信息自主開發(fā)引物67對(duì),在目標(biāo)區(qū)段獲得多態(tài)性引物7對(duì),利用這7對(duì)多態(tài)性引物對(duì)分離群體的882個(gè)高抗和37個(gè)高感單株進(jìn)行重組體篩選,在該區(qū)間內(nèi)共找到11個(gè)重組體。通過(guò)分析,將QTL區(qū)間精細(xì)定位到標(biāo)記Snyw042和Snyw008之間,物理距離為2.19Mb,物理位置為 167,355,376bp-169,548,333bp。通過(guò)發(fā)掘自交系CN165中第4染色體的主效QTL,為選育抗玉米灰斑病的種質(zhì)材料、玉米分子標(biāo)記輔助選擇育種及抗灰斑病基因的克隆和功能分析提供理論參考。
[Abstract]:The gray spot of maize caused by the infection of cemocystis in maize leaves has become one of the most important diseases in maize growing areas in the east and southwest of China. It is an economical and effective way to explore the genes of resistance to gray spot disease and to apply it to the breeding of disease-resistant varieties. In this paper, the QTL literature on resistance to gray spot disease in maize was collected and sorted, and the "consistent" disease-resistant QTL; was determined by meta-analysis method. Then the fragment substitution line population constructed with CN165 as donor parent and 81162 as recipient parent was used for QTL verification. The dominant QTL loci were identified, and then the fragment substitution lines containing the target region were selected to construct isolated populations for QTL precise mapping. The main results were as follows: 14 papers on QTL resistance to gray spot disease in maize were analyzed and sorted by meta-analysis. Thirteen consistent QTL intervals were selected and distributed on chromosome 7 and 8 in maize. Using inbred line 81162 as recurrent parent and inbred line CN165 as non-recurrent parent, 13 consistent QTL were verified according to linkage disequilibrium principle. A total of 20 partial segregation sites were obtained in 13 consistent QTL intervals. The first and fourth chromosomes were most seriously biased, while the other chromosomes were less skewed. It is suggested that there are disease-resistant QTL. on chromosomes 1 and 4. The frequency of donor genes at umc2227,bnlg1832,umc1243,umc2025,umc1515,umc1297,umc1461 on chromosome 1 was above 50%, and there might be several linkage resistant genes. The genes on chromosome 4 are located between the labeled bnlg2291 and umc1 194. Based on the results of background test, three target families were selected from BC2F3 resistant families, crossed with recurrent parent 81162 and self-crossed to obtain the target segment segregation population. 176 individual plants from the same self-pollinated ear were selected and the main effect QTL-CNgls4 of chromosome 4 was mapped by complete interval mapping method. The QTL was mapped between labeled umc1329-bnlg1137. According to the information of B73 sequence, 67 pairs of primers were developed and 7 pairs of polymorphic primers were obtained in the target region. The 7 pairs of polymorphic primers were used to screen 882 highly resistant and 37 highly susceptible individual plants from isolated populations. A total of 11 recombinant bodies were found in this region. Through the analysis, the QTL interval was finely located between the tagged Snyw042 and Snyw008, the physical distance was 2.19 Mb, and the physical position was 167355376bp-169548333bp. The main effect QTL, of chromosome 4 in inbred line CN165 was found to be a germplasm material for breeding resistant to gray spot of maize. Molecular marker-assisted selection breeding of maize and cloning and functional analysis of genes resistant to gray spot disease were provided as theoretical references.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S435.13
，
本文編號(hào)：2235553
[Abstract]:The gray spot of maize caused by the infection of cemocystis in maize leaves has become one of the most important diseases in maize growing areas in the east and southwest of China. It is an economical and effective way to explore the genes of resistance to gray spot disease and to apply it to the breeding of disease-resistant varieties. In this paper, the QTL literature on resistance to gray spot disease in maize was collected and sorted, and the "consistent" disease-resistant QTL; was determined by meta-analysis method. Then the fragment substitution line population constructed with CN165 as donor parent and 81162 as recipient parent was used for QTL verification. The dominant QTL loci were identified, and then the fragment substitution lines containing the target region were selected to construct isolated populations for QTL precise mapping. The main results were as follows: 14 papers on QTL resistance to gray spot disease in maize were analyzed and sorted by meta-analysis. Thirteen consistent QTL intervals were selected and distributed on chromosome 7 and 8 in maize. Using inbred line 81162 as recurrent parent and inbred line CN165 as non-recurrent parent, 13 consistent QTL were verified according to linkage disequilibrium principle. A total of 20 partial segregation sites were obtained in 13 consistent QTL intervals. The first and fourth chromosomes were most seriously biased, while the other chromosomes were less skewed. It is suggested that there are disease-resistant QTL. on chromosomes 1 and 4. The frequency of donor genes at umc2227,bnlg1832,umc1243,umc2025,umc1515,umc1297,umc1461 on chromosome 1 was above 50%, and there might be several linkage resistant genes. The genes on chromosome 4 are located between the labeled bnlg2291 and umc1 194. Based on the results of background test, three target families were selected from BC2F3 resistant families, crossed with recurrent parent 81162 and self-crossed to obtain the target segment segregation population. 176 individual plants from the same self-pollinated ear were selected and the main effect QTL-CNgls4 of chromosome 4 was mapped by complete interval mapping method. The QTL was mapped between labeled umc1329-bnlg1137. According to the information of B73 sequence, 67 pairs of primers were developed and 7 pairs of polymorphic primers were obtained in the target region. The 7 pairs of polymorphic primers were used to screen 882 highly resistant and 37 highly susceptible individual plants from isolated populations. A total of 11 recombinant bodies were found in this region. Through the analysis, the QTL interval was finely located between the tagged Snyw042 and Snyw008, the physical distance was 2.19 Mb, and the physical position was 167355376bp-169548333bp. The main effect QTL, of chromosome 4 in inbred line CN165 was found to be a germplasm material for breeding resistant to gray spot of maize. Molecular marker-assisted selection breeding of maize and cloning and functional analysis of genes resistant to gray spot disease were provided as theoretical references.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S435.13
，
本文編號(hào)：2235553
本文鏈接：http://sikaile.net/shoufeilunwen/zaizhiyanjiusheng/2235553.html
最近更新
教材專著
