泰安地區(qū)兔源大腸桿菌的流行情況與耐藥性分析
發(fā)布時(shí)間：2018-07-26 16:49
【摘要】：大腸桿菌廣泛存在于人和動(dòng)物體內(nèi),是腸道中的常駐菌也是一種條件性致病菌,一部分特殊血清型的大腸桿菌對(duì)人尤其是嬰兒和動(dòng)物具有病原性。兔大腸桿菌病在臨床上多混感,是由致病性大腸埃希氏菌及其毒素所引發(fā)的具有暴發(fā)性的仔兔腸道傳染病,并表現(xiàn)出潛伏期短、死亡率高的特點(diǎn)。各個(gè)年齡段的兔子對(duì)大腸桿菌都比較易感,因此在兔養(yǎng)殖業(yè)上給其帶來的經(jīng)濟(jì)損失明顯。本試驗(yàn)主要是為了了解泰安地區(qū)兔養(yǎng)殖場大腸桿菌的流行情況與耐藥性。在2016年3月份從泰安(寧陽、新泰、東平)三個(gè)兔養(yǎng)殖場各采集20份兔子的糞便樣品,三個(gè)養(yǎng)殖場總共采集樣品60份。采集后的樣品經(jīng)過選擇培養(yǎng)基并對(duì)其進(jìn)行革蘭氏染色和16srRNA分離大腸桿菌,并采用進(jìn)行生化試驗(yàn)觀察大腸桿菌的生化特性。通過對(duì)大腸桿菌分離鑒定后,采用紙片瓊脂擴(kuò)散法(K-B法)檢測(cè)其對(duì)常用14種抗生素的敏感性可以表明兔源大腸桿菌分離菌株的耐藥性,同時(shí)亦可了解大腸桿菌其耐藥表型。之后本試驗(yàn)檢測(cè)了兔源大腸桿菌分離菌株的24種耐藥基因的存在情況。然后通過檢測(cè)細(xì)菌Ⅰ類整合子的方法對(duì)大腸桿菌分離株進(jìn)行試驗(yàn)以了解細(xì)菌的耐藥性。最后通過腸桿菌基因間重復(fù)共有序列(ERIC)PCR對(duì)兔源大腸桿菌分離菌株進(jìn)行分子的多樣性分析。本試驗(yàn)通過對(duì)大腸桿菌的分離鑒定后共分離獲得55株非重復(fù)大腸桿菌。其藥敏試驗(yàn)的結(jié)果表明,這55株大腸桿菌對(duì)試驗(yàn)所用的14種抗生素表現(xiàn)出不同程度的耐藥,敏感性各不相同。對(duì)四環(huán)素不敏感,耐藥率是試驗(yàn)中最高的,為78.2%,但對(duì)頭孢他啶、頭孢曲松、亞胺培南、阿米卡星這四種藥物表現(xiàn)為敏感,耐藥率低。耐藥基因檢測(cè)結(jié)果表明55株兔源大腸桿菌分離菌株中檢測(cè)出11種耐藥基因,其中檢出率最高的是β內(nèi)酰胺酶類的blaTEM和blaCTX-M耐藥基因,檢出率分別是blaTEM(98.18%)和blaCTX-M(94.5%),其次是磺胺類的耐藥基因sul-2(58.18%)。經(jīng)Ⅰ類整合子檢測(cè)表明在55株兔源大腸桿菌分離菌株中檢測(cè)到17株大腸桿菌攜帶Ⅰ類整合子,檢測(cè)率為30.91%。本試驗(yàn)在檢測(cè)到的Ⅰ類整合子中發(fā)現(xiàn)其共攜帶7種耐藥基因盒,分別為dfrA17+aadA5,dfrA1+catB3+aac A4,aadA2+LinF,dfrA1+aadA1,aadA22,dfrA12+orfF+addA2,aadA16+dfrA27+arr-3。ERIC-PCR把分離菌株分為23種基因型(A-W),它們的同源性在58%-100%之間。目前大腸桿菌的流行規(guī)律、特點(diǎn)及耐藥性問題是全球關(guān)注的熱點(diǎn),本試驗(yàn)分析兔養(yǎng)殖場中大腸桿菌的耐藥特點(diǎn)和整合子特點(diǎn),對(duì)泰安地區(qū)兔源大腸桿菌的耐藥性方面有了一定的認(rèn)識(shí),為臨床合理選擇抗生素提供了可以借鑒的初步依據(jù),對(duì)以后在臨床工作中能更全面的防治大腸桿菌所引發(fā)的感染,從而更有針對(duì)性的做出預(yù)防對(duì)策。
[Abstract]:Escherichia coli exists widely in human and animal body. It is a resident bacteria in intestinal tract and is also a kind of conditional pathogenic bacteria. Some special serotypes of Escherichia coli have pathogenicity to human, especially to infants and animals. Rabbit colibacillosis is a fulminant intestinal infection caused by pathogenic Escherichia coli and its toxin, which has the characteristics of short incubation period and high mortality. Rabbits of all ages are susceptible to Escherichia coli. The purpose of this study was to understand the prevalence and drug resistance of Escherichia coli in rabbit farms in Tai'an area. In March 2016, 20 rabbit feces were collected from three rabbit farms in Taian (Ningyang, Xintai and Dongping), and 60 samples were collected from three farms. The collected samples were separated by Gram staining and 16srRNA. The biochemical characteristics of Escherichia coli were observed by biochemical test. After isolation and identification of Escherichia coli, the susceptibility of Escherichia coli isolates to 14 kinds of antibiotics was detected by disk Agar diffusion method (K-B), which indicated the resistance of Escherichia coli strains isolated from rabbits, and the resistance phenotype of Escherichia coli was also known. After that, the presence of 24 resistant genes in Escherichia coli strains isolated from rabbits was detected. Then we tested the Escherichia coli isolates by detecting the class I integron of bacteria to understand the drug resistance of the bacteria. Finally, the molecular diversity of Escherichia coli isolated from rabbit was analyzed by repeated common sequence (ERIC) PCR of Enterobacter coli. After isolation and identification of Escherichia coli, 55 strains of non-repeated Escherichia coli were isolated. The results of drug sensitivity test showed that the 55 strains of Escherichia coli showed different degrees of resistance to 14 kinds of antibiotics used in the experiment, and the sensitivity was different. Tetracycline was insensitive to tetracycline, and the drug resistance rate was 78.2, but it was sensitive to ceftazidime, ceftriaxone, imipenem and amikacin, and the resistance rate was low. The results of drug resistance gene detection showed that 11 resistant genes were detected in 55 strains of Escherichia coli isolated from rabbits, among which the highest detection rate was detected by 尾 lactamases blaTEM and blaCTX-M genes. The detection rates of blaTEM (98.18%) and blaCTX-M (94.5%), followed by sulfonamides resistant gene sul-2 (58.18%). The detection of class I integron showed that 17 strains of Escherichia coli carrying class I integron were detected in 55 strains of Escherichia coli isolated from rabbits, and the detection rate was 30.91%. In this study, we found that there were 7 drug resistant gene cassettes in the class I integron, which were dfrA17 aadA5, dfrA1 catB3 aac A4A4, LinaadA2 lindfraadA1, aadA22dfrA12 orfF addA2aadA16 dfrA27 arr-3.ERIC-PCR. The isolates were divided into 23 genotypes (A-W), and their homology was between 58% and 100%. At present, the prevalence, characteristics and drug resistance of Escherichia coli are the focus of global attention. The characteristics of drug resistance and integron of Escherichia coli in rabbit farms are analyzed in this experiment. It has a certain understanding of the drug resistance of Escherichia coli from rabbits in Tai'an area, which provides a preliminary basis for clinical rational selection of antibiotics, and can prevent and treat the infection caused by Escherichia coli more comprehensively in clinical work. In order to make more targeted preventive measures.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.291
本文編號(hào)：2146650
[Abstract]:Escherichia coli exists widely in human and animal body. It is a resident bacteria in intestinal tract and is also a kind of conditional pathogenic bacteria. Some special serotypes of Escherichia coli have pathogenicity to human, especially to infants and animals. Rabbit colibacillosis is a fulminant intestinal infection caused by pathogenic Escherichia coli and its toxin, which has the characteristics of short incubation period and high mortality. Rabbits of all ages are susceptible to Escherichia coli. The purpose of this study was to understand the prevalence and drug resistance of Escherichia coli in rabbit farms in Tai'an area. In March 2016, 20 rabbit feces were collected from three rabbit farms in Taian (Ningyang, Xintai and Dongping), and 60 samples were collected from three farms. The collected samples were separated by Gram staining and 16srRNA. The biochemical characteristics of Escherichia coli were observed by biochemical test. After isolation and identification of Escherichia coli, the susceptibility of Escherichia coli isolates to 14 kinds of antibiotics was detected by disk Agar diffusion method (K-B), which indicated the resistance of Escherichia coli strains isolated from rabbits, and the resistance phenotype of Escherichia coli was also known. After that, the presence of 24 resistant genes in Escherichia coli strains isolated from rabbits was detected. Then we tested the Escherichia coli isolates by detecting the class I integron of bacteria to understand the drug resistance of the bacteria. Finally, the molecular diversity of Escherichia coli isolated from rabbit was analyzed by repeated common sequence (ERIC) PCR of Enterobacter coli. After isolation and identification of Escherichia coli, 55 strains of non-repeated Escherichia coli were isolated. The results of drug sensitivity test showed that the 55 strains of Escherichia coli showed different degrees of resistance to 14 kinds of antibiotics used in the experiment, and the sensitivity was different. Tetracycline was insensitive to tetracycline, and the drug resistance rate was 78.2, but it was sensitive to ceftazidime, ceftriaxone, imipenem and amikacin, and the resistance rate was low. The results of drug resistance gene detection showed that 11 resistant genes were detected in 55 strains of Escherichia coli isolated from rabbits, among which the highest detection rate was detected by 尾 lactamases blaTEM and blaCTX-M genes. The detection rates of blaTEM (98.18%) and blaCTX-M (94.5%), followed by sulfonamides resistant gene sul-2 (58.18%). The detection of class I integron showed that 17 strains of Escherichia coli carrying class I integron were detected in 55 strains of Escherichia coli isolated from rabbits, and the detection rate was 30.91%. In this study, we found that there were 7 drug resistant gene cassettes in the class I integron, which were dfrA17 aadA5, dfrA1 catB3 aac A4A4, LinaadA2 lindfraadA1, aadA22dfrA12 orfF addA2aadA16 dfrA27 arr-3.ERIC-PCR. The isolates were divided into 23 genotypes (A-W), and their homology was between 58% and 100%. At present, the prevalence, characteristics and drug resistance of Escherichia coli are the focus of global attention. The characteristics of drug resistance and integron of Escherichia coli in rabbit farms are analyzed in this experiment. It has a certain understanding of the drug resistance of Escherichia coli from rabbits in Tai'an area, which provides a preliminary basis for clinical rational selection of antibiotics, and can prevent and treat the infection caused by Escherichia coli more comprehensively in clinical work. In order to make more targeted preventive measures.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.291
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