發(fā)布時間：2018-07-17 07:32

【摘要】：蘇尼特羊?qū)倜晒叛蚱贩N,具有耐寒、易放牧和肉質(zhì)鮮美等優(yōu)良特性,其不足之處是生產(chǎn)性能較低。本研究以300只蘇尼特羊(其中產(chǎn)單羔188只,產(chǎn)雙羔112只)為研究對象,同時選取小尾寒羊和湖羊各50只作為對照,整個研究群體總數(shù)為400只。利用聚合酶鏈反應(yīng)限制性片段長度多態(tài)性PCR-RFLP結(jié)合DNA測序技術(shù),分析BMPR-IB、BMP15和GDF9三個基因中多個不同突變位點對蘇尼特羊多胎性狀的影響,旨在為提高蘇尼特羊的繁殖力提供理論支持。主要研究結(jié)果有:(1)在部分產(chǎn)雙羔的蘇尼特羊群中發(fā)現(xiàn)FecB突變(A746G),突變類型為雜合型(AG)和純合突變型(GG),基因型頻率分別為0.089和0.009,A和G等位基因頻率為0.946和0.054;產(chǎn)雙羔蘇尼特羊群體在該位點PIC=0.097,表現(xiàn)為低度多態(tài);產(chǎn)單羔蘇尼特羊群體在該位點無多態(tài)性;產(chǎn)單羔蘇尼特羊群體中G等位基因頻率顯著低于產(chǎn)雙羔蘇尼特羊群體中G等位基因頻率(P0.01);關(guān)聯(lián)性分析表明,FecB位點與蘇尼特羊的雙羔率有關(guān)(P0.001)。(2)所選蘇尼特羊群體在BMP15基因的FecXB、FecXL、FecXH、FecXG和FecXI五個位點中均未發(fā)現(xiàn)突變,全部為純合野生型。(3)所選蘇尼特羊群體在GDF9基因上的FecGH FecGF、FecGE和G4四個位點上均無多態(tài)性,全部為純合野生型。(4)通過測序分析首次發(fā)現(xiàn)部分蘇尼特羊BMP15基因的內(nèi)含子發(fā)生以下突變:A115G、G117A、C342A、G419A、A1168G 以及 G2129T。本研究結(jié)果證明,FecB突變位點可作為分子標記用于提高蘇尼特羊產(chǎn)羔率,然而蘇尼特羊雙羔性狀的分子遺傳機制仍有待進一步深入研究。
[Abstract]:Sunita sheep is a Mongolian sheep breed, which has the advantages of cold tolerance, easy grazing and delicious meat quality, but its production performance is low. In this study, 300 Sunita sheep (including 188 single lambs and 112 double lambs) were selected as control, and 50 small tail Han sheep and 50 Hu sheep were selected as control, the total number of the whole research population was 400. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technique were used to analyze the effects of different mutation sites of BMPR-IBBMP15 and GDF9 genes on the fertility traits of Sunita sheep. The aim is to provide theoretical support for improving the fecundity of Sunita sheep. The main results are as follows: (1) FecB mutation (A746G) was found in some Sunita sheep with two lambs, the mutation types were heterozygous (AG) and homozygous (GG), the genotype frequencies were 0.089 and 0.009A and G allele frequencies were 0.946 and 0.054, respectively; The population of Niett sheep showed low polymorphism at PIC-0.097 at this locus. There was no polymorphism at this locus in single lamb Sunita sheep population. The frequency of G allele in single lamb Sunita sheep was significantly lower than that in double lamb Sunita sheep (P0.01), and the correlation analysis showed that the frequency of G allele in Sunita sheep was related to the rate of double lambs (P0. 001). (2). No mutations were found at the five FecXHG and FecXI loci of the BMP15 gene. All were homozygous wild type. (3) there was no polymorphism at the four loci of FecGH FecGFGE and G4 on the GDF9 gene in the selected Sunita sheep population. All of them were homozygous wild type. (4) the following mutations were found in the intron of part of Sunita sheep BMP15 gene by sequencing. The following mutations occurred in the intron of the BMP15 gene: 10 A115G, G117A, C342A, G419A, A1168G and G2129T. The results show that the FecB mutation site can be used as a molecular marker to improve the lambing rate of Sunita sheep. However, the molecular genetic mechanism of double lamb traits of Sunita sheep needs to be further studied.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S826

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