本文選題：三疣梭子蟹 + 運(yùn)輸方法�。� 參考：《中國海洋大學(xué)》2015年碩士論文

【摘要】：1不同運(yùn)輸方法對三疣梭子蟹存活和生理反應(yīng)的影響研究了不同運(yùn)輸方法(低溫水運(yùn)、低溫直接干露和低溫間接干露)下運(yùn)輸3h后,三疣梭子蟹的心率、血清皮質(zhì)醇、葡萄糖和乳酸含量變化,探討運(yùn)輸脅迫后梭子蟹的存活和生理反應(yīng)。結(jié)果顯示： (1)3h運(yùn)輸結(jié)束后,低溫間接組中幼蟹成活率僅為85%,低于其它2組；相同運(yùn)輸方式下,成蟹的成活率大于幼蟹。(2)3h運(yùn)輸結(jié)束后,三種運(yùn)輸方式中梭子蟹血清皮質(zhì)醇、葡萄糖和乳酸含量均有相似的變化趨勢。干運(yùn)組中這三種物質(zhì)的含量顯著高于對照組和水運(yùn)組(P0.05)；而水運(yùn)組與對照組差異不顯著(P0.05)。 (3)干露0.5h時,梭子蟹的心率達(dá)到最低值17次/min；干露3h時,梭子蟹的心牢上升到22次/min；3-4h干露期間一直維持在22次/min。研究結(jié)果表明,3h可能是梭子蟹的臨界干露時長；幼蟹運(yùn)輸應(yīng)采用低溫水運(yùn)法,短距離運(yùn)輸(小于3h運(yùn)輸時長)成蟹時可選擇低溫干法運(yùn)輸方式。2干露對三疣梭子蟹抗氧化和應(yīng)激能力的影響在實(shí)驗(yàn)室條件下,研究了干露時間(0.5、1.5和3h)脅迫對三疣梭子蟹(Portunus trituberculatus)抗氧化能力和應(yīng)激能力的影響,初步探討了能夠較靈敏地指示梭子蟹健康狀況的脅迫指標(biāo)。結(jié)果顯示： (1)干露時間脅迫對超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均無顯著影響(P0.05)；但干露時間顯著影響肝胰臟總抗氧化能力(T-AOC)水平、熱休克蛋白70(Hsp70)水平和肌肉乳酸含量(P0.05)。 (2)0.5h干露脅迫后,SOD活力在入水恢復(fù)4h時達(dá)到峰值；MDA含量在入水恢復(fù)2-10h內(nèi)顯著高于對照組水平(P0.05)：T-AOC水平在恢復(fù)階段基本維持在對照組水平。1.5h和3h干露后,SOD活力在入水恢復(fù)階段一直維持在對照組水平,且各時間點(diǎn)與對照組差異不顯著(P0.05)。1.5h干露后MDA含量在入水恢復(fù)階段呈先升高后降低趨勢,各時間點(diǎn)與對照組差異不顯著(P0.05)；而3h干露后入水恢復(fù)10h時顯著增加(P0.05)。1.5h干露后入水恢復(fù)4h時,T-AOC水平顯著高于對照組(P0.05)：3h干露后入水恢復(fù)0.5h時顯著升高(P0.05),2-10h內(nèi)基本恢復(fù)到對照組水平。干露時間脅迫后,乳酸含量均在入水恢復(fù)0.5h時達(dá)到最大值；Hsp70水平均在在入水恢復(fù)2-4h內(nèi)達(dá)到峰值。研究結(jié)果表明,相比于其它所選指標(biāo),T-AOC水平能夠較敏感地反映干露時間脅迫對機(jī)體的生理狀態(tài)的影響；SOD和MDA相互配合能夠較靈敏地反映不同干露時長后機(jī)體在恢復(fù)階段的免疫水平。3三疣梭子蟹對不同溫度下的干露脅迫的生理響應(yīng)在實(shí)驗(yàn)室條件下,研究了不同溫度(16℃和31℃)下的干露脅迫及脅迫后入水恢復(fù)時間對三疣梭子蟹肝胰腺中SOD、MDA、T-AOC、Hsp70水平及肌肉中乳酸含量的影響,干露時長為3h。研究結(jié)果顯示：(1)干露溫度脅迫對MDA及乳酸含量均無顯著影響(P0.05),但顯著影響SOD、T-AOC和Hsp70水平(P0.05)。(2)16-C下干露脅迫3h后,SOD活力在入水恢復(fù)4h時達(dá)到峰值,但各時間點(diǎn)與對照組均未達(dá)到顯著差異(P0.05)。入水恢復(fù)4h時,MDA含量顯著升高(P0.05)；10h時,基本恢復(fù)到對照組水平。入水恢復(fù)0.5-4h內(nèi),Hsp70表達(dá)量顯著低于對照組(P0.05)；10h時,Hsp70表達(dá)量顯著高于對照組水平(P0.05)。 (3)31℃下干露脅迫3h后,SOD活力在入水恢復(fù)0.5-2h內(nèi)顯著降低(P0.05)；4h時,基本恢復(fù)到對照組水平。入水恢復(fù)0-4h內(nèi),MDA含量基本維持在對照組水平；10h時,MDA含量達(dá)到峰值,與對照組差異顯著(P0.05)。在入水恢復(fù)0.5h時,Hsp70表達(dá)量達(dá)到峰值,顯著高于對照組(P0.05)；2-10h內(nèi),基本維持在對照組水平。 (4)不同溫度下的干露脅迫后,T-AOC水平在入水恢復(fù)各時間點(diǎn)均顯著高于對照組(P0.05)：乳酸含量在入水恢復(fù)2h時達(dá)到峰值,與對照組差異顯著(P0.05)。研究結(jié)果表明,低溫(16℃)下的干露脅迫有利于梭子蟹保持良好的生理狀態(tài),故在干法運(yùn)輸時,應(yīng)該適當(dāng)?shù)亟档蜏囟取?br/>[Abstract]:1 the effects of different transportation methods on the survival and physiological response of Portunus three were studied. The changes of heart rate, serum cortisol, glucose and lactic acid content of the three Portunus warts after transportation of 3H under different transportation methods (low temperature water transport, direct dry dew and indirect dry dew of low temperature) were studied. The survival and physiological responses of the shuttle crab after transportation stress were discussed. (1) (1) after the end of the transport, the survival rate of young crabs in the low temperature indirect group was only 85%, which was lower than the other 2 groups; the survival rate of the crab was greater than that of the young crab. (2) after the end of the transport, the content of serum cortisol, glucose and lactic acid in the three transport modes of the three types of transportation were similar. The content of the three substances in the dry Transport Group The difference between the control group and the control group (P0.05) was significantly higher than that of the control group (P0.05). (3) when the dry dew 0.5h, the heart rate of the shuttle crab reached the lowest value of 17 times /min; when the dry dew 3h, the heart of the shuttle crab rose to 22 times /min, and the 3-4h dry dew kept in the 22 /min. research results during the 3-4h dry dew show that 3H may be the critical dry dew of the shuttle crab. Long time, young crab transportation should adopt low temperature water transport method, short distance transport (less than 3H transportation time), the effect of.2 dry dew on the antioxidant and stress ability of three wart crabs can be selected. Under the laboratory conditions, the stress of dry dew (0.5,1.5 and 3H) stress on the oxygen resistance of three verruca crabs (Portunus trituberculatus) is studied. The results showed that (1) dry exposure time stress had no significant effect on the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) (P0.05), but the drying time significantly affected the total antioxidant capacity (T-AOC) of hepatopancreas (T-AOC). Level, heat shock protein 70 (Hsp70) level and muscle lactic acid content (P0.05). (2) after 0.5h dry exposure stress, SOD activity reached its peak value when water was restored to 4h, and MDA content was significantly higher than that of control group (P0.05):T-AOC level in the recovery phase (P0.05) in the recovery phase (P0.05) at the level of.1.5h and 3H dry dew in the control group, and SOD vigor was entered into water restorer. There was no significant difference between the control group and the control group at the complex stage, and there was no significant difference between the control group and the control group at each time point (P0.05).1.5h dry dew. The MDA content increased first and then decreased, and the difference between the time points and the control group was not significant (P0.05), while the 3H dry dew after the water resumed 10h significantly increased (P0.05).1.5h dry dew and recovered 4h, T-AOC. The level was significantly higher than that of the control group (P0.05):3h dry dew after the recovery of 0.5h (P0.05), and the 2-10h content was basically restored to the control level. After the exposure time, the lactic acid content reached the maximum when the water was restored to 0.5h, and the Hsp70 level was reached to the peak value in the return of water to 2-4h. The results showed that compared with the other selected fingers, the results showed that The T-AOC level can sensitively reflect the effect of dry exposure time stress on the physiological state of the body, and the interaction between SOD and MDA can sensitively reflect the immune level of the body in the recovery phase of the different dry dew time and.3 three of the shuttle crab under different temperatures under the laboratory conditions. The effect of dry dew stress at temperature (16 and 31 C) and water recovery time after stress on SOD, MDA, T-AOC, Hsp70 level and lactic acid content in muscle and pancreas in the hepatopancreas of three verruca crabs. The results of 3h. study showed that (1) dry dew temperature stress had no significant effect on MDA and lactic acid content (P0.05), but significantly affected SOD, T-AOC and Hsp70. Level (P0.05). (2) after the exposure to 3H under 16-C, the activity of SOD reached its peak value when the water was restored to 4h, but all time points were not significantly different from that of the control group (P0.05). When the water was restored to 4h, the content of MDA increased significantly (P0.05); 10h, basically recovered to the level of the control group. The expression of Hsp70 was significantly lower than that of the control group; Hsp70 expression was significantly lower than that of the control group; while the 10h, the Hsp70 expression was significantly lower than that of the control group. At h, the expression of Hsp70 was significantly higher than that of the control group (P0.05). (3) after the exposure to 3H at 31 C, the activity of SOD decreased significantly (P0.05) in the return of water to 0.5-2h. When 4h, the level of the control group was basically restored to the control group. The MDA content was basically maintained at the level of the control group; 10h, the MDA content reached the peak, and was significantly different from the control group. When the water was restored to 0.5h, the expression of Hsp70 reached a peak value, which was significantly higher than that of the control group (P0.05); in 2-10h, the level of the control group was basically maintained. (4) after the dry dew stress at different temperatures, the T-AOC level was significantly higher than the control group (P0.05) at all time points in the water recovery. The content of lactic acid reached the peak when the water was restored to 2h, and the difference was significant between the control group and the control group. (P0.05). The results show that the dry dew stress at low temperature (16 C) is beneficial to the good physiological state of the shuttle crab, so the temperature should be reduced properly during the dry transportation.
【學(xué)位授予單位】：中國海洋大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S917.4

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