本文選題：配方施肥 + 干旱脅迫　； 參考：《中國林業(yè)科學研究院》2017年碩士論文

【摘要】：本試驗通過設(shè)置N、P、K肥3因子、3水平涉及9個處理辣木幼苗配方施肥試驗,研究其不同施肥配方處理下的苗木的生長效應(yīng)和產(chǎn)量構(gòu)建的變化,確定適合辣木有效生長和高產(chǎn)的肥料配方。通過研究4種源辣木幼苗對干旱脅迫的生長、生物量和生理生化響應(yīng),觀測苗木的生長狀況,測定生長性狀、各部分生物量、葉綠素含量、超氧化物歧化酶(SOD)活性、過氧化物酶(POD)活性、過氧化氫酶(CAT)活性、丙二醛(MDA)含量、可溶性蛋白(PS)含量、脯氨酸(Pro)含量等指標,探討不同種源辣木幼苗的耐旱性差異,篩選出耐旱的優(yōu)良種源。主要研究結(jié)果如下:(1)施肥處理對各時期生長有顯著的促進作用,營養(yǎng)生長期N肥和K肥占主導(dǎo)作用,適合株高和地徑生長的最優(yōu)組合為N_(150)P_(40)K_(100),適合冠幅和分枝數(shù)生長的最優(yōu)組合為N_(85)P_(85)K_(100)。不同施肥水平對生殖生長有明顯促進作用,均在處理N_(150)P_(40)K_(100)中達最大值,且適合辣木產(chǎn)量增加的最優(yōu)組合為N_(40)P_(150)K_(100)。(2)隨著干旱脅迫的加劇,辣木株高、地徑、生物量總體均呈下降的趨勢,總體而言,輕度脅迫和中度脅迫時下降較快,且不同種源降幅不同,其中MD種源和KNY種源降幅較低。(3)干旱脅迫對不同種源光合色素影響有所差異,其中YD種源和KNY種源含量較少,降幅也低,PK種源含量較高,但變化差異較大。總體而言,4種源辣木的Chla、Chl(a+b)、Car含量變化趨勢表現(xiàn)為隨脅迫程度的加深呈現(xiàn)出先增加后下降的趨勢,重度脅迫含量最低。(4)不同種源生化指標在相同處理下差異不明顯,但干旱脅迫對辣木的生化指標影響較大,SOD和CAT均隨脅迫程度的加深表現(xiàn)出先增加后下降的趨勢,輕度干旱脅迫下增加趨勢不明顯,重度脅迫迅速下降,下降到一定程度后,趨于穩(wěn)定。而POD、MDA、Pro表現(xiàn)為隨脅迫加劇而遞增,但脅迫后期增速較緩。GS總體表現(xiàn)為下降的趨勢,種源間差異較小,但在中度脅迫時降幅較大,差異顯著。(5)POD和CAT之間呈極顯著正相關(guān)(P0.01),POD和SP之間呈極顯著負相關(guān)(P0.01),SP與MDA呈極顯著負相關(guān)(P0.01),SP與Pro呈顯著負相關(guān)(P0.05)。(6)采用隸屬函數(shù)法對4種源辣木的耐旱性作綜合評價,不同種源辣木的耐旱性存在明顯的差異,耐旱性由強到弱為:MD種源㧐KNY種源㧐YD種源㧐PK種源。
[Abstract]:In this experiment, the effect of growth and the change of yield construction of the seedlings under different fertilizer treatments were studied by setting up the level of 3 factors and 3 factors of N ~ (2 +) P ~ (+) K fertilizer, which involved nine treatments of hot wood seedling formula fertilization, in order to study the effect of fertilizer application on the growth of seedlings. The fertilizer formula suitable for effective growth and high yield of spicy wood was determined. The growth, biomass, physiological and biochemical responses to drought stress were studied, and the growth characteristics, biomass, chlorophyll content and activity of superoxide dismutase (SOD) were measured. The activities of peroxidase POD, catalase (CAT), malondialdehyde (MDA), soluble protein (PSN), proline (Proprol) and so on were studied. The main results were as follows: (1) Fertilizer treatment had a significant promoting effect on the growth of each stage, N fertilizer and K fertilizer played the leading role in the vegetative growth period, and the optimum combination for plant height and ground diameter growth was N ~ (150) P ~ (+) ~ (40) K ~ (+) ~ (1) C ~ (-1), and the optimum combination for the growth of crown and branch number was N ~ (85) P ~ (+) ~ + ~ (85) K ~ (+) ~ (100) ~ (-1) for the growth of plant height and ground diameter. Different fertilization levels had a significant effect on reproductive growth, all of them reached the maximum value in the treatment of NSCL 150 PX 40 KT / L), and the optimum combination suitable for the increase of spicy wood yield was NSCT 40 PSP 150 KT. 2) with the increase of drought stress, the plant height, ground diameter and biomass of Agaricum officinalis were all decreasing, as a whole, the plant height, ground diameter and biomass showed a downward trend, as a result of drought stress, the plant height, ground diameter and biomass were decreased. In general, the decrease of light stress and moderate stress was faster, and the decrease was different among different provenances, among which MD provenance and KNY provenance had a lower decrease.) drought stress had different effects on photosynthetic pigments of different provenances. Among them, the content of YD provenance and KNY provenance was less, and the decrease was lower. The content of PK provenance was higher, but the variation was significant. As a whole, the change trend of Chlaanus chllraebtii Car content showed that the content increased first and then decreased with the deepening of stress degree, and the lowest content of severe stress was 0.14) the biochemical indexes of different provenances had no significant difference under the same treatment. But the effect of drought stress on the biochemical indexes was greater than that under drought stress. With the deepening of the stress degree, both SOD and CAT showed a tendency of increasing first and then decreasing, but the increasing trend was not obvious under mild drought stress, but decreased rapidly under severe stress, and then decreased to a certain degree. Tend to stabilize. However, the growth rate of PODN MDAP increased with the increase of stress, but in the late stage of stress, the growth rate was slow. GS showed a downward trend, and the difference between provenances was small, but the decrease was larger in moderate stress. There was a very significant positive correlation between P0.01POD and SP. There was a very significant negative correlation between P0.01P and MDA. There was a significant negative correlation between P0.01P and Pro (P0.05P0.05P0.05N. Y6) the drought tolerance of four provenances was evaluated by membership function method, and the relationship between P0.01P and MDA was evaluated by the membership function method, and the drought resistance of four provenances was evaluated by using the membership function method, and the results showed that the drought tolerance of the four provenances was evaluated by using the membership function method. There were significant differences in drought tolerance between different provenances, and the drought tolerance from strong to weak was the provenance of KNY provenance and the PK provenance of YD provenance.
【學位授予單位】：中國林業(yè)科學研究院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S792.99

【參考文獻】

相關(guān)期刊論文 前10條

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本文編號：1990757