本文選題：抗生素 + 微生態(tài)制劑　； 參考：《河南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本文旨在基礎(chǔ)日糧中加入不同比例的濕態(tài)發(fā)酵全價(jià)料,探討其對斷奶仔豬的生產(chǎn)性能、粗蛋白消化率、血清生化指標(biāo)、小腸黏膜結(jié)構(gòu)和二糖酶活、腸道菌群的影響。試驗(yàn)選取體況良好、體重為(8.20±0.16)kg的杜×長×大型斷奶仔豬160頭,隨機(jī)分配于以下5種日糧(每組4個重復(fù),每個重復(fù)8頭豬)中:1)基礎(chǔ)日糧+20mg硫酸粘桿菌素+12mg恩拉霉素(抗生素組);2)基礎(chǔ)日糧+0.1%枯草與地衣芽孢桿菌的復(fù)合型微生態(tài)制劑(微生態(tài)制劑組);3)10%濕態(tài)發(fā)酵全價(jià)料替代部分無抗基礎(chǔ)日糧(10%發(fā)酵料組);4)20%濕態(tài)發(fā)酵全價(jià)料替代部分無抗基礎(chǔ)日糧(20%發(fā)酵料組);5)30%濕態(tài)發(fā)酵全價(jià)料替代部分無抗基礎(chǔ)日糧(30%發(fā)酵料組)。試驗(yàn)分成2個部分,結(jié)果如下:試驗(yàn)一:(1)與抗生素組和微生態(tài)制劑組相比,20%、30%發(fā)酵料組顯著(P0.05)提高了仔豬的日增重;與抗生素組相比,發(fā)酵料組均顯著(P0.05)降低了腹瀉率,對料重比有一定的降低(P0.05);各處理組在仔豬的日采食量方面無顯著差異(P0.05)。(2)日糧中添加適量的發(fā)酵料可在一定程度上提高仔豬的粗蛋白消化率(P0.05)。(3)與抗生素組相比,10%、30%發(fā)酵飼料組可顯著(P0.05)提高仔豬血清的球蛋白水平;30%發(fā)酵飼料組顯著(P0.05)提高了IgM含量,而發(fā)酵料組均對其它血清指標(biāo)無顯著影響(P0.05),但能在一定程度上提高仔豬血清中總蛋白水平和免疫球蛋白含量。與微生態(tài)制劑組相比,20%發(fā)酵飼料組顯著(P0.05)提高了仔豬的血糖含量;發(fā)酵飼料組均使血清尿素氮水平下降(P0.05),但其血清酶(谷丙/草轉(zhuǎn)氨酶)活、甘油三酯和相應(yīng)膽固醇的濃度無顯著差異(P0.05)。試驗(yàn)二:(1)20%、30%無抗發(fā)酵全價(jià)料組較抗生素組和微生態(tài)制劑組提高(P0.05)了仔豬糞樣中乳酸菌數(shù),且發(fā)酵料組均減少(P0.05)了大腸桿菌數(shù),對酵母菌數(shù)有一定的提高(P0.05)。(2)與抗生素組相比,發(fā)酵料組均增加(P0.05)了仔豬的空腸VH、VH/CD值,降低了十二指腸CD;且10%、20%發(fā)酵全價(jià)料組增加(P0.05)了仔豬十二指腸VH、VH/CD;20%、30%發(fā)酵飼料組能顯著(P0.05)增加仔豬回腸VH、VH/CD和降低回腸CD;20%發(fā)酵飼料組顯著(P0.05)增加了十二指腸黏膜上皮內(nèi)淋巴細(xì)胞數(shù)。(3)與抗生素組相比,發(fā)酵料組均顯著(P0.05)提高了仔豬空腸蔗糖酶活,且20%、30%發(fā)酵料組顯著(P0.05)提高了十二指腸麥芽糖酶活,30%發(fā)酵料組顯著(P0.05)提高了其空腸乳糖酶活。(4)微生態(tài)制劑組和10%發(fā)酵料組對仔豬直腸菌群的相似性影響較大,而20%、30%發(fā)酵料組則對其結(jié)腸影響較大;除20%發(fā)酵料組外各處理組結(jié)腸菌群的多樣性指數(shù)均高于直腸,且抗生素組均低于發(fā)酵料組;抗生素組和微生態(tài)制劑組仔豬結(jié)腸菌群的均衡度和豐富度相對高于直腸,且抗生素組的均衡度和豐富度均低于發(fā)酵料組。結(jié)果表明,在日糧中添加適量的濕態(tài)發(fā)酵全價(jià)飼料在一定程度上可提高斷奶仔豬的生產(chǎn)性能,修復(fù)受損的腸道黏膜,提高二糖酶活,并改善菌群的平衡狀態(tài),提高斷奶仔豬的抗病力和抗應(yīng)激能力。
[Abstract]:The purpose of this study was to investigate the effects of different proportion of wet fermenting total feed on weaning piglets' production performance, crude protein digestibility, serum biochemical index, intestinal mucosal structure, disulase activity and intestinal flora.One hundred and sixty piglets with a body weight of 8.20 鹵0.16)kg were randomly assigned to the following 5 diets (4 repeats in each group).20mg (antibiotic group 2) A compound microecological preparation of 0.1% subtilis and Bacillus licheniformis (microecological preparation group)Substitution of 10% fermentable diets with 10% non-resistant basal diets for 30% wet fermenting materials in 30% fermentable diets with 30% Wet fermenting materials for 30% fermentable diets without resistance to the basic diets of 20% non-resistant basal diets, and replacing 30% fermentable diets with 30% fermenting materials for 30% fermentable diets without resistance.The experiment was divided into two parts. The results were as follows: experiment 1: 1) compared with the antibiotic group and the microecological preparation group, the weight gain of piglets increased significantly (P0.05), and compared with the antibiotic group, the diarrhea rate decreased significantly in the ferment group (P 0.05).There was no significant difference in the daily feed intake of piglets among the treatments (P0.05. 0. 5%, P 0. 05. 0. 0. 2) the addition of appropriate amount of fermenting material to the diet could to some extent improve the crude protein digestibility of piglets (P0. 05%. P0. 05%)) compared with the antibiotic group, 1030% of the treatments had higher crude protein digestibility than that of the antibiotic group.The serum globulin level of piglets was significantly increased in fermenting feed group (P0.05), and the IgM content was increased in fermenting feed group (P0.05).However, the fermenting material group had no significant effect on other serum indexes, but it could improve the serum total protein level and immunoglobulin content in piglets to some extent.Compared with the microecological preparation group, the 20% fermenting feed group significantly increased the blood sugar content of the piglets, and the serum urea nitrogen level decreased P0.05%, but the activity of serum glutamic acid / oxaloacetic transaminase (glutamic acid / oxaloacetic transaminase) was decreased in the fermenting feed group.There was no significant difference between the concentration of triglyceride and cholesterol.In experiment 2, the number of lactic acid bacteria in fecal samples of piglets was increased in the 30% non-antiferment group (30%) compared with the antibiotic group and the microecological preparation group (P 0.05), and the number of Escherichia coli was decreased in the fermenting feed group, and the number of yeast was increased to a certain extent, compared with the antibiotic group.The VHH / CD / CD value of jejunum of piglets was increased in fermentative feed group (P 0.05), and the VHH / CD ratio in jejunum of piglets was increased.Decreased duodenal CDI, and increased P0.05 in 10% 20% fermenting whole feed group (P 0.05) significantly increased VHH / CD20% fermenting feed group (P0.05) increased VHN VHH / VHP / CD ratio and decreased ileum CD20% fermentative feed group (P0.05) increased duodenal mucosal epithelium Lymphoma significantly in piglets fed with VHH / CD20% fermentable feed group (P < 0.05).Compared with antibiotic group,The sucrase activity in jejunum of piglets was increased significantly in fermenting material group (P0.05).And 20% 30% fermentation material group significantly increased the duodenal maltase activity and 30% fermentation material group significantly increased the jejunum lactase activity. 4) the microecological preparation group and the 10% fermentation feed group had great influence on the similarity of rectal flora of piglets.The colonic diversity index of each treatment group except 20% fermenting material group was higher than that of rectum, and the antibiotic group was lower than that of fermenting material group.The equilibrium and richness of colonic flora of piglets in antibiotic group and microecological preparation group were higher than those in rectum, and the equilibrium and richness of antibiotic group were lower than those of fermenting material group.The results showed that the production performance of weaning piglets was improved, the damaged intestinal mucosa was repaired, the activity of disulase was improved, and the balance of bacterial flora was improved.To improve the ability of anti-disease and anti-stress of weaning piglets.
【學(xué)位授予單位】：河南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S828.5

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本文編號：1771950