本文選題：彩色馬蹄蓮　切入點：花青素苷　出處：《昆明理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：彩色馬蹄蓮(Zantedeschi hybridSpr)天南星科馬蹄蓮屬原產(chǎn)地非洲,由于其奇特的花型、豐富艷麗的色彩在世界花卉市場廣受歡迎,是切花,盆栽的佳品,被譽為21世紀花卉之星�，F(xiàn)今用于觀賞的馬蹄蓮多為雜交品種�；ㄉ怯^賞植物最為主要的觀賞性狀,直接影響這花卉的市場價值。本研究以27個彩色馬蹄蓮為材料,運用測色法、組織切片法、色素成分及色素含量分析等方法對彩色馬蹄蓮佛焰苞色的形成進行研究。經(jīng)分析研究得出以下結(jié)論:(1)使用色差儀對收集到的27個彩色馬蹄蓮品種進行花色評價,使用聚類分析并結(jié)合色空間(CIELab Space)綜合分析將彩色馬蹄蓮分成黑色系(Black group)、紫紅色系(Amaranth group)、紅色系(Redgroup)、黃色系(Yellow group)、粉白色系(Pink group)、橙色系(Orange group)六大色系,為彩色馬蹄蓮色系分類與定義提供理論依據(jù)。(2)本研究通過徒手制作植物組織切片,對彩色馬蹄蓮佛焰苞組織結(jié)構(gòu)進行顯微觀察,分析彩色馬蹄橫切面解剖結(jié)構(gòu)與呈色之間的關(guān)系。結(jié)果發(fā)現(xiàn)彩色馬蹄蓮佛焰苞呈扁平狀磚形,并且色素細胞層數(shù)越多,佛焰苞的顏色越深。紅色系與橙色系品種,含有花青素苷與黃酮、黃酮醇,花青素苷存在于上下表皮細胞,黃酮、黃酮醇在中部葉肉組織內(nèi)富集,兩種色素復(fù)合產(chǎn)生了不同程度的紅色、橙色。(3)本研究通過利用高效液相色譜-光電二極管陣列檢測(HPLC-DAD)和高效液相色譜-電噴霧離子化-質(zhì)譜聯(lián)用(HPLC-ESI-MS)技術(shù),并采用標準品半定量的方法,對所收集到彩色馬蹄蓮佛焰苞中主要的色素成分及含量進行定量與定性分析。分析彩色馬蹄蓮黑色品種花青素苷生物合成途徑,外部環(huán)境對花青素苷積累的影響,色彩表型L*a*b*值與色素成分含量關(guān)系顯著,I*值與總的花青素苷的含量呈負相關(guān),隨花青素苷含量的升高,亮度降低。運用紫外分光法對收集到的佛焰苞中的胡蘿卜素進行測量(UV-visiblespectra),結(jié)果表明,所收集到的彩色馬蹄蓮品種中均未檢測出胡蘿卜素成分,因此可以認定彩色馬蹄蓮黃色系、橙色系品種,使其呈黃色和橙色的主要色素物質(zhì)為黃酮、黃酮醇,而不是類胡蘿卜素,并且黃度(b*)與黃酮、黃酮醇(TF)的含量呈正相關(guān)的關(guān)系。(4)利用illumnina/Solexa第二代高通量轉(zhuǎn)錄組測序技術(shù),對彩色馬蹄蓮小黑妞、浪漫、范圖拉這三個品種進行轉(zhuǎn)錄組測序,共獲得446340條高質(zhì)量轉(zhuǎn)錄組短數(shù)列,平均長度為785bp。在Nr數(shù)據(jù)庫中有注釋的共有29816條Unigene,將所得到的相關(guān)映射基因映射到KEGG數(shù)據(jù)庫提供的參考目錄中,其中55條序列可能設(shè)計彩色馬蹄蓮花色形成:并結(jié)合代謝信息與代謝物質(zhì)變化研究,闡述彩色馬蹄蓮花青素苷代謝途徑。
[Abstract]:Calla Lily (Zantedeschi hybridSpr) of Araceae Zantedeschia origin in Africa, because of its peculiar flowers, rich bright colors are popular in the world, the flower market is cut flowers, potted flowers Jiapin, known as the twenty-first Century star. Now mostly for ornamental Calla cultivars. Ornamental flower color is the most as the main ornamental characters, which directly affects the flower market value. In this study, 27 Zantedeschia as materials, using colorimetry, histological method, pigment composition and pigment content analysis method of Zantedeschia hasuhotoke spathe color formation were studied. After analysis the following conclusions: (1) the evaluation of the collected color using color difference meter 27 Zantedeschia cultivars, using cluster analysis and combined with the color space (CIELab Space) will be a comprehensive analysis of Zantedeschia into black (Black group), purple red The Department (Amaranth group), red (Redgroup), yellow (Yellow group), white powder (Pink group), orange (Orange group) six colors, and provide a theoretical basis for the classification of Zantedeschia color and definition. (2) this study by hand making plant tissue sections of microscopic observation of Zantedeschia hasuhotoke spathe structure, analysis of anatomical structure and relationship between the cross section of a color. The Calla Zantedeschia hasuhotoke spathe was flat brick, and more layers of pigment cells, spathe color deeper. Red and orange varieties contain anthocyanins and flavonoids., flavonols, anthocyanins present in the upper and lower epidermal cells, flavone, flavonol accumulation in the middle mesophyll tissue, two kinds of orange pigment composite had a different degree of red. (3) this study by using high performance liquid chromatography with photodiode array detection (HPLC -DAD) and high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) technology, and using the standard semi quantitative method, collected pigment composition and content of the main color of horseshoe hasuhotoke spathe in quantitative and qualitative analysis. The analysis of Zantedeschia black species anthocyanin biosynthetic pathway. The influence of external environment on anthocyanin accumulation, the relationship between color value and the phenotype of L*a*b* pigment composition content significantly, I* was negatively correlated with the total anthocyanin content, anthocyanin content increased with brightness, reduced. Using ultraviolet spectrophotometry to collect spathaceous in carotenoids were measured (UV-visiblespectra), the results show that the collected Zantedeschia cultivars were not detected in carotenoid composition, it can be found Zantedeschia yellow, orange varieties, the main pigment yellow and orange The material for flavonoids, flavonols, instead of carotenoids, and yellowness (b*) and flavonoids, Huang Tongchun (TF) was positively correlated to the contents of the relationship. (4) using illumnina/Solexa second generation high-throughput transcriptome sequencing technology of Calla Lily little black girl, romantic figure, fan pull the three varieties of transcriptome sequencing, received a total of 446340 high quality transcriptome short sequence, the average length of 785bp. notes in the Nr database with a total of 29816 Unigene related gene mapping, mapping the KEGG database to provide the reference directory, the 55 sequences may design color Calla flower color formation: combined with metabolism research on information and material metabolism, expounds Calla Lily anthocyanin metabolic pathway.

【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S682.264

【參考文獻】

相關(guān)期刊論文 前10條

1 鄒麗秋;王彩霞;匡雪君;李瀅;孫超;;黃酮類化合物合成途徑及合成生物學(xué)研究進展[J];中國中藥雜志;2016年22期

2 張振亞;裴翠明;馬進;;基于轉(zhuǎn)錄組和蛋白質(zhì)組關(guān)聯(lián)研究技術(shù)篩選紫花苜蓿耐鹽相關(guān)候選基因[J];植物生理學(xué)報;2016年03期

3 劉秀明;張宇;姚娜;崔喜燕;王立勇;董園園;王南;李曉薇;李海燕;李校X;;紅花(Carthamus tinctorius L.)不同開花時期的轉(zhuǎn)錄組測序及黃酮合成相關(guān)基因的驗證[J];中國細胞生物學(xué)學(xué)報;2015年09期

4 薛英利;趙慶師;黃由安;劉鐘;吳立勤;希從芳;李建賓;李紀元;;滇山茶的花色類型與細胞內(nèi)環(huán)境關(guān)系初探[J];云南農(nóng)業(yè)大學(xué)學(xué)報(自然科學(xué));2015年03期

5 戴思蘭;黃河;付建新;洪艷;;觀賞植物分子育種研究進展[J];植物學(xué)報;2013年06期

6 王小菁;楊玉萍;;我國觀賞花卉品質(zhì)形成的功能基因研究進展[J];植物學(xué)報;2013年05期

7 刁英;胡蓉;游永寧;鄭興飛;鄭興汶;謝克強;周明全;胡中立;;蓮屬植物葉片及花瓣的比較解剖學(xué)研究[J];湖北農(nóng)業(yè)科學(xué);2013年13期

8 謝燁;孫毅;黃繼榮;;擬南芥中花青素的修飾[J];植物生理學(xué)報;2013年02期

9 王卅;張e，

本文編號：1621395