發(fā)布時(shí)間：2018-03-05 03:40

  本文選題：玉米秸稈　切入點(diǎn)：預(yù)處理　出處：《沈陽農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：能源、資源、環(huán)境問題已成為制約社會(huì)經(jīng)濟(jì)可持續(xù)發(fā)展的主要障礙,開發(fā)新的可持續(xù)的綠色替代能源和資源已成為全球的緊迫任務(wù),利用木質(zhì)纖維素生產(chǎn)燃料乙醇,因其潔凈、環(huán)保和安全等優(yōu)點(diǎn),成為人們研究的熱點(diǎn)。利用木質(zhì)纖維素生產(chǎn)燃料乙醇,包括糖化過程和乙醇發(fā)酵過程,其中木質(zhì)纖維的預(yù)處理糖化過程是燃料乙醇生產(chǎn)過程中最重要的一環(huán)。本研究首先以玉米秸稈為供試材料,比較了四種不同預(yù)處理方法對玉米秸稈降解糖化過程的影響,這四種預(yù)處理方法分別為擠壓膨化與綠色木霉相結(jié)合預(yù)處理法、稀硫酸與綠色木霉相結(jié)合預(yù)處理法、雜色云芝/糙皮側(cè)耳與綠色木霉相結(jié)合預(yù)處理法及與單獨(dú)的綠色木霉處理法。通過玉米秸稈預(yù)處理試驗(yàn),以還原糖得率為主,確定了合適的玉米秸稈預(yù)處理方法。其次,研究了雜色云芝和糙皮側(cè)耳不同體積的混合配比對玉米秸稈降解效果的影響,并對雜色云芝/糙皮側(cè)耳最佳混配比時(shí)酶活隨時(shí)間的變化進(jìn)行了探討。最后,以酶解溫度、混合菌添加量、初始pH值和玉米秸稈粒徑為試驗(yàn)因素,考察了不同工藝條件下玉米秸稈降解過程中還原糖得率、纖維素降解率、半纖維素降解率和木質(zhì)素的降解率。得到的結(jié)論如下:(1)采用雜色云芝/糙皮側(cè)耳與綠色木霉相結(jié)合預(yù)處理法,在降解玉米秸稈過程中還原糖得率優(yōu)于擠壓膨化與綠色木霉相結(jié)合預(yù)處理法、稀硫酸與綠色木霉相結(jié)合預(yù)處理法及單獨(dú)的綠色木霉處理法。(2)雜色云芝/糙皮側(cè)耳按體積比1:1混合時(shí),在降解玉米秸稈過程中還原糖得率較其他混合比例稍高。(3)雜色云芝/糙皮側(cè)耳等體積比混合時(shí),漆酶(Lac)在培養(yǎng)10d時(shí)酶活最高,為2102U/L,過氧化物酶(Lip)在培養(yǎng)19d時(shí)最高,為215U/L。(4)通過正交旋轉(zhuǎn)試驗(yàn),明確了在溫度為28.9℃,pH值為5.3,混合菌添加量為31.9mL/L,秸稈粒徑為75目的條件下,玉米秸稈水解糖化時(shí)還原糖的得率最佳,還原糖得糖率為28.6%。通過本試驗(yàn),不但為平菇生產(chǎn)中剩余物的回收利用提供了有效途徑,而且也為玉米秸稈水解糖化生產(chǎn)燃料乙醇提供了 一定的參考。
[Abstract]:Energy, resources and the environment have become major obstacles to sustainable socio-economic development, and the development of new and sustainable green alternative sources of energy and resources has become an urgent global task, using lignocellulose to produce fuel ethanol because of its cleanliness, The advantages of environmental protection and safety have become the focus of research. Using lignocellulose to produce fuel ethanol, including saccharification process and ethanol fermentation process, The pretreatment and saccharification of wood fiber is the most important part in the process of fuel ethanol production. Firstly, the effects of four different pretreatment methods on the degradation and saccharification of corn straw were compared. The four pretreatment methods are extrusion expansion combined with green Trichoderma and dilute sulfuric acid combined with green Trichoderma. The pretreatment method was combined with Trichoderma viridis and Trichoderma viridis. Through the experiment of corn straw pretreatment, the suitable pretreatment method of corn stalk was determined by reducing sugar yield. In this paper, the effect of mixed ratio of different volume of hybrid lucidum and Pleurotus strigella on the degradation effect of corn straw was studied, and the change of enzyme activity with time in the optimum mixture ratio of the mixed ratio was discussed. Finally, the enzymatic hydrolysis temperature was used. The yield of reducing sugar and the degradation rate of cellulose in the process of corn straw degradation under different conditions were investigated by adding mixed bacteria, initial pH value and grain size of corn straw. The degradation rate of hemicellulose and lignin. The conclusion is as follows: (1) the pretreatment method of hybrid lucidum / Pleurotus strigella combined with Trichoderma viridis was used. The yield of reducing sugar in the degradation of corn straw was superior to the pretreatment method of extrusion and Trichoderma viride. The mixture of dilute sulfuric acid and Trichoderma viridis combined with the pretreatment method of Trichoderma viride and the treatment of Trichoderma viride alone. In the process of degradation of corn straw, the yield of reducing sugar was slightly higher than that of other mixtures. (3) when the volume ratio of mixed variegated lucidum / Pleurotus strigella was mixed, laccase Lacs had the highest enzyme activity at 10 days, which was 2102 U / L, and the peroxidase Lipase was the highest at 19 days. Under the conditions of temperature 28.9 鈩，

本文編號：1568616