本文關鍵詞：遼寧地區(qū)PRRS流行病學調查及其不同疫苗免疫對豬免疫效果影響的研究　出處：《沈陽農業(yè)大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 豬繁殖與呼吸綜合征病毒 血清學調查 病原學調查 細胞因子 血液細胞

【摘要】：本試驗旨在通過對遼寧地區(qū)豬繁殖與呼吸綜合征(PRRS)進行流行病學調查及對PRRS不同疫苗免疫對豬免疫效果的影響進行研究,為制定能夠有效防控PRRS的免疫程序提供科學依據。本次試驗應用ELISA檢測技術對2015年-2016年間遼寧地區(qū)10個市縣的已免疫與未免疫PRRS疫苗的豬血清樣品共1892份,進行PRRS血清流行病學調查。結果顯示:已免疫豬群PRRSV抗體陽性率為68.08%,不同生長時期中斷奶仔豬陽性率最高,不同季節(jié)中冬季陽性率最低;未免疫豬群PRRSV抗體陽性率為25.35%,不同生長時期中斷奶仔豬陽性率仍最高,不同季節(jié)中夏季陽性率最高。采集疑似PRRS病死豬的組織病料共1490份,進行PRRS的病原流行病學調查。結果顯示:2015年-2016年遼寧地區(qū)PRRS病原平均陽性率為45.91%,不同生長時期中以斷奶仔豬陽性率最高,其次是哺乳仔豬,不同季節(jié)中夏季陽性率最高。結果表明:PRRSV以仔豬最易感,氣溫低下是影響PRRSV免疫效果不佳的重要因素,同時氣溫高可以增加PRRSV的感染率。本試驗選取42頭未免健康斷奶仔豬,隨機分為4組(A組、B組、C組、D組)。A組對照,B組免疫弱毒苗,C組免疫滅活苗,D組同時免疫弱毒苗和滅活苗。分別于免疫前、免疫后第14 d、28 d、42 d、56 d、70 d前腔靜脈采血進行血常規(guī)分析,采用ELISA方法檢測特異性抗體,同時檢測淋巴細胞轉化率以及IFN-α IFN-γ、TNF-α等細胞因子水平。結果顯示:免疫后,D組白細胞數始終高于B組,并于免疫第42 d后極顯著高于其他3組(p0.01);免疫第28d后,D組淋巴細胞數極顯著高于B組(p0.01);各組紅細胞數、血紅蛋白濃度、中性粒細胞數及中值細胞數均呈現緩慢上升趨勢,且D組高于其他3組(p0.05);B組抗體水平顯著高于D組(p0.05)、極顯著高于A組(p0.01);在免疫后42 d時,D組淋巴細胞轉化率極顯著高于A組、B組、C組(p0.01),在免疫56 d后B組淋巴細胞轉化率顯著高于C、D兩組(p0.05);B組細胞因子水平于14 d時高于其他三組,加強免疫后細胞因子水平均有所下降,在70 d時升高。結果表明:同時免疫弱毒苗和滅活苗能夠更好的刺激豬血液細胞增殖,免疫藍耳病弱毒苗組免疫效果優(yōu)于免疫滅活苗組和兩種疫苗同時免疫組。
[Abstract]:The aim of this study was to investigate the epidemiology of porcine reproductive and respiratory syndrome (PRRSs) in Liaoning area and to study the effects of different vaccines of PRRS on the immune response of pigs. To provide a scientific basis for the formulation of an effective immunization program for the prevention and control of PRRS. This experiment applied ELISA detection technology to 10 cities and counties in Liaoning from 2015 to 2016 to be immunized and unimmunized. The serum samples of PRRS vaccine were 1 892. The results showed that the positive rate of PRRSV antibody in immunized pigs was 68.08, and the positive rate of weaning piglets was the highest in different growth stages. The positive rate in winter was the lowest in different seasons. The positive rate of PRRSV antibody in unimmunized pigs was 25.35. The positive rate of weaning piglets was still the highest in different growth stages. The positive rate in summer was the highest in different seasons. A total of 1490 tissue samples of suspected dead pigs with PRRS were collected. The results showed that the average positive rate of PRRS in Liaoning from 2015 to 2016 was 45.91%. The positive rate of weaning piglets was the highest in different growth stages, followed by suckling piglets, and the highest positive rate was in summer in different seasons. The results showed that weaning piglets were the most susceptible. Low temperature is an important factor that affects the immune effect of PRRSV, and high temperature can increase the infection rate of PRRSV. In this study, 42 healthy weaned piglets were randomly divided into 4 groups. Groups B, C, C, D, D, A, B, B, C, C, C, C, C, C, C, C, C, B, C, C, C, B, C, C, C, C, B, C, C, C, C, C, B, C, C, C, C, C, C, B, C, C, C, C, C, The blood samples were collected from the vena cava 70 days before 42 d to 56 d, and the specific antibodies were detected by ELISA method. The lymphocyte transformation rate and IFN- 偽 IFN- 緯 were also detected. The level of cytokines such as TNF- 偽 was significantly higher in group D than that in group B after immunization, and was significantly higher than that in group B (P 0.01) after 42 days of immunization. The number of lymphocytes in group D was significantly higher than that in group B after 28 days of immunization. The erythrocyte count, hemoglobin concentration, neutrophil number and median cell number of each group showed a slow upward trend, and group D was higher than other three groups (P 0.05). The level of antibody in group B was significantly higher than that in group D (p0.05), and was significantly higher than that in group A (p0.01). The lymphocyte transformation rate in D group was significantly higher than that in C group at 42 d after immunization, and the lymphocyte transformation rate in B group was significantly higher than that in C group on 56 d after immunization. Group D: p0.05; The levels of cytokines in group B were higher than those in the other three groups at day 14, and the levels of cytokines in group B decreased after immunization. The results showed that simultaneously immunized attenuated vaccine and inactivated vaccine could stimulate the proliferation of porcine blood cells better. The immune effect of the attenuated blue ear disease vaccine group was better than that of the inactivated vaccine group and the two vaccine groups.
【學位授予單位】：沈陽農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S858.28
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本文編號：1378255