本文關鍵詞：急性偏熱環(huán)境對肉雞行為、生理生化指標及avUCPmRNA表達影響　出處：《河北工程大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 急性偏熱環(huán)境 肉雞 行為 糖脂代謝 解偶聯(lián)蛋白

【摘要】：隨著室內溫濕調控技術的不斷改進,環(huán)境持續(xù)高溫對家禽應激影響相對減弱。但偏熱環(huán)境溫度驟然升高引起的急性應激現(xiàn)象比較常見,不利于家禽的健康生長。因此,急性偏熱環(huán)境對家禽生長發(fā)育的影響受到廣泛關注。本試驗研究急性偏熱環(huán)境對肉雞行為、生理和糖脂代謝、血液酸堿平衡及其胸肌和肝臟解偶聯(lián)蛋白的影響,旨在為肉雞提供熱舒適環(huán)境溫度及其調控研究奠定理論基礎和技術支持。本試驗設計環(huán)境溫度為23、26、29、32℃時,肉雞受急性應激30 min、2h后,研究其行為、生理和糖脂代謝、水鹽代謝及其胸肌和肝臟解偶聯(lián)蛋白指標變化;環(huán)境溫度為23℃,相對濕度為35%、60%、85%時,肉雞受急性應激2h,檢測上述同樣指標。主要試驗結果如下:1、不同環(huán)境溫度對肉雞行為、生理生化及解偶聯(lián)蛋白avUCP mRNA表達影響與20℃對照組相比,23℃急性偏熱處理,肉雞雞冠、耳垂、小腿、腳蹼溫度顯著升高(P0.05);伸展休息次數(shù)顯著性增多(P0.05),站立次數(shù)顯著性減少(P0.05);肉雞胸肌avUCP mRNA表達顯著性降低(P0.05)。26℃急性偏熱處理肉雞體核溫度和皮膚溫度、呼吸頻率均顯著性升高(P0.05),但TC無顯著性差異(P0.05);肉雞伸展休息次數(shù)顯著性增多(P0.05),坐著休息次數(shù)減少(P0.05);肉雞胸肌avUCP mRNA表達顯著性降低(P0.05)。29℃急性偏熱處理肉雞體核溫度和皮膚溫度、呼吸頻率均顯著性升高(P0.05),血清GLU、TC顯著性降低(P0.05);活動行為(采食、站立)次數(shù)顯著性減少(P0.05),飲水次數(shù)顯著性增多(P0.05);肉雞胸肌avUCP mRNA表達顯著性降低(P0.05),但肝臟表達升高(P0.05)。32℃急性熱處理肉雞體核溫度和皮膚溫度、呼吸頻率均顯著性升高(P0.05);血清TC含量顯著性降低(P0.05);坐著休息次數(shù)顯著性減少(P0.05);肉雞胸肌avUCP mRNA表達顯著性降低(P0.05),但肝臟表達升高(P0.05)。35℃急性熱處理肉雞皮溫、呼吸頻率均顯著性升高(P0.05);伸展休息次數(shù)顯著性增多(P0.05),坐著休息次數(shù)顯著性減少(P0.05)。這表明23℃時肉雞已開始通過行為調節(jié)來維持體內平衡,26℃時肉雞體核溫度升高,通過體核溫度升高來維持體內平衡,23℃時肉雞通過啟動化學調節(jié)來降低胸肌avUCP mRNA表達。2、同種溫度(23℃)不同濕度(35%、60%、85%)環(huán)境對肉雞行為、生理生化、糖脂代謝的影響急性偏熱23℃處理,與中濕(60%)相比,低濕(35%)處理肉雞體核、腳蹼溫度顯著性降低(P0.05),而小腿、耳垂溫度無顯著差異(P0.05);血清T_3、T_4、GLU顯著性降低(P0.05),TG、TC顯著性升高(P0.05),但CORT無顯著性差異(P0.05);呼吸頻率顯著性降低(P0.05);休息行為(坐著、伸展、俯伏)均無顯著性差異(P0.05)。高濕(85%)處理肉雞小腿、雞冠溫度顯著升高(P0.05);血漿T3顯著升高(P0.05),T4顯著降低(P0.05);血清GLU顯著升高(P0.05),血清TG、TC顯著升高(P0.05);呼吸頻率顯著升高(P0.05);坐著次數(shù)顯著降低(P0.05),伸展次數(shù)顯著增加(P0.05)。這表明23℃時,高濕(85%)環(huán)境顯著影響肉雞的休息行為、呼吸頻率、皮溫�？傊�,急性偏熱應激和不同濕度對AA肉雞行為、生理生化、分子指標均產生不同程度的影響。從生產性能和能量利用效率角度來看,23℃屬于偏熱溫度。
[Abstract]:With the continuous improvement of indoor temperature and humidity control technology, the impact of environmental continuous high temperature on poultry stress is relatively weakened. However, the acute stress caused by a sudden increase in the temperature of the heat environment is more common, which is not conducive to the healthy growth of poultry. Therefore, the effect of acute thermal environment on the growth and development of poultry is widely concerned. This experiment was conducted to study the effects of acute hot environment on behavior, physiology and glucose and lipid metabolism, blood acid-base balance and the uncoupling proteins in the chest muscle and liver of broilers, aiming to lay a theoretical foundation and technical support for the research of heating comfort environment temperature and its regulation in broilers. The experimental design of the environment temperature is 23, 26, 29, 32 degrees, 30 min, broiler acute stress by 2H, to study its behavior, physiology and metabolism of glucose and lipid, water and salt metabolism and uncoupling protein of breast muscle and liver index changes; the environment temperature is 23 DEG C, relative humidity of 35%, 60%, 85%, broiler under acute stress 2h, detection of the same index. The main results are as follows: 1, different environmental temperature on broiler behavior, physiology and biochemistry and uncoupling protein avUCP mRNA expression compared to the impact and 20 C 23 C control group, acute partial heat treatment, chicken comb, earlobe, leg, fin temperature increased significantly (P0.05); the rest was a significant increase in the number of extension (P0.05), the number of standing significant reduction (P0.05); the expression of avUCP was significantly lower in broiler breast muscle mRNA (P0.05). 26 degrees of acute partial heat treatment of broiler body core temperature and skin temperature and respiratory frequency were significantly increased (P0.05), but no significant difference in TC (P0.05); the rest was a significant increase in the number of broiler extension (P0.05), sit and rest; reduce the number (P0.05) significantly reduced the expression of avUCP mRNA P0.05 (chicken breast). 29 degrees of acute partial heat treatment of broiler body core temperature and skin temperature and respiratory frequency were significantly increased (P0.05), serum GLU, TC were significantly lower (P0.05); activities (feeding, standing) times were significantly decreased (P0.05), drinking times significantly increased (P0.05) was significantly reduced; chicken breast muscle avUCP mRNA (P0.05), but increased the expression of the liver (P0.05). 32 degrees of acute heat treatment of broiler body core temperature and skin temperature and respiratory rate were increased significantly (P0.05); the content of serum TC decreased significantly (P0.05); sit and rest times were significantly reduced (P0.05) significantly decreased the expression of chicken breast muscle; avUCP mRNA (P0.05), but increased the expression of the liver (P0.05). 35 degrees of acute heat treatment of broiler skin temperature and respiratory rate were increased significantly (P0.05); the rest was a significant increase in the number of extension (P0.05), sit and rest times were significantly reduced (P0.05). This indicates that when the temperature is 23, the broiler has begun to maintain homeostasis through behavioral adjustment. At 26? C, the body core temperature of broilers is increased, and the body balance is maintained through the increase of body core temperature. At 23? C, the expression of avUCP mRNA is reduced by starting chemical regulation in broilers. 2, the same temperature (23 DEG C) different humidity (35%, 60%, 85%) the environmental impact on broiler behavior, physiology and biochemistry, glucose and lipid metabolism in acute partial heat treatment and wet in 23, (60%) compared to low humidity (35%) broilers body nucleus, fins significantly lower temperature (P0.05), and the shank earlobe, temperature had no significant difference (P0.05); serum T_3, T_4 and GLU were significantly lower (P0.05), TG, TC increased significantly (P0.05), but no significant difference between CORT (P0.05); respiratory rate decreased significantly (P0.05); resting behavior (sit, stretch, and there were no) significant differences (P0.05). High humidity (85%) treatment of broiler leg, comb temperature increased significantly (P0.05); plasma T3 increased significantly (P0.05), T4 decreased significantly (P0.05); the serum GLU increased significantly (P0.05), serum TG, TC were significantly increased (P0.05); respiratory rate increased significantly (P0.05); the number was significantly reduced (sitting P0.05), stretching times increased significantly (P0.05). This shows that 23 degrees, high humidity environment (85%) significantly affect the resting behavior, respiratory rate, skin temperature of broilers. In conclusion, acute partial heat stress and different humidity have different effects on the behavior, physiology, biochemistry and molecular index of AA broilers. From the point of view of production performance and energy utilization efficiency, 23 C belongs to the temperature of partial heat.
【學位授予單位】：河北工程大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S831

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1 李香;急性偏熱環(huán)境對肉雞行為、生理生化指標及avUCPmRNA表達影響[D];河北工程大學;2017年

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本文編號：1345529