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【文章來源】：大連醫(yī)科大學遼寧省

【文章頁數(shù)】：113 頁

【學位級別】：博士

【文章目錄】：
Abstract
Abstract-Chinese
CHAPTER 1 Taurine Ameliorated Abnormalities and Nerve Dysfunction in Diabetic Rats
    1.Background
    2.Materials and Methods
        2.1. Ethics Statement
        2.2. Establishment of animal model
        2.3. Weight gain
        2.4. Feed Intake and Water Consumption
        2.5. Latency and Motor Conduction Velocity
        2.6. Blood Glucose
        2.7. Serum Insulin
        2.8. Insulin Resistance
        2.9. Biochemical Analysis of Blood Serum
        2.10. Electron Microscopy
    3.Results
        3.1. Taurine Improved Weight Gain in STZ Induced Diabetic Rats
        3.2. Taurine lowered blood glucose in STZ induced diabetic rats
        3.3. Taurine increased HDL-C and decreased LDL-C in STZ induced diabetic rats
        3.4. Taurine Decreased Triglycerides/Triacylglycerides in STZ-induced Diabetic rats
        3.5. Serum Electrophoresis
        3.6. Serum Insulin
        3.7. Insulin Resistance
        3.8. Taurine increased MCV and reduced latency
        3.9. Taurine improved morphology of spinal cord tissue and sciatic nerve tissue
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 2 Protective Effect of Taurine against Diabetes-induced Apoptosis in Spinal Cord of Rats
    1.Introduction
    2.Material and Methods
        2.1. Chemicals
        2.2. Ethics Statement
        2.3. Establishment of Animal Model
        2.4. Detection of Apoptosis
        2.5. Assay for Cytochrome c (cyt-c)
        2.6. Determination of Cyt-c Protein Expression
        2.7. Western blotting
        2.8. Determination of Caspase-3 activity
        2.9. Statistical analysis
    3.Results
        3.1. Taurine Prevented Cell Death in Diabetic Rats
        3.2. Taurine Activated Akt and Phosphorylated Bad
        3.3. Bax and Bcl-2 Expression
        3.4. Taurine Reduced the Release of Cytochrome c
        3.5. Taurine Reduced Activation of Caspase-3
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 3 Effect of Taurine on mi RNA profile of Diabetic Rats
    1.Introduction
    2.Materials and Methods
        2.1. Statement of Ethics
        2.2. Animal Model
        2.3. RNA Extraction and Sequencing
        2.4. Searching for mi RNAs Significant Profiles
        2.5. Predicating the Functions of mi RNA Targets
    3.Results
        3.1. Construction of Small RNA Libraries by Sequencing
        3.2. Determination of mi RNAs Expression in Diabetic rats with or without TaurineTreatment
        3.3. Category Analysis of Specific mi RNAs in Taurine Treated Diabetic Rats
        3.4. Annotating the Function of Specific mi RNAs and Targets in Taurine Treated DiabeticRats
    4.Discussion
    5.Conclusion
    6.References
CHAPTER 4 General Background and review of literature
    1.Diabetes
    2.Taurine
        2.1. Effect of Taurine on Glucagon Activity
        2.2. Taurine Modifies Glucose Level in Diabetics
        2.3. Taurine Improves Hyperglycemia
        2.4. Taurine Improves Insulin Resistance and Secretion
    3.Diabetes Induced Complications
        3.1. Taurine Protects Brain Damage in Diabetes
            3.1.1. Taurine Protects Diabetic Neuropathy
            3.1.2. Taurine Protects Diabetic Retinopathy
        3.2. Diabetes Damages Excretory System
            3.2.1. Taurine Protects Diabetic Nephropathy
        3.3. Diabetes Damages Digestive System Organs
            3.3.1. Taurine Protects Diabetic Liver Damage
        3.4. Diabetes Damages Circulatory System
            3.4.1. Taurine Protects Diabetic Vascular Problems
            3.4.2. Taurine Protects Diabetic Heart Problems
    4.Taurine Protects Against Oxidative Stress
    5.Oxidative Stress, Taurine Mechanism
        5.1. Advanced Glycation Endproducts
        5.2. PI3Kinase/AKT pathway
        5.3. Mitochondrial Apoptosis Pathway
    6.References
ABBREVIATIONS
ACKNOWLEDGEMENT
List of Papers


【參考文獻】：
期刊論文
[1]Taurine protects against retinal and optic nerve damage induced by endothelin-1 in rats via antioxidant effects[J]. Natasha Najwa Nor Arfuzir,Renu Agarwal,Igor Iezhitsa,Puneet Agarwal,Sabrilhakim Sidek,Nafeeza Mohd Ismail.  Neural Regeneration Research. 2018(11)



本文編號：3550625