近年來(lái),納米醫(yī)學(xué)及其相關(guān)研究正在飛速發(fā)展。全球基于納米醫(yī)學(xué)的產(chǎn)業(yè)規(guī)模亦快速擴(kuò)張,目前已形成估值1600億美元的龐大市場(chǎng)。納米技術(shù)在納米醫(yī)學(xué)中有廣泛的應(yīng)用,包括藥物輸送,納米造影劑,納米生物材料,納米藥物和醫(yī)學(xué)納米機(jī)器人等。功能性納米材料的特性對(duì)納米醫(yī)學(xué)應(yīng)用的影響是納米醫(yī)學(xué)研究的重要方向。前人研究表明,納米材料的尺度、形狀和表面功能化等理化特性均不同程度影響細(xì)胞對(duì)其的攝取效率。硅納米材料由于自身良好的生物相容性,優(yōu)異的半導(dǎo)體性能和深厚的產(chǎn)業(yè)基礎(chǔ)在藥物遞送,納米傳感器,納米機(jī)器人等納米醫(yī)學(xué)研發(fā)方向上極具應(yīng)用前景。其中硅納米棒作為一種高縱橫比的一維材料,被細(xì)胞攝取的機(jī)制尚未明晰。本研究通過(guò)新型刻蝕技術(shù)方案制作直徑和長(zhǎng)度可控的硅納米棒,并對(duì)其表面涂覆腫瘤細(xì)胞膜蛋白功能化;同時(shí),結(jié)合成像流式、熒光共聚焦顯微鏡和電鏡等技術(shù)系統(tǒng)定量分析硅納米棒細(xì)胞攝取效率。本研究集中探討了表面功能化對(duì)硅納米棒細(xì)胞攝取的影響。我們通過(guò)多種不同方式證明細(xì)胞膜蛋白提取物確實(shí)涂覆于硅納米棒表面。透射電鏡直接觀測(cè)到經(jīng)涂覆處理的硅納米棒上出現(xiàn)19.09 ± 0.47 nm的薄層。蛋白銀染檢測(cè)涂覆硅納米棒與膜蛋白提取物有一致的條... 

【文章來(lái)源】：南京大學(xué)江蘇省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：117 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
Abstract
Abbreviations
Chapter I Literature Reviews
    1.1 Nanomedicine and Nano-Bio interface
    1.2 Physicochemical properties of nanoparticles
        1.2.1 Size effect on nano-bio interface
        1.2.2 Shape effect on nano-bio interface
        1.2.3 Surface modification to improve cellular internalization
    1.3 Mechanisms of Cellular Internalization
        1.3.1 Phagocytosis
        1.3.2 Clathrin-mediated endocytosis
        1.3.3 Caveolae-mediated endocytosis
        1.3.4 Clathrin- and caveolae-independent endocytosis
    1.4 Interactions at nano-bio interface
        1.4.1 the Solid-Liquid microenvironment in the context of in vitro cell culture
    1.5 Silicon nanorod
        1.5.1 Fabrication techniques of Silicon nanorod
        1.5.2 Recent progress in SiNR-cell interface research
    1.6 Thesis statement & Justification
Chapter Ⅱ Systemic analysis the cell internalization efficiency of cell membrane proteinscoated SiNR
    2.1 Introduction
    2.2 Materials and Methods
    2.3 Results
        2.3.1 Fabrication of Size-Controllable Silicon Nanorod
        2.3.2 Surface modification of SiNR with Membrane Protein
        2.3.3 Membrane Proteins indeed coated on the surface of SiNR
        2.3.4 SiNR Internalization Efficiency Is also Determined by Target Cells
    2.4 Discussion
Chapter Ⅲ Exploring cell intrinsic factors affected on cell membrane proteins coated siliconnanorod
    3.1 Introduction
    3.2 Materials and Methods
    3.3 Results
        3.3.1 Cell Membrane Proteomics Analysis and Gene Ontology on Cancer CellInternalization
        3.3.2 ATXN2 Is a Negative Regulator of Nanorod Internalization
    3.4 Discussion
Reference
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