本文選題：病毒性心肌炎 + CD45~+CD34~+VLA-4~+細(xì)胞��； 參考：《廣西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討在急性病毒性心肌炎小鼠發(fā)病過程中,是否存在骨髓來源CD45~+CD34~+VLA-4~+細(xì)胞動員及其意義。方法:1、應(yīng)用流式細(xì)胞術(shù)檢測CD45~+CD34~+VLA-4~+細(xì)胞在心肌、外周血和骨髓中的變化;2、免疫組化法檢測心肌組織中定植因子VCAM-1的表達(dá)情況;3、實時熒光定量PCR法檢測心肌組織中VCAM-1與炎癥因子IL-1β、TNF-αmRNA的相對表達(dá)量;4、Western-blotting法檢測定VCAM-1與IL-1β、TNF-α蛋白的相對表達(dá)量?結(jié)果:1、急性病毒性心肌炎小鼠心肌中CD45~+CD34~+VLA-4~+細(xì)胞的表達(dá)與對照組比較,第3天升高,第7天達(dá)到高峰,之后開始下降,第14天和第28天仍高于對照組(P0.05);外周血中及骨髓中CD45~+CD34~+VLA-4~+細(xì)胞的表達(dá)與對照組比較,第3天降低,但之后開始升高,第7天達(dá)到高峰,隨后下降,第14天和第28天仍高于對照組(P0.05)。2、免疫組化示急性病毒性心肌炎小鼠心肌炎癥細(xì)胞的周圍VCAM-1的相對表達(dá)量明顯增加,且伴多量的新生毛細(xì)血管生成。VCAM-1與心肌組織中的CD45~+CD34~+VLA-4~+細(xì)胞呈正相關(guān)關(guān)系。3、實時熒光定量PCR及Western-blotting示急性病毒性心肌炎小鼠心肌中VCAM-1及TNF-α、IL-1β的表達(dá)量明顯高于對照組(P0.05)。VCAM-1與IL-1β、TNF-α的mRNA表達(dá)量呈正相關(guān)關(guān)系,VCAM-1的蛋白表達(dá)量與心肌組織中的CD45~+CD34~+VLA-4~+細(xì)胞呈正相關(guān)關(guān)系。4、動員到心肌組織中的CD45~+CD34~+VLA-4~+細(xì)胞與急性病毒性心肌炎心肌中的炎癥因子及病理積分不成線性關(guān)系。結(jié)論:在病毒性心肌炎小鼠中,VCAM-1促進(jìn)CD45~+CD34~+VLA-4~+細(xì)胞動員到心肌中,可能參與急性病毒性心肌炎的發(fā)病過程及心肌組織的再生與修復(fù)。
[Abstract]:Objective: To investigate whether there is mobilization and significance of bone marrow derived CD45~+CD34~+VLA-4~+ cells in the pathogenesis of acute viral myocarditis. Methods: 1, the changes of CD45~+CD34~+VLA-4~+ cells in the myocardium, peripheral blood and bone marrow were detected by flow cytometry; and 2, the expression of colonization factor VCAM-1 in myocardial tissue was detected by immunohistochemistry. 3, the relative expression of VCAM-1 and inflammatory factors IL-1 beta, TNF- alpha mRNA in myocardium was detected by real time fluorescence quantitative PCR. 4, Western-blotting method was used to detect the relative expression of VCAM-1 and IL-1 beta and TNF- alpha protein? Results: 1, the expression of CD45~+CD34~+VLA-4~+ cells in the myocardium of acute viral myocarditis was compared with the control group, third days up. High, seventh days reached the peak, then began to decline, fourteenth days and twenty-eighth days is still higher than the control group (P0.05), the expression of CD45~+CD34~+VLA-4~+ cells in the peripheral blood and bone marrow compared with the control group, third days lower, but then began to rise, the seventh day to reach the peak, then decreased, fourteenth days and twenty-eighth days is still higher than the control group (P0.05).2, immunohistochemistry showed urgent. The relative expression of VCAM-1 in the myocarditis and myoinflammatory cells of the mice with sexual viral myocarditis increased significantly, and the number of new capillary generated.VCAM-1 was positively related to the CD45~+CD34~+VLA-4~+ cells in the myocardium,.3, real-time fluorescent quantitative PCR and Western-blotting showed VCAM-1 and TNF- in the myocardium of acute viral myocarditis in mice The expression of alpha, IL-1 beta was significantly higher than that of the control group (P0.05).VCAM-1 and IL-1 beta, and the mRNA expression of TNF- a was positively correlated. The expression of VCAM-1 protein was positively correlated with CD45~+CD34~+VLA-4~+ cells in myocardial tissue. It was mobilized to the CD45~+CD34~+VLA-4~ + cells in the myocardium and the cause of inflammation in the myocardium of acute viral myocarditis. Conclusion: in the mice with viral myocarditis, VCAM-1 promotes the mobilization of CD45~+CD34~+VLA-4~+ cells into the myocardium, and may be involved in the pathogenesis of acute viral myocarditis and the regeneration and repair of myocardial tissue.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R542.21

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本文編號：2116788