本文選題：野丁香屬 + 薄皮木��； 參考：《鄭州大學(xué)》2017年碩士論文

【摘要】：背景:野丁香屬(Leptodermis Wall.)是茜草科(Rubiaceae)下的一個(gè)屬,約40種,分布于喜馬拉雅區(qū)至日本。根據(jù)《中國(guó)植物志》記載,在我國(guó)有35種,9變種,1變型,南北均有分布,但主產(chǎn)地為華北部、西南部部分省份。其中薄皮木(Leptodermis oblonga Bge.)是茜草科野丁香屬的一種落葉灌木,產(chǎn)于我國(guó)華北、西南地區(qū),常生長(zhǎng)于山坡、路邊等向陽(yáng)處,也見(jiàn)于灌木叢中。據(jù)研究記載,薄皮木因花型似丁香花,又叫野丁香,因株型矮小,夏秋開(kāi)花,在草坪、園林等作為觀賞性植物種植,具有觀賞價(jià)值。薄皮木的干枯、鮮嫩枝葉常被作為中低等飼用植物被牛羊等畜牧采食。因其植物化學(xué)成分含有較高粗脂肪含量,為牲蓄提供大量能量和營(yíng)養(yǎng),所以其具有飼用價(jià)值。目前,薄皮木的研究主要集中在種子萌發(fā)特性、葉表皮形態(tài)特征、快速繁殖技術(shù)與組織培養(yǎng)等內(nèi)容,對(duì)該植物化學(xué)成分與藥理作用的研究報(bào)道很少。鑒于以上發(fā)現(xiàn),前期課題組對(duì)其地上部分的正丁醇部位和乙酸乙酯部位的化學(xué)成分做了初步研究。研究發(fā)現(xiàn),正丁醇部位分離出6個(gè)化合物,乙酸乙酯部位分離出11個(gè)化合物,分別是降倍半萜苷類(lèi)、三萜類(lèi)、黃酮類(lèi)、甾體類(lèi)、木脂素類(lèi)和其他類(lèi);為完善薄皮木的研究,我們主要對(duì)薄皮木乙酸乙酯部位的化學(xué)成分及藥理活性進(jìn)行了進(jìn)一步的研究。目的意義:本文對(duì)薄皮木乙酸乙酯部位的化學(xué)成分進(jìn)行研究,以期明確該植物中的化學(xué)成分,分離、鑒定單體化合物,對(duì)結(jié)構(gòu)新穎可能存在藥理活性的化合物進(jìn)行活性研究,為后期薄皮木植物資源開(kāi)發(fā)應(yīng)用提供理論依據(jù)。方法:本文采用正相硅膠柱、反相ODS柱、反相MCI柱和Sephadex LH-20凝膠柱等柱填料進(jìn)行化學(xué)成分分離純化,并應(yīng)用理化常數(shù)測(cè)定和現(xiàn)代波譜技術(shù)對(duì)所分離得到的化合物進(jìn)行結(jié)構(gòu)鑒定;利用NO一步法試劑盒對(duì)薄皮木不同極性大小的粗提物及分離得到的單體化合物進(jìn)行抗炎活性研究。結(jié)論:(1)從薄皮木乙酸乙酯部位中分離鑒定出了15個(gè)化合物,13個(gè)化合物為首次從薄皮木中分離鑒定出來(lái),其中黃酮類(lèi)5個(gè),三萜類(lèi)6個(gè),甾體類(lèi)2個(gè),其他類(lèi)2個(gè)。分別是3,4',5,7-tetrahydroxy flavone(1)、3-oxo-oleanolic acid(2)、kaempferol-3-O-β-D-glucopyranoside(3)、isorhamnetin-3-O-β-Dglucopyranoside(4)、3,5,7-trihydroxy-4'-methoxy flavone(5)、betulinic acid(6)、quercetin(7)、1-octacosanol(8)、β-daucosterol(9)、ursolic aldehyde(10)、trilinolein(11)、β-sitosterol(12)、friedelane-2α,3β-diols(13)、3β-acetoxy-urs-11-en-28,13β-olide(14)、3β-hydroxyurs-12-ene(15)。(2)薄皮木總浸膏及不同極性部位浸膏對(duì)LPS刺激的RAW264.7小鼠巨噬細(xì)胞有不同程度的抑制作用,結(jié)果顯示,相比于模型組,Et OAC Fr.和Bu OH Fr.作用的NO釋放量有所減少(P0.05),其余不同組分作用的NO釋放量隨濃度的增加而減少,但抑制作用較弱,無(wú)顯著性差異(P0.05);相比不同部位抑制率發(fā)現(xiàn),抑制作用較強(qiáng)的為乙酸乙酯部位和正丁醇部位,濃度為200μg/m L時(shí),抑制率分別為56.06%、57.03%,因此,乙酸乙酯部位和正丁醇部位可能含有具有抗炎作用的活性物質(zhì)。除了化合物1-octacosanol(8)和trilinolein(11)之外,其余的單體化合物對(duì)LPS誘導(dǎo)的RAW264.7細(xì)胞釋放的NO均有一定的抑制作用,但抑制作用較弱,與模型組對(duì)比,kaempferol-3-O-β-D-glucopyranoside(3),3,5,7-trihydroxy-4'-methoxyflavone(5),betulinic acid(6),β-daucosterol(9),ursolic aldehyde(10),β-sitosterol(12),3β-hydroxyurs-12-ene(15)在濃度為25μg/m L時(shí),有顯著性差異(P0.05),其余化合物無(wú)顯著性差異(P0.05),且具有劑量依賴(lài)性。
[Abstract]:Background: Leptodermis Wall. (Leptodermis Wall.) is a genus of alizardae (Rubiaceae), about 40 species, distributed in the Himalaya region to Japan. According to the records of Chinese flora, there are 35 species, 9 varieties, 1 variants, and the north and south of China, but the main origin is the North China and the southwest part of the province. And Bopimu (Leptodermis oblonga Bge.) is alizari. A deciduous shrub of the genus lilaca of the genus lilaca, native to North China and southwest of China, often growing on hillsides, along the side of the side of the road, and also in the shrubs. According to research, the thin pellicus is like lilacs and is called No Choka. The plant type is small and flowering in summer and autumn. It is planted in lawn and gardens as ornamental plants, and has ornamental value. Thin skin wood. The fresh shoot leaves are often used as medium and low feed plants to be fed by cattle and sheep. Because the chemical composition of the plant contains high crude fat content, it provides a large amount of energy and nutrition for the animals, so it has a feeding value. At present, the research of thin skin is mainly concentrated on the seed germination characteristics, the leaf surface morphology, rapid propagation technology and the rapid propagation technology. There are few reports on the chemical composition and pharmacological action of the plant. In view of the above discovery, preliminary research group made a preliminary study on the chemical composition of n-butanol part and ethyl acetate part of the upper part of the plant. It was found that 6 compounds were separated from n-butanol and 11 compounds were separated from ethyl acetate. Compounds are terpenoid glycosides, three terpenoids, flavonoids, flavonoids, steroids, lignans and other classes. In order to improve the research of thin skinned wood, we mainly study the chemical composition and pharmacological activity of the ethyl acetate part of thin skin wood. The purpose is to study the chemical composition of the ethyl acetate part of the thin skin wood in this paper. The chemical components in the plant are identified, separated and identified, and the activity of the compounds with novel structure may exist in the study. The theoretical basis for the development and application of late thin skin wood plant resources is provided. Methods: This article uses the column of the normal silica gel, the reverse phase ODS column, the reverse phase MCI column and the Sephadex LH-20 gel column. The chemical constituents were isolated and purified, and the compounds were identified by physical and chemical constants and modern spectroscopy. The NO one step kit was used to study the anti-inflammatory activity of the crude extracts of different polar sizes and the isolated monomers. (1) separation from the ethyl acetate part of pellicle. 15 compounds were identified and 13 compounds were isolated and identified for the first time from thin skin wood, including 5 flavonoids, 6 three terpenoids, 2 steroids, 2 other classes, 3,4', 5,7-tetrahydroxy flavone (1), 3-Oxo-oleanolic acid (2), kaempferol-3-O- beta -D-glucopyranoside (3), isorhamnetin-3-O- beta -Dglucopyranoside (4), 3,5,7-tr. Ihydroxy-4'-methoxy flavone (5), betulinic acid (6), quercetin (7), 1-Octacosanol (8), beta -daucosterol (9), ursolic aldehyde (10), trilinolein (11), beta -sitosterol (12), friedelane-2 alpha, 3 beta, 13, 3 beta (14), 3 beta (15)). PS stimulated RAW264.7 mouse macrophages had varying degrees of inhibition. The results showed that the amount of NO release of Et OAC Fr. and Bu OH Fr. decreased (P0.05), while the NO release of the other components decreased with the increase of concentration, but the inhibitory production was weak and no significant difference (P0.05) was compared with the different parts. The rate of inhibition was found to be stronger in ethyl acetate and n-butanol. When the concentration was 200 g/m L, the inhibition rate was 56.06%, 57.03%. Therefore, ethyl acetate and n-butanol may contain anti-inflammatory substances. Besides compounds 1-Octacosanol (8) and trilinolein (11), the rest of the monomers The inhibition effect on NO released by LPS induced RAW264.7 cells was certain, but the inhibitory effect was weak. Compared with the model group, kaempferol-3-O- beta -D-glucopyranoside (3), 3,5,7-trihydroxy-4'-methoxyflavone (5), betulinic acid (6), beta -daucosterol (9), ursolic aldehyde (10), beta -sitosterol (12), 3 beta (15) were in the concentration of 2. There was a significant difference (P0.05) between 5 g/m L and the other compounds (P0.05), and it was dose-dependent.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R284.1

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相關(guān)碩士學(xué)位論文 前2條

1 王文錦;薄皮木乙酸乙酯部位化學(xué)成分及抗炎活性的研究[D];鄭州大學(xué);2017年

2 王穎慧;薄皮木的化學(xué)成分研究[D];鄭州大學(xué);2014年

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本文編號(hào)：2029263