發(fā)布時間：2018-06-12 17:37

  本文選題：支氣管哮喘 + ADAM33基因��； 參考：《新疆醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討烏魯木齊地區(qū)支氣管哮喘兒童ADAM33基因V4位點多態(tài)性、維生素D(VitD)水平及其相關(guān)性研究,期望從基因水平為指導喘患兒的防治提供理論依據(jù)。方法:選取同期5-15歲于烏魯木齊地區(qū)生活的哮喘患兒154人(其中漢族111,維族43),同時設(shè)立對照組健康非哮喘兒童120人(漢族64,維族56),首先采用PCR聚合酶鏈反應對ADAM 33基因V4位點進行單核苷酸多態(tài)性(SNP)分型,并用ELISA法檢測VitD水平,進一步根據(jù)民族進行組內(nèi)分層,進行V4位點SNP、VitD分析并進行相關(guān)性分析。結(jié)果:(1)V4位點3種基因型頻率分布在病例組和對照組間差異有統(tǒng)計學意義(x2=6.806,P=0.033),在不同民族間差異無統(tǒng)計學意義(實驗組:x2=0.714,P=0.699;對照組:x2=5.898,P=0.052),考慮ADAM 33基因V4位點與烏魯木齊地區(qū)維吾爾族、漢族兒童哮喘發(fā)病均不相關(guān)。(2)VitD水平檢測病例組與對照組數(shù)值有差異,差異有統(tǒng)計學意義(t=2.341,P=0.020)。(3)ADAM 33基因V4位點與VitD水平相關(guān)性分析。結(jié)論:(1)ADAM33基因V4位點與烏魯木齊地區(qū)支氣管哮喘兒童發(fā)病有相關(guān)性,但無民族間差異。V4位點突變后的G基因可能是支氣管哮喘發(fā)病的危險因素。(2)VitD水平與支氣管哮喘兒童發(fā)病有關(guān)。(3)ADAM 33基因V4位點不影響VitD水平。
[Abstract]:Objective: to investigate the V4 polymorphism of ADAM33 gene and vitamin D Vitamin D level in children with bronchial asthma in Urumqi. Methods: 154 asthmatic children aged 5 to 15 years living in Urumqi were selected (111 Han, 43 Uygur) and 120 healthy non-asthmatic children (Han 64, Uygur 56) in the same period. PCR polymerase chain reaction (PCR) was used to detect Adam. Single nucleotide polymorphisms (SNPs) were performed at V4 locus of 33 gene. The level of Vit D was detected by Elisa, and further stratified according to ethnic group, the V4 SNPV VitD was analyzed and the correlation analysis was carried out. Results the frequencies of three genotypes at the V4 locus were significantly different between the case group and the control group, but there was no significant difference among the different nationalities (experimental group: x20.714, P0. 699; control group: x2 + 5. 898P0. 052), considering the V4 locus of ADAM33 gene and Uygur nationality in Urumqi region. The level of VitD in asthmatic children of Han nationality was not related to the incidence of asthma. The V4 locus of ADAM33 gene and the level of VitD were significantly different between the case group and the control group, and the difference was statistically significant between the V4 locus of ADAM33 gene and the level of VitD. Conclusion the V4 locus of ADAM33 gene is associated with the incidence of bronchial asthma in Urumqi. However, the G gene after mutation of V4 locus in different nationalities may be the risk factor of asthma. The V4 locus of ADAM33 gene has no effect on the level of VitD in children with bronchial asthma.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R725.6

【參考文獻】

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本文編號：2010521