本文選題：血管性癡呆　切入點：線粒體　出處：《吉林大學》2017年碩士論文

【摘要】：目的:探討人參皂苷Re對慢性缺血性血管癡呆(VD)大鼠海馬神經元缺血損傷和線粒體功能障礙的保護作用,為開發(fā)安全有效的防治VD藥物提供實驗依據。方法:應用分次離斷結扎雙側頸總動脈法建立VD大鼠模型;利用Morris水迷宮和HE染色判定模型構建效果;采用試劑盒提取各組線粒體及蛋白質,并通過電鏡技術、Western-blot實驗和H_2O_2試劑盒比較各組差異。利用真空泵和CO_2孵箱建立缺氧復氧模型,采用LDH釋放量檢測、DNA瓊脂糖電泳及細胞形態(tài)觀察驗證模型建立效果。進一步通過檢測各組LDH釋放量、DNA斷裂程度及細胞狀態(tài),并通過提取線粒體和蛋白質和應用Western Blot和H_2O_2試劑盒研究人參皂苷Re的作用。結果:分次離斷結扎大鼠雙側頸總動脈后,大鼠認知能力下降,逃避潛伏期明顯延長。細胞形態(tài)結構顯示,大鼠海馬神經元排列紊亂,細胞周圍呈空泡化,失去正常結構。COX IV、PDH-A1和H_2O_2的表達結果顯示,不同濃度的人參皂苷Re對大鼠VD線粒體的COX IV和PDH-A1的表達均有明顯促進作用,而對H_2O_2釋放量有一定抑制作用,表明人參皂苷Re可修復海馬神經元的線粒體損傷。大鼠海馬神經元經缺氧復氧后,其模型組LDH釋放量增高、DNA斷裂加劇、神經元突起退化及胞體固縮;而人參皂苷Re可有效抑制LDH釋放、DNA斷裂及突觸退化,促進對線粒體COX IV和PDH-A1的表達,抑制H_2O_2的釋放。結論:人參皂苷Re可對腦缺血所造成的海馬神經元線粒體損傷起保護作用。人參皂苷Re可通過改善海馬神經元的線粒體損傷,改善大鼠的認知能力。
[Abstract]:Objective: to investigate the protective effect of ginsenoside re on hippocampal neuronal ischemia injury and mitochondrial dysfunction in rats with chronic ischemic vascular dementia (VD), and to provide experimental evidence for the development of safe and effective drugs for the prevention and treatment of VD.Methods: VD rat model was established by severing and ligating bilateral common carotid artery, Morris water maze and HE staining were used to determine the effect of model construction, mitochondria and protein were extracted from each group by kit.Western blot and H_2O_2 kit were used to compare the differences.The model of anoxia and reoxygenation was established by vacuum pump and CO_2 incubator. The effect of the model was verified by using LDH release quantity to detect agarose electrophoresis and observe cell morphology.The effect of ginsenoside re on the activity of ginsenoside re was studied by detecting the amount of LDH released in each group and the cell state, and by extracting mitochondria and proteins, and using Western Blot and H_2O_2 kit to study the effect of ginsenoside re.Results: the cognitive ability of bilateral common carotid artery was decreased and the escape latency was prolonged.The morphological structure of the cells showed that the hippocampal neurons in the rats were disordered and vacuolated around the cells, and the expression of PDH-A1 and H_2O_2 were found to be lost in the normal structure.Different concentrations of ginsenoside re could obviously promote the expression of COX IV and PDH-A1 in VD mitochondria, but inhibit the release of H_2O_2, suggesting that ginsenoside re could repair the mitochondrial damage of hippocampal neurons.After hypoxia and reoxygenation, the release of LDH in the model group increased, the neurite degeneration and cell body pyknosis were aggravated, while ginsenoside re could effectively inhibit the LDH release and synaptic degeneration.Promote the expression of mitochondrial COX IV and PDH-A1 and inhibit the release of H_2O_2.Conclusion: ginsenoside re can protect hippocampal neuron mitochondria from cerebral ischemia.Ginsenoside re can improve the cognitive ability of rats by improving mitochondria damage of hippocampal neurons.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R285.5

【參考文獻】

相關期刊論文 前4條

1 陳美華;吳月鵬;張顏波;金毅;;人參皂苷Rg2對腦缺血再灌注大鼠神經保護作用的研究[J];中風與神經疾病雜志;2014年12期

2 張振宇;侯祥紅;孟姍姍;李丹;張向楠;李彬;王基野;柯濤;張文斌;駱文靜;;丙酮酸對細胞寒冷應激損傷的保護作用研究[J];現(xiàn)代生物醫(yī)學進展;2013年20期

3 王洪財;蔣玉萌;趙雪;王寧;高迪;關銘;張驚宇;楊子超;;人參皂苷Rb1對Aβ淀粉樣蛋白誘導大鼠海馬神經元損傷的保護作用[J];吉林大學學報(醫(yī)學版);2012年03期

4 ;Hippocampal mitochondrial cytochrome C oxidase activity and gene expression in a rat model of chronic cerebral ischemia[J];Neural Regeneration Research;2011年32期

，

本文編號：1719765