本文選題：乳腺癌　切入點：腸道菌群　出處：《新疆醫(yī)科大學》2017年碩士論文

【摘要】：目的:1)采用IlluminaMiseq高通量測序技術,分別對維吾爾族和漢族乳腺癌患者與其對應的健康組婦女糞便樣品進行腸道菌群的結構分析,研究維吾爾族和漢族乳腺癌患者各自腸道菌群結構及其多樣性特征。探索不同民族腸道菌群與乳腺癌可能存在的相關性。初步構建不同民族乳腺癌腸道菌群構成情況,嘗試篩選出與疾病顯著相關的菌屬。2)采用同種分析方法對漢族乳腺癌婦女和維吾爾族乳腺癌婦女腸道菌群進行差異性分析,初步探索兩民族乳腺癌婦女腸道菌群在多樣性及結構上的異同。方法:通過Illumina Miseq平臺分別對漢族和維吾爾族乳腺癌婦女和其對應的健康婦女的16SrDNA V6-V8區(qū)進行高通量測序,測序結果進行OTU聚類分析、多樣性及結構分析得出兩個民族患者腸道菌群結構、豐度及多樣性分布。結果:1)比較OTUs維吾爾族乳腺癌組(n=10)和健康對照組(n=10)的平均OTUs數(shù)目無顯著差異(P0.05);漢族乳腺癌組(n = 25)的平均OTUs數(shù)目明顯低于健康對照組(n=15)(P0.05)。利用稀釋曲線、Chao1 指數(shù)、Shannon 指數(shù)和 Rank abundance 曲線等進行四組樣本的復雜度分析,測序量足以覆蓋腸道內(nèi)各細菌種屬,各組樣品豐富度和均勻度較好。2)在屬水平上,維吾爾族乳腺癌婦女與健康組存在顯著性差異的菌屬是擬桿菌屬、毛螺菌科和桿菌屬(P0.05);漢族乳腺癌婦女與健康組存在顯著性差異的菌屬是SubdoligranuLum菌屬、Alistipes菌屬、Blautia菌屬、糞球菌屬_3等在內(nèi)的13個菌屬(P0.05);維吾爾族乳腺癌組與漢族乳腺癌組中存在顯著性差異的菌屬是普氏菌屬_9、擬桿菌屬、Succinivibrio 菌屬、SubdoligranuLum、Parabacteroides、Ruminococcus_2 等在內(nèi)的19類菌屬(P0.05);維吾爾族正常組與漢族正常組間存在顯著性差異的菌屬是擬桿菌屬、Megamonas菌屬、巨球型菌屬、雙歧桿菌屬等在內(nèi)的7個菌屬(P0.05)。3)維吾爾族乳腺癌婦女組與健康婦女組、漢族乳腺癌婦女組與健康婦女組、漢族乳腺癌婦女組與維吾爾族乳腺癌婦女組、漢族健康婦女組與維吾爾族健康婦女組腸道菌群在各分類水平上均存在結構上的差異,且每一個組在每一個水平上均有其優(yōu)勢物種。結論:1)兩民族乳腺癌患者與健康對照婦女腸道菌群之間存在著結構上的顯著變化,提示腸道菌群結構上的這種變化和乳腺癌的疾病狀態(tài)可能有某些關聯(lián)。2)漢族乳腺癌婦女與維吾爾族乳腺癌婦女腸道菌群在結構組成上存在著差異,這種差異可能是遺傳因素和環(huán)境因素共同作用的結果。3)健康漢族與健康組吾爾族婦女腸道菌群在結構比例上也存在著顯著的差異,這種結果可能與基因型及飲食結構的不同有所關聯(lián)。
[Abstract]:Objective: to analyze the intestinal flora of the fecal samples of women with breast cancer in Uygur and Han nationality and their corresponding healthy women by using IlluminaMiseq high-throughput sequencing technique.To study the structure and diversity of intestinal flora in Uygur and Han patients with breast cancer.To explore the possible correlation between intestinal microflora and breast cancer in different nationalities.The intestinal flora of breast cancer of Han nationality and Uygur nationality was analyzed by using the homologous analysis method, and the intestinal microflora of breast cancer of different nationalities was preliminarily constructed, and the bacteria related to the disease were screened out. 2) the difference of intestinal microflora between Han and Uygur breast cancer women was analyzed by the same method.To explore the diversity and structure of intestinal flora of breast cancer women.Methods: the 16SrDNA V6-V8 region of Han and Uygur breast cancer women and their corresponding healthy women were analyzed by high throughput sequencing using Illumina Miseq platform. The results were analyzed by OTU cluster analysis.The distribution of intestinal flora, abundance and diversity in patients with two nationalities were obtained by the analysis of diversity and structure.Results there was no significant difference in the average number of OTUs between the OTUs Uygur breast cancer group (n = 10) and the healthy control group (P 0.05), but the average OTUs number in the Han nationality breast cancer group (n = 25) was significantly lower than that in the healthy control group (P 0.05).The complexity analysis of four groups of samples was carried out by using the dilution curve Chao1 index Shannon index and Rank abundance curve. The sequencing amount was enough to cover the species and genera of bacteria in the intestinal tract, and the sample richness and evenness of each group was better than that of the other groups at the generic level.There were significant differences between the Uygur breast cancer women and the healthy group in the genera of Bacteroides, Helicobacteriaceae and Bacteriaceae (P0.05A), while the genera of SubdoligranuLum, Alistipes, Blautia, which were significantly different between the women of Han nationality and the healthy group, were found in this study.There are 13 strains of Staphylococcus spp. P0.05A, the genus of P0.05in Uygur breast cancer group and Han nationality breast cancer group, the genus P0.05in the normal Uygur group, and the subdoligranacteroides Parabacteroides Ruminococcus2 in the subdoligranacteroides Ruminococcus sp. 2 in the normal group of Uygur nationality, and the genus 9 in the genus Precinivibrio and the other 19 species in the genus Subdoligrania granuloca, Parabacteroides, Ruminococcus2, etc., respectively.The genus Megamonas, which has significant difference between the Han nationality and the normal group, is the genus Megamonas.The seven bacteria of the genus Bifidobacterium and megagonal type belong to P0.05. 3) Women with breast cancer of Uygur nationality and healthy women, women with breast cancer of Han nationality and healthy women, women with breast cancer of Han nationality and women with breast cancer of Uygur nationality.There were structural differences in intestinal flora between healthy women of Han nationality and Uygur nationality healthy women groups, and each group had its dominant species at each level.Conclusion (1) there were significant structural changes in intestinal flora between breast cancer patients and healthy women.It is suggested that there may be some correlation between the changes of intestinal flora structure and the disease status of breast cancer. 2) there are differences in the composition of intestinal microflora between women with breast cancer of Han nationality and women with breast cancer of Uygur nationality.This difference may be the result of the combination of genetic factors and environmental factors. 3) there are significant differences in the proportion of intestinal flora between healthy Han and healthy women of Wuer nationality.The results may be associated with different genotypes and dietary patterns.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.9

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