本文關(guān)鍵詞： 隆起糜爛性胃炎 胃癌 Runx3 P21　出處：《福建中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的與意義:通過檢測(cè)Runx3、P21蛋白在不同分型隆起糜爛性胃炎及胃癌組織中的表達(dá)情況,分析Runx3蛋白與P21蛋白在不同胃黏膜組織中表達(dá)的相關(guān)性,探討不同分型隆起糜爛性胃炎與胃癌的關(guān)系及可能存在的癌變分子機(jī)制,為今后隆起糜爛性胃炎的臨床干預(yù)提供更多的理論依據(jù)。研究方法:收集2016年2月至2016年12月于福建中醫(yī)藥大學(xué)附屬人民醫(yī)院消化內(nèi)鏡科行電子胃鏡檢查的符合研究要求的病例,分為慢性淺表性胃炎(chronic superficial gastritis,CSG)、隆起糜爛性胃炎(raised erosive gastritis,REG)未成熟型、REG 成熟型及胃癌(gastric cancer,GC)四組,每組各30例,通過免疫組織化學(xué)染色SP法檢測(cè)Runx3、P21蛋白在四組患者胃黏膜組織中的表達(dá)情況。結(jié)果:1.REG未成熟型組及REG成熟型組的萎縮發(fā)生率分別為30.0%(9/30)、43.3%(13/30),腸上皮化生(簡(jiǎn)稱腸化)發(fā)生率分別為13.3%(4/30)、36.7%(11/30),上皮內(nèi)瘤變發(fā)生率分別為3.3%(1/30)、13.3%(4/30),REG成熟型組腸化發(fā)生率顯著高于REG未成熟型組(P0.05),而兩組胃黏膜的萎縮及上皮內(nèi)瘤變發(fā)生率均無顯著差異(P0.05)。2.CSG組、REG未成熟型組、REG成熟型組及GC組的Runx3蛋白表達(dá)陽性率逐漸下降,分別為:80.0%(24/30)、73.3%(22/30)、43.3%(13/30)、36.7%(11/30)。REG成熟型組Runx3蛋白表達(dá)的陽性率顯著低于CSG組及REG未成熟型組(P0.05),但與GC組相比,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。CSG組Runx3蛋白表達(dá)的陽性率顯著高于REG成熟型組及GC組(P0.05),但與REG未成熟型組相比,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3.CSG組、REG未成熟型組、REG成熟型組及GC組的P21蛋白表達(dá)陽性率分別為:70.0%(21/30)、60.0%(18/30)、36.7%(11/30)、33.3%(10/30),呈逐漸下降趨勢(shì)。REG成熟型組P21蛋白表達(dá)的陽性率顯著低于CSG組(P0.05),但與GC組及REG未成熟型組表達(dá)相比,差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。CSG組P21蛋白表達(dá)的陽性率顯著高于REG成熟型組及GC組(P0.05),而與REG未成熟型相比,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。4.REG成熟型組Runx3蛋白表達(dá)與腸上皮化生有相關(guān)性,且呈負(fù)相關(guān)(r=-0.386,P0.05);REG未成熟型組Runx3蛋白表達(dá)與腸上皮化生無相關(guān)性(r=-0.207,P0.05)。5.Runx3與P21蛋白在CSG組及REG未成熟型組的表達(dá)均無相關(guān)性(r= 0.218,P0.05;r= 0.277,P0.05);Runx3與P21蛋白在REG成熟型組的表達(dá)有相關(guān)性,且呈正相關(guān)(r= 0.591,P0.05);二者在GC組的表達(dá)亦有相關(guān)性,且呈正相關(guān)(r= 0.636,P0.05)。結(jié)論:Runx3和P21蛋白在胃癌組織中表達(dá)明顯下降或缺失,提示Runx3和P21蛋白可能共同參與了胃黏膜癌變的過程;Runx3和P21蛋白在成熟型隆起糜爛性胃炎組織中表達(dá)亦明顯下降或缺失,提示成熟型的隆起糜爛性胃炎已經(jīng)具有癌變的潛能,需要臨床早期干預(yù)。
[Abstract]:Objective and significance: by detecting the expression of Runx3p21 protein in erosive gastritis and gastric carcinoma, the relationship between Runx3 protein and P21 protein expression in different gastric mucosa was analyzed. To explore the relationship between erosive gastritis and gastric cancer and the possible molecular mechanism of carcinogenesis. To provide more theoretical basis for the clinical intervention of erosive gastritis in the future. Methods: to collect the coincidence of electronic gastroscopy in the Department of Digestive Endoscopy from February 2016 to December 2016 in the people's Hospital affiliated to Fujian University of traditional Chinese Medicine. The cases required by the study, The patients were divided into four groups: chronic superficial gastritis, raised erosive gastritis, immature type and gastric cancer with 30 cases in each group. Immunohistochemistry SP method was used to detect the expression of Runx3P21 protein in gastric mucosa of four groups of patients. Results 1. The incidence of atrophy in immature group and mature group of REG was 30.010 / 30 and 13.3 / 30 respectively, intestinal metaplasia occurred in intestinal metaplasia (abbreviated as intestinal metaplasia). The incidence of intraepithelial neoplasia was 3.3 / 30, respectively, and the incidence of intestinal metaplasia in the mature type group was significantly higher than that in the immature REG group, while there was no significant difference between the two groups in the incidence of gastric mucosal atrophy and intraepithelial neoplasia. 2. There was no significant difference in the incidence of gastric mucosal atrophy and intraepithelial neoplasia between the two groups. The positive rate of Runx3 protein expression in mature group and GC group decreased gradually. The positive rate of Runx3 protein expression in 13 / 30 / 30 and 36.7% mature type group was significantly lower than that in CSG group and REG immature type group (P 0.05), but compared with GC group, the positive rate of Runx3 protein expression was significantly lower than that in CSG group and REG immature type group. The positive rate of Runx3 protein expression in Runx3 group was significantly higher than that in REG mature type group and GC group, but it was significantly higher than that in REG immature type group. The positive rate of P21 protein expression in the mature type group and GC group were 70.021 / 30 and 60.018 / 30 respectively. The positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression was significantly lower in the mature type group than in the CSG group, and the positive rate of P21 protein expression was significantly lower than that in the control group and the GC group. Compared with the expression of REG immature group, The positive rate of P21 protein expression in P0.05G group was significantly higher than that in REG mature type group and GC group, but there was no significant difference compared with REG immature type. 4. There was no correlation between the expression of P21 protein and intestinal metaplasia in mature type group. There was no correlation between the expression of Runx3 protein and intestinal metaplasia in CSG group and REG immature type group. There was no correlation between the expression of P21 protein and the expression of P21 protein in REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group. There was also a positive correlation between the expression of the two proteins in GC group, and a positive correlation between them. Conclusion the expression of the protein of: Runx3 and P21 is significantly decreased or absent in gastric carcinoma. These results suggest that Runx3 and P21 proteins may be involved in the process of gastric mucosal carcinogenesis. The expression of Runx3 and P21 proteins in mature protuberance erosive gastritis is also decreased or absent, suggesting that mature protuberance erosive gastritis has the potential of carcinogenesis. Early clinical intervention is needed.
【學(xué)位授予單位】：福建中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.2;R573.3

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